What is the target of this antibody?

This antibody targets VEGFR-1 (Vascular Endothelial Growth Factor Receptor 1), also known as FLT1. It binds to the receptor which plays a key role in vascular development and regulation of permeability.

Is this antibody suitable for immunoprecipitation?

Yes, this recombinant monoclonal antibody is suitable for Immunoprecipitation (IP). Additionally, it has been validated for use in Immunofluorescence (IF), Neutralization (Neut), Functional Studies (FuncS), ELISA, Flow Cytometry (FC), and Immunocytochemistry (ICC), offering broad versatility for your research needs.

Anti-Human VEGFR-1 Recombinant Antibody (TAB-177)

CAT#: TAB-177

Recombinant monoclonal antibody to VEGFR-1. It is a human monoclonal antibody designed for the treatment of solid tumors.

Tested Data Gene Expression

ELISA

Figure 1 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in ELISA

ELISA analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag). Then blocked with BSA and incubated with TAB-177. A HRP-conjugated goat anti-human IgG was used as the secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in WB

Western blot analysis of TAB-177 was performed by loading human VEGFR-1 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-177 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in DB

Dot Blot analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag).
TAB-177 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/90_G3_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/1368_A5_2_blue_green.jpg

Normal Tissue Figure 3 Testis

Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/142518_A_6_6.jpg

RNA Expression Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1

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Specifications

Immunogen
Recombinant human soluble extracellular Flt-1 Ig-like loop 1 to 5 (sFlt-1(D5)).

Host Species
Human

Derivation
Human

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, ICC and most other immunological methods.

MW
146.8 kDa

Related Disease
Colon cancers

Product Property

Purity
>95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.

Target

Alternative Names
FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; vascular endothelial growth factor receptor 1; VEGFR1; FLT-1; VEGFR-1; fms-like tyrosine kinase 1; tyrosin

Gene ID
2321

UniProt ID
P17948

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Breast cancer biomarkers at key points during disease progression

Key Tool For Angiogenesis Research

This human monoclonal antibody against VEGFR-1 (FLT1) is a key tool in our angiogenesis research. We used it for Immunofluorescence and Neutralization assays. It binds the receptor specifically and helps us study the VEGF signaling pathways involved in vascular development and permeability.

Q&As

What is the target of this antibody?

A: This antibody targets VEGFR-1 (Vascular Endothelial Growth Factor Receptor 1), also known as FLT1. It binds to the receptor which plays a key role in vascular development and regulation of permeability.
Is this antibody suitable for immunoprecipitation?

A: Yes, this recombinant monoclonal antibody is suitable for Immunoprecipitation (IP). Additionally, it has been validated for use in Immunofluorescence (IF), Neutralization (Neut), Functional Studies (FuncS), ELISA, Flow Cytometry (FC), and Immunocytochemistry (ICC), offering broad versatility for your research needs.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-177, RRID: AB_3111850)

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Biosimilar Overview

Please refer to Icrucumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Icrucumab.

Related Diseases

Rheumatoid Arthritis

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-177.pdf

MSDS

MSDS - TAB-177.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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