Anti-Human VEGFR-1 Recombinant Antibody (Icrucumab) (CAT#: TAB-177)

Figure 1 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in ELISA

ELISA analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag). Then blocked with BSA and incubated with TAB-177. A HRP-conjugated goat anti-human IgG was used as the secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in WB

Western blot analysis of TAB-177 was performed by loading human VEGFR-1 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-177 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in DB

Dot Blot analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag).
TAB-177 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control