Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16) (CAT#: PABL-677)
Recombinant Mouse Antibody (mAb J16) is capable of binding to PCSK9. This antibody potently inhibits PCSK9 binding to the LDLR extracellular domain and PCSK9-mediated down-regulation of LDLR in vitro.
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Figure 1 Human serum titrated with anti-PCSK9 mAb J16.
Raw data trace of fluorescence (in Volts) as a function of time recorded by the KinExA instrument for a typical experiment.
Bee, C., Abdiche, Y. N., Pons, J., & Rajpal, A. (2013). Determining the binding affinity of therapeutic monoclonal antibodies towards their native unpurified antigens in human serum. PLoS One, 8(11), e80501.
Figure 2 PCSK9 follows both direct and indirect internalization routes.
Internalization of J16 bound PCSK9 in liver in negative or APLP2 siRNA treated mice. Scale bars, 10 μM.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 3 ApoB/LDL effects on PCSK9 internalization and function.
Representative western blot showing APLP2, LDLR, ApoB, Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 4 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.
Western blot showing APLP2, PCSK9, or Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 5 ELISA showing LDLR-ECD coated plates.
Shown as average of triplicate samples with SD. J16 effect shown as dotted line.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Specifications
- Immunogen
- Human proprotein convertase subtilisinkexin type 9
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PCSK9
- Species Reactivity
- Human
- Clone
- mAb J16
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- PCSK9; proprotein convertase subtilisinkexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisinkexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisinkexin type 9 preproprotein
- Gene ID
- 255738
- UniProt ID
- Q8NBP7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H31 | Human Anti-PCSK9 Recombinant Antibody (TAB-H31) | FC, IP, ELISA, Neut, FuncS, IF, IHC | Human IgG2, λ |
| TAB-0953CL | Human Anti-PCSK9 Recombinant Antibody (TAB-0953CL) | ELISA | Human IgG1 |
| TAB-0953CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0953CL-F(E)) | ELISA | Human Fab |
| TAB-0954CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0954CL-F(E)) | WB | Human Fab |
| TAB-0955CL-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0955CL-F(E)) | WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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