Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16) (CAT#: PABL-677)
Recombinant Mouse Antibody (mAb J16) is capable of binding to PCSK9. This antibody potently inhibits PCSK9 binding to the LDLR extracellular domain and PCSK9-mediated down-regulation of LDLR in vitro.
Specific Inquiry
Figure 1 Human serum titrated with anti-PCSK9 mAb J16.
Raw data trace of fluorescence (in Volts) as a function of time recorded by the KinExA instrument for a typical experiment.
Bee, C., Abdiche, Y. N., Pons, J., & Rajpal, A. (2013). Determining the binding affinity of therapeutic monoclonal antibodies towards their native unpurified antigens in human serum. PLoS One, 8(11), e80501.
Figure 2 PCSK9 follows both direct and indirect internalization routes.
Internalization of J16 bound PCSK9 in liver in negative or APLP2 siRNA treated mice. Scale bars, 10 μM.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 3 ApoB/LDL effects on PCSK9 internalization and function.
Representative western blot showing APLP2, LDLR, ApoB, Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 4 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.
Western blot showing APLP2, PCSK9, or Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 5 ELISA showing LDLR-ECD coated plates.
Shown as average of triplicate samples with SD. J16 effect shown as dotted line.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Specifications
- Immunogen
- Human proprotein convertase subtilisinkexin type 9
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PCSK9
- Species Reactivity
- Human
- Clone
- mAb J16
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- PCSK9; proprotein convertase subtilisinkexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisinkexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisinkexin type 9 preproprotein
- Gene ID
- 255738
- UniProt ID
- Q8NBP7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H31 | Human Anti-PCSK9 Recombinant Antibody (TAB-H31) | FC, IP, ELISA, Neut, FuncS, IF, IHC | Human IgG2, λ |
| TAB-0959CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0959CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0953CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0953CL-F(E)) | ELISA | Human Fab |
| TAB-0954CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0954CL-F(E)) | WB | Human Fab |
| TAB-0959CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0959CL-F(E)) | ELISA, FC | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-758 | Anti-PCSK9 Recombinant Antibody (Lodelcizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-764CL | Human Anti-PCSK9 Recombinant Antibody (TAB-764CL) | ELISA | Human IgG |
| TAB-0020CL | Anti-Human PCSK9 Recombinant Antibody (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
| TAB-0957CL | Anti-Human PCSK9 Recombinant Antibody (h7F8) | ELISA | Humanized antibody |
| TAB-0958CL | Anti-Human PCSK9 Recombinant Antibody (h7F8ml2) | ELISA | Humanized antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-294 | Human Anti-PCSK9 Recombinant Antibody (clone mAb1) | WB, Block, FuncS | Human IgG |
| HPAB-0234-YC | Mouse Anti-PCSK9 Recombinant Antibody (HPAB-0234-YC) | ELISA, FuncS | Mouse IgG |
| HPAB-0240-WJ | Mouse Anti-PCSK9 Recombinant Antibody (HPAB-0240-WJ) | ELISA | Mouse IgG |
| HPAB-0241-WJ | Mouse Anti-PCSK9 Recombinant Antibody (HPAB-0241-WJ) | ELISA | Mouse IgG |
| HPAB-0242-WJ | Mouse Anti-PCSK9 Recombinant Antibody (HPAB-0242-WJ) | ELISA | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-670 | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (PFBL-670) | WB, FuncS | Human Fab |
| PFBL-671 | Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| PFBL-672 | Human Anti-PCSK9 Recombinant Antibody (clone CBL36Q); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-0810-CN-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-0810-CN-F(E)) | ELISA | Human Fab |
| HPAB-667-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-667-FY-F(E)) | Inhib | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0956CL | Anti-Human PCSK9 Recombinant Antibody (m7F8) | ELISA | |
| TAB-0960CL | Anti-Human PCSK9 Recombinant Antibody (Ab1) | ELISA | Rabbit mAb |
| TAB-0956CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (m7F8) | ELISA | |
| TAB-0960CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (Ab1) | ELISA | Rabbit mAb |
| TAB-0956CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (m7F8) | ELISA |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0080CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKE2) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0081CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKF8) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0082CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKG1) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0083CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (P1.70) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0084CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKE1) | FuncS, Inhib, FC, WB | Single domain antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1826CQ | Recombinant Human Anti-PCSK9 Antibody, Unconjugated | Neut | IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2618 | Hi-Affi™ Recombinant Rabbit Anti-PCSK9 Monoclonal Antibody (DS2618AB) | WB | IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-758 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Lodelcizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-H10 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Bococizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
| AFC-TAB-H59 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Ralpancizumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-H31 | Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | Human IgG1, λ |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1383-FY-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1383-FY-F(E)) | Block, Inhib | Mouse Fab |
| HPAB-1384-FY-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1384-FY-F(E)) | Block, Inhib | Mouse Fab |
| HPAB-1385-FY-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1385-FY-F(E)) | Block, Inhib | Mouse Fab |
| HPAB-1386-FY-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1386-FY-F(E)) | Block, Inhib | Mouse Fab |
| HPAB-1387-FY-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1387-FY-F(E)) | Block, Inhib | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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