Rabbit Anti-ARNT Recombinant Antibody (VS3-CJ838) (CAT#: VS3-CJ838)

Name: Rabbit Anti-ARNT Recombinant Antibody (VS3-CJ838)
SKU: VS3-CJ838
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes human ARNT.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/30_F11_2_selected.jpg

Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/ihc_selected.jpg

Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/29_F11_1_red_green.jpg

Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/31_F11_1_red_green.jpg

Normal Tissue

Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_B_8_5.jpg

Figure 6 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_3.jpg

Figure 7 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_5.jpg

Figure 8 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_4_6.jpg

Figure 9 Placenta
(Decidual cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_2_7.jpg

Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_8.jpg

RNA Expression

Figure 11 RNA cell line category: Cell line enriched (U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143437-ARNT

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human ARNT

Species Reactivity
Human

Applications
WB, IHC

Conjugate
Unconjugated

Product Property

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
40% Glycerol, 1% BSA, TBS, pH7.4.

Preservative
0.05% Sodium Azide

Storage
Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Western Blot, Immunohistochemistry.

Target

Alternative Names
HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta

Gene ID
405

UniProt ID
P27540

Cellular Localization
Nucleus

Protein Refseq
NP_001184254.1; NP_001272964.1; NP_001272965.1

Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
DrMAB-214	Human Anti-ARNT Recombinant Antibody (clone U39)	IP, IF	Human IgG1
MOB-0917CT	Mouse Anti-ARNT Recombinant Antibody (clone EML171)	IF, IHC-P, WB	Mouse IgG2a
MRO-0735-CN	Rabbit Anti-ARNT Recombinant Antibody (clone CBACN-269)	WB, IHC	Rabbit IgG
ZG-0102C	Mouse Anti-ARNT Recombinant Antibody (clone 4C5)	WB, IHC-P, IF	Mouse IgG
VS3-QX65	Mouse Anti-ARNT Recombinant Antibody (clone 7E7-6F6-1G2)	WB, IHC	Mouse IgG1

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