Rabbit Anti-ARNT Recombinant Antibody (VS3-CJ838)
CAT#: VS3-CJ838
This product is a rabbit antibody that recognizes human ARNT.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/30_F11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/29_F11_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1759/31_F11_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_4_6.jpg
Normal Tissue
Figure 9 Placenta
Decidual cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_2_7.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4318/11331_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143437-ARNT
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ARNT
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry.
Target
- Alternative Names
- HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
- Gene ID
- 405
- UniProt ID
- P27540
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001184254.1; NP_001272964.1; NP_001272965.1
- Function
- Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ARNT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-214 | Human Anti-ARNT Recombinant Antibody (clone U39) | IP, IF | Human IgG1 |
| MOB-0917CT | Mouse Anti-ARNT Recombinant Antibody (clone EML171) | IF, IHC-P, WB | Mouse IgG2a |
| MRO-0735-CN | Rabbit Anti-ARNT Recombinant Antibody (clone CBACN-269) | WB, IHC | Rabbit IgG |
| ZG-0550J | Rabbit Anti-ARNT Recombinant Antibody (clone 2F11) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-QX65 | Mouse Anti-ARNT Recombinant Antibody (clone 7E7-6F6-1G2) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY173 | Anti-ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY530 | Anti-Mouse ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY529 | Anti-Human ARNT Immunohistochemistry Kit | IHC | |
| VS-0525-XY531 | Anti-Rat ARNT Immunohistochemistry Kit | IHC |
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