Human Anti-B. anthracis PA Recombinant Antibody (W1) (CAT#: HPAB-0747-YJ)
This product is a recombinant Chimeric (chimpanzee/human) antibody that recognizes Bacillus anthracis PA. It may serve as PA entry inhibitors for use in the emergency prophylaxis against and treatment of anthrax.
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ELISA
Figure 1 ELISA titration of anti–protective antigen (PA)
Recombinant PA or LF or unrelated proteins, bovine serum albumin (BSA), thyroglobulin, lysozyme, and phosphorylase b were used to coat the wells of an ELISA plate. Wells then were incubated with various dilutions of scFvs. Bound scFv was detected by the addition of peroxidase-conjugated anti-His antibody, followed by tetramethylbenzidine substrate. The anti-PA and antiLF scFvs did not bind to the unrelated proteins; only BSA is shown as an example. OD, optical density measured at 450 nm.
Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.
Neut
Figure 2 In vitro neutralization assay.
Anti–protective antigen (PA) IgG was mixed with anthrax toxin and was incubated for 1 h at 37°C. The mixture was added to RAW264.7 cells in a 96-well plate and was incubated for 4 h at 37°C. After washing, the cells were stained with MTT dye, and then lysis was performed in a solution containing 0.5% SDS in 90% isopropanol and 0.05 mol/L HCl. The plate was read at an optical density measured at 570 nm, and the cell survival was calculated relative to untreated cells. Results were plotted and analyzed using Prism software (version 4; Graphpad Software). EC50, effective concentration for 50% neutralization. ■, W1; ▼, W2; □, 14B7.
Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.
Specifications
- Immunogen
- Recombinant PA
- Host Species
- Human
- Derivation
- Chimeric (chimpanzee/human)
- Type
- Chimeric (chimpanzee/human) IgG1, κ
- Specificity
- B. anthracis
- Species Reactivity
- B. anthracis
- Clone
- W1
- Applications
- ELSIA, Neut
- Related Disease
- Anthrax
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in ELSIA, Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Protective antigen; Bacillus anthracis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single Domain Antibody Products
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-194-VHH | Recombinant Anti-B. anthracis PA VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
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