Human Anti-B. anthracis PA Recombinant Antibody (W1) (CAT#: HPAB-0747-YJ)

Figure 1 ELISA titration of anti–protective antigen (PA)

Recombinant PA or LF or unrelated proteins, bovine serum albumin (BSA), thyroglobulin, lysozyme, and phosphorylase b were used to coat the wells of an ELISA plate. Wells then were incubated with various dilutions of scFvs. Bound scFv was detected by the addition of peroxidase-conjugated anti-His antibody, followed by tetramethylbenzidine substrate. The anti-PA and antiLF scFvs did not bind to the unrelated proteins; only BSA is shown as an example. OD, optical density measured at 450 nm.

Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.

Figure 2 In vitro neutralization assay.

Anti–protective antigen (PA) IgG was mixed with anthrax toxin and was incubated for 1 h at 37°C. The mixture was added to RAW264.7 cells in a 96-well plate and was incubated for 4 h at 37°C. After washing, the cells were stained with MTT dye, and then lysis was performed in a solution containing 0.5% SDS in 90% isopropanol and 0.05 mol/L HCl. The plate was read at an optical density measured at 570 nm, and the cell survival was calculated relative to untreated cells. Results were plotted and analyzed using Prism software (version 4; Graphpad Software). EC50, effective concentration for 50% neutralization. ■, W1; ▼, W2; □, 14B7.

Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.