This product is a recombinant Chimeric (chimpanzee/human) antibody that recognizes Bacillus anthracis PA. It may serve as PA entry inhibitors for use in the emergency prophylaxis against and treatment of anthrax.
Figure 1 ELISA titration of anti–protective antigen (PA)
Recombinant PA or LF or unrelated proteins, bovine serum albumin (BSA), thyroglobulin, lysozyme, and phosphorylase b were used to coat the wells of an ELISA plate. Wells then were incubated with various dilutions of scFvs. Bound scFv was detected by the addition of peroxidase-conjugated anti-His antibody, followed by tetramethylbenzidine substrate. The anti-PA and antiLF scFvs did not bind to the unrelated proteins; only BSA is shown as an example. OD, optical density measured at 450 nm.
Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.
Figure 2 In vitro neutralization assay.
Anti–protective antigen (PA) IgG was mixed with anthrax toxin and was incubated for 1 h at 37°C. The mixture was added to RAW264.7 cells in a 96-well plate and was incubated for 4 h at 37°C. After washing, the cells were stained with MTT dye, and then lysis was performed in a solution containing 0.5% SDS in 90% isopropanol and 0.05 mol/L HCl. The plate was read at an optical density measured at 570 nm, and the cell survival was calculated relative to untreated cells. Results were plotted and analyzed using Prism software (version 4; Graphpad Software). EC50, effective concentration for 50% neutralization. ■, W1; ▼, W2; □, 14B7.
Chen, Z., Moayeri, M., Zhou, Y. H., Leppla, S., Emerson, S., Sebrell, A., ... & Purcell, R. (2006). Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen. The Journal of infectious diseases, 193(5), 625-633.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-194-VHH | Recombinant Anti-B. anthracis PA VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-224 | Anti-B. Anthracis Anthrax Protective Antigen (Raxibacumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - lambda |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-97 | Recombinant Human Anti-B. anthracis PA Antibody | Neut, IF, FuncS | IgG |
MRO-513-MZ | Mouse Anti-B. anthracis PA Recombinant Antibody (clone 7825) | ELISA | Mouse IgG |
HPAB-0780-CN | Mouse Anti-B. anthracis PA Recombinant Antibody (HPAB-0780-CN) | ELISA, Neut | Mouse IgG1, κ |
HPAB-0781-CN | Mouse Anti-B. anthracis PA Recombinant Antibody (HPAB-0781-CN) | ELISA, Neut | Mouse IgG1, κ |
VS3-QX94 | Mouse Anti-B. anthracis PA Recombinant Antibody (clone 5C1-C8-H8) | ELISA | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-97-S(P) | Recombinant Human Anti-B. anthracis PA Antibody scFv Fragment | Neut, WB, FuncS | scFv |
HPAB-0780-CN-S(P) | Mouse Anti-B. anthracis PA Recombinant Antibody; scFv Fragment (HPAB-0780-CN-S(P)) | ELISA, Neut | Mouse scFv |
HPAB-1826-FY-F(E) | Human Anti-B. anthracis PA Recombinant Antibody (clone 4H7); scFv Fragment | ELISA | Human scFv |
FAMAB-0048JF-S(P) | Human Anti-B. anthracis PA Recombinant Antibody (clone 83K7C); scFv Fragment | WB, ELISA, FuncS | Human scFv |
FAMAB-0223JF-S(P) | Monkey Anti-B. anthracis PA Recombinant Antibody (clone 35PA83); scFv Fragment | ELISA, FuncS | Monkey scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0387LC | Recombinant Mouse Anti-B. anthracis PA Antibody (F20G75) | BL, ELISA | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0780-CN-F(E) | Mouse Anti-B. anthracis PA Recombinant Antibody; Fab Fragment (HPAB-0780-CN-F(E)) | ELISA, Neut | Mouse Fab |
HPAB-0781-CN-F(E) | Mouse Anti-B. anthracis PA Recombinant Antibody; Fab Fragment (HPAB-0781-CN-F(E)) | ELISA, Neut | Mouse Fab |
HPAB-1646-FY-S(P) | Human Anti-B. anthracis PA Recombinant Antibody; scFv Fragment (HPAB-1646-FY-S(P)) | ELISA | Human scFv |
HPAB-1647-FY-S(P) | Human Anti-B. anthracis PA Recombinant Antibody; scFv Fragment (HPAB-1647-FY-S(P)) | ELISA | Human scFv |
HPAB-1648-FY-S(P) | Human Anti-B. anthracis PA Recombinant Antibody; scFv Fragment (HPAB-1648-FY-S(P)) | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-224 | Afuco™ Anti-B. anthracis PA ADCC Recombinant Antibody (Raxibacumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-440CQ | Afuco™ Anti-PA ADCC Recombinant Antibody (Obiltoxaximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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