Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10)
CAT#: FN-032CQ
This product is a Mouse antibody that recognizes CD28. The antibody can be used for immunoassay techniques such as: ELISA, FC, FuncS.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Immunogen
- Recombinant Human CD28 and HPB-MLT human tumor cells
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- ANC28.1/5D10
- Applications
- ELISA, FC, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Flow Cytometry Compatible
The ANC28.1/5D10 antibody provided clear and specific results in flow cytometry for analyzing CD28 expression and T-cell activation markers, significantly improving our data quality.
Consistent Performance
The consistent performance of ANC28.1/5D10 across various experiments has made it a staple in our laboratory. Its high specificity and reproducibility are essential for our immunological research.
Valuable Autoimmune Research Tool
ANC28.1/5D10 has been essential in our autoimmune disease research. It's helped explore CD28 signaling's role in autoimmune T-cell activity, providing valuable insights into disease mechanisms.
Q&As
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In lab trials, how can the specificity of the ANC28.1/5D10 antibody be confirmed?
A: Specificity of ANC28.1/5D10 can be validated using flow cytometry, western blotting, or ELISA with appropriate positive control cells expressing CD28 and negative controls lacking CD28 expression.
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In what ways does the ANC28.1/5D10 clone support research on TCR signaling pathways?
A: The ANC28.1/5D10 antibody provides the necessary co-stimulatory signal via CD28 engagement, enabling detailed exploration of how co-stimulation influences TCR signaling and downstream pathways.
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What extra safety measures need to be followed while working with the ANC28.1/5D10 antibody in experiments?
A: Handle the antibody gently to prevent degradation, avoid repeated freeze-thaw cycles, and protect it from prolonged exposure to light to maintain its functional integrity and performance.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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|---|---|---|---|
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| HPAB-0246-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0246-FY-F(E)) | FC, FuncS, ICC, IHC, IP, WB | Mouse Fab |
| HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
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|---|---|---|---|
| TAB-H46 | Humanized Anti-CD28 Recombinant Antibody (clone Lulizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Humanized V-kappa |
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| TAB-065LC-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (TAB-065LC-F(E)) | ELISA | Humanized Fab |
| TAB-066LC-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (TAB-066LC-F(E)) | ELISA | Humanized Fab |
| TAB-069LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (HuTN228) | FC, ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6) | WB, ELISA, FuncS | Mouse IgG1 |
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| HPAB-0132CQ | Human Anti-CD28 Recombinant Antibody (clone FR255734) | ELISA, IHC, Block, FuncS | Human IgG2M3, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody Fab Fragment (HPAB-1489-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (PABX-031-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (PABX-031-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
| NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
| NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH) | ELISA | Humanized VH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H47) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY9 | Human Anti-CD28 Agonistic Antibody (VS-0724-XY9) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY10 | Mouse Anti-CD28 Agonistic Antibody (VS-0724-XY10) | WB, Agonistic assays | Mouse IgG1 |
| VS-0724-XY11 | Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11) | FC, Agonistic assays, CyTOF® | Rat IgG1 |
| VS-0724-XY12 | Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12) | FC, IF, IHC, Costim, Agonistic assays | Rabbit IgG, kappa |
| VS-0724-XY13 | Mouse Anti-Cd28 Agonistic Antibody (clone JJ316) | FC, IF, IHC, Costim, Agonistic assays | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY52 | AbPlus™ Anti-CD28 Magnetic Beads (VS-0424-XY52) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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