Mouse Anti-Cd28 Recombinant Antibody (clone JJ-319) (CAT#: HPAB-0079-YJ)

This antibody is a mouse antibody that recognizes rat CD28. Mab JJ-319 can be used in researches related to CD28 and may function in the treatment of transplant rejection.


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  • Gene Expression
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  • COA

Figure 1 Costimulation and direct stimulation of primary rat T cells by rat CD28-specific mAb.

Figure 1 Costimulation and direct stimulation of primary rat T cells by rat CD28-specific mAb.

(A) Nylon wool-purified rat lymph node T cells (105/well) were stimulated by anti-TCR mAb R73 immobilized via sheep anti-mouse IgG and soluble rat CD28-specific mAb. An isotype-matched irrelevant mAb served as negative control for the CD28-specific mAb. On day 3, the cells were pulsed with 0.5 μCi [3H]thymidine per well for 16 h followed by harvesting and β counting. (B) As in A but without anti-TCR mAb. One representative experiment from two with the identical outcome is shown.

Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.

FC

Figure 2 Epitope mapping of a superagonistic (JJ316) and conventional (JJ319) rat CD28-specific mAb.

Figure 2 Epitope mapping of a superagonistic (JJ316) and conventional (JJ319) rat CD28-specific mAb.

(A) Comparison of extracellular portions of mouse and rat CD28. Positions of aa differences are indicated by numbers. (B) Binding of superagonistic and conventional mAbs to rat/mouse CD28 chimeras. Mouse sequences are represented in white and rat sequences in black. Positions of swapped aa are indicated on the left. All constructs were stably expressed in L929 fibroblast cells. FACS® histograms are shown for superagonistic (JJ316) and conventional (JJ319) rat and for the conventional mouse CD28-specific mAb 37.51 (solid lines) and an isotype-matched control mAb (dotted lines). At least two independent experiments were performed and a representative experiment is shown.

Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Rat
  • Species Reactivity
  • Rat
  • Clone
  • JJ-319
  • Applications
  • IP, FC
  • Related Disease
  • Transplant Rejection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Flow Cytometry.

Target

  • Alternative Names
  • CD28 Molecule; CD28 Antigen; Tp44; T-Cell-Specific Surface Glycoprotein CD28; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44); JJ-319

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD28"

Chimeric Antibody

CAT Product Name Application Type
TAB-068LC Anti-Human CD28 Recombinant Antibody (ChTN228) ELISA, FC Chimeric antibody (mouse/human)
TAB-068LC-S(P) Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)
TAB-068LC-F(E) Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) FC, ELISA Chimeric antibody (mouse/human)

Mouse Antibody

CAT Product Name Application Type
PABX-031 Recombinant Mouse Anti-CD28 Antibody (5.11A1) WB, ELISA, FuncS, Activ IgG
PABX-031-F (E) Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) WB, ELISA, FuncS Fab

Human Antibody

CAT Product Name Application Type
PABX-031-S (P) Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) WB, ELISA, FuncS scFv

Neutralizing Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-335CQ Hamster Anti-CD28 Recombinant Antibody (clone 37.51) IHC-Fr, FC, IP, Block, FuncS Hamster IgG
NEUT-336CQ Mouse Anti-CD28 Recombinant Antibody (clone E18) FC, Costim, Block Mouse IgG2b, κ

Rabbit Monoclonal Antibody

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H47 Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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