This antibody is a mouse antibody that recognizes rat CD28. Mab JJ-319 can be used in researches related to CD28 and may function in the treatment of transplant rejection.
Figure 1 Costimulation and direct stimulation of primary rat T cells by rat CD28-specific mAb.
(A) Nylon wool-purified rat lymph node T cells (105/well) were stimulated by anti-TCR mAb R73 immobilized via sheep anti-mouse IgG and soluble rat CD28-specific mAb. An isotype-matched irrelevant mAb served as negative control for the CD28-specific mAb. On day 3, the cells were pulsed with 0.5 μCi [3H]thymidine per well for 16 h followed by harvesting and β counting. (B) As in A but without anti-TCR mAb. One representative experiment from two with the identical outcome is shown.
Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.
Figure 2 Epitope mapping of a superagonistic (JJ316) and conventional (JJ319) rat CD28-specific mAb.
(A) Comparison of extracellular portions of mouse and rat CD28. Positions of aa differences are indicated by numbers. (B) Binding of superagonistic and conventional mAbs to rat/mouse CD28 chimeras. Mouse sequences are represented in white and rat sequences in black. Positions of swapped aa are indicated on the left. All constructs were stably expressed in L929 fibroblast cells. FACS® histograms are shown for superagonistic (JJ316) and conventional (JJ319) rat and for the conventional mouse CD28-specific mAb 37.51 (solid lines) and an isotype-matched control mAb (dotted lines). At least two independent experiments were performed and a representative experiment is shown.
Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-981 | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment | Agonist, Stim, Neu, FC | Mouse IgG1 F(ab')2, κ |
PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
There are currently no Customer reviews or questions for HPAB-0079-YJ. Click the button above to contact us or submit your feedback about this product.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.