This antibody is a mouse antibody that recognizes rat CD28. Mab JJ-319 can be used in researches related to CD28 and may function in the treatment of transplant rejection.
Figure 1 Costimulation and direct stimulation of primary rat T cells by rat CD28-specific mAb.
(A) Nylon wool-purified rat lymph node T cells (105/well) were stimulated by anti-TCR mAb R73 immobilized via sheep anti-mouse IgG and soluble rat CD28-specific mAb. An isotype-matched irrelevant mAb served as negative control for the CD28-specific mAb. On day 3, the cells were pulsed with 0.5 μCi [3H]thymidine per well for 16 h followed by harvesting and β counting. (B) As in A but without anti-TCR mAb. One representative experiment from two with the identical outcome is shown.
Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.
Figure 2 Epitope mapping of a superagonistic (JJ316) and conventional (JJ319) rat CD28-specific mAb.
(A) Comparison of extracellular portions of mouse and rat CD28. Positions of aa differences are indicated by numbers. (B) Binding of superagonistic and conventional mAbs to rat/mouse CD28 chimeras. Mouse sequences are represented in white and rat sequences in black. Positions of swapped aa are indicated on the left. All constructs were stably expressed in L929 fibroblast cells. FACS® histograms are shown for superagonistic (JJ316) and conventional (JJ319) rat and for the conventional mouse CD28-specific mAb 37.51 (solid lines) and an isotype-matched control mAb (dotted lines). At least two independent experiments were performed and a representative experiment is shown.
Lühder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Müller, I., Winkler, E.,... & Hünig, T. (2003). Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. The Journal of experimental medicine, 197(8), 955-966.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-981 | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment | Agonist, Stim, Neu, FC | Mouse IgG1 F(ab')2, κ |
FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
HPAB-0246-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0246-FY-F(E)) | FC, FuncS, ICC, IHC, IP, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H46 | Humanized Anti-CD28 Recombinant Antibody (clone Lulizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Humanized V-kappa |
TAB-064LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (TGN1412) | FC | Humanized antibody |
TAB-065LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-065LC-S(P)) | ELISA | Human scFv |
TAB-066LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-066LC-S(P)) | ELISA | Human scFv |
TAB-069LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (HuTN228) | FC, ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
HPAB-0132CQ-S(P) | Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment | ELISA, IHC, Block | Human scFv |
HPAB-0245-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P)) | WB, IP, IF, FC | Mouse scFv |
HPAB-0246-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0246-FY-S(P)) | FC, FuncS, ICC, IHC, IP, WB | Mouse scFv |
HPAB-1589WJ-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (clone 2E12) | ELISA, WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-031 | Recombinant Mouse Anti-CD28 Antibody (5.11A1) | WB, ELISA, FuncS, Activ | IgG |
PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 1) | ELISA | Humanized VH |
FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 2) | ELISA | Humanized VH |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0245-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0245-FY) | WB, IP, IF, FC | Mouse IgG1 |
HPAB-0246-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0246-FY) | FC, FuncS, ICC, IHC, IP, WB | Mouse IgG1 |
FN-086CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.2) | IHC-Fr, FC, IP | Mouse IgG1, κ |
FN-121CQ | Mouse Anti-CD28 Recombinant Antibody (clone 15E8) | FuncS, Activ | Mouse IgG1 |
VS-0723-WK300 | Rabbit Anti-CD28 Recombinant Antibody (VS-0723-WK300) | ELISA, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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