Mouse Anti-CD4 Recombinant Antibody (clone M-T151) (CAT#: FAMAB-0057YC)

Figure 1 Mouse Anti-CD4 Antibody (clone M-T151) in ELISA.

Levels of soluble CD4 in sera from rheumatoid arthritis patients treated with CD4 monoclonal antibody (mAb) M-T151. A sandwich enzyme-linked immunosorbent assay was used to determine soluble CD4 levels, and recombinant soluble CD4 was used as standard. Absorption with immobilized anti-mouse IgG completely removed soluble CD4, indicating that all soluble CD4 was complexed with M-T151. Values are the mean and SD of 14 treatment courses in 10 patients.

Reiter, C., Kakavand, B., Rieber, E. P., Riethmüller, G., Schattenkirchner, M., & Krüger, K. (1991). Treatment of rheumatoid arthritis with monoclonal CD4 antibody M‐T151. Clinical results and immunopharmacologic effects in an open study, including repeated administration. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology, 34(5), 525-536.

Figure 2 Mouse Anti-CD4 Antibody (clone M-T151) in Toxicity.

Toxicity of M-T151-ricin immunotoxins to CEM and Nalm-6 cells exposed to immunotoxin for 1h before incubation. Panel A: CEM cells were incubated in the presence of different concentrations of ricin (○-○), M-T151-bl.ricin (■-■), or M-T151-n.bl.ricin (□-□ ) for 1 h at 4°C, washed once with cold medium, and incubated for 24 h at 37°C before pulsing with [3H] leucine. Panel B: Nalm-6 cells were incubated in the presence of ricin or M-T151-ricin ITs.

Wawrzynczak, E. J., Watson, G. J., Cumber, A. J., Henry, R. V., Parnell, G. D., Rieber, E. P., & Thorpe, P. E. (1991). Blocked and non-blocked ricin immunotoxins against the CD4 antigen exhibit higher cytotoxic potency than a ricin A chain immunotoxin potentiated with ricin B chain or with a ricin B chain immunotoxin. Cancer Immunology, Immunotherapy, 32(5), 289-295.

Figure 3 Mouse Anti-CD4 Antibody (clone M-T151) in Toxicity.

Potentiation of M-T151-ricin-A-chain toxicity to CEM cells by ricin B chain and GAMIg-ricin-B-chain. CEM cells were incubated in the presence of different concentrations of M-T151-ricin-A-chain for 1 h at 4 °C, washed three times with cold medium, then incubated in the presence of medium alone (●-●), ricin B chain (▲-▲) at a concentration of 10 nM, or goat anti-(mouse Ig)-ricin-B-chain (△-△ ) at a concentration of 10 nM with respect to the B chain for 24 h at 37 °C and pulsed with [3H]leucine for a further 24 h. Measurements of [3H]leucine incorporation are expressed as a percentage of incorporation of cultures in medium alone, or with ricin B chain or goat anti-(mouse Ig)-ficin-B-chain as appropriate.

Wawrzynczak, E. J., Watson, G. J., Cumber, A. J., Henry, R. V., Parnell, G. D., Rieber, E. P., & Thorpe, P. E. (1991). Blocked and non-blocked ricin immunotoxins against the CD4 antigen exhibit higher cytotoxic potency than a ricin A chain immunotoxin potentiated with ricin B chain or with a ricin B chain immunotoxin. Cancer Immunology, Immunotherapy, 32(5), 289-295.

Figure 4 Mouse Anti-CD4 Antibody (clone M-T151) in ACTIV.

Activation of human complement by anti-CD4 mAb. Deposition of complement components C3c, C4 and C5 is shown as total fluorescence mean channel after detection by C3-, C4- and C5-specific FITC-conjugated antisera in a FACscan analysis; a, m M-T151 (IgG2a); b, m M-T310 (IgG1); c,m M-T151+m M-T310; d,chim M-T151 (IgG1); e, chim M-T310 (IgG1); f, chim M-T151+chim M-T310.

Weissenhorn, W., Scheuer, W., Kaluza, B., Schwirzke, M., Reiter, C., Flieger, D., ... & Weidle, U. H. (1992). Combinatorial functions of two chimeric antibodies directed to human CD4 and one directed to the α-chain of the human interleukin-2 receptor. Gene, 121(2), 271-278.

Figure 5 Mouse Anti-CD4 Antibody (clone M-T151) in Northern blot.

Northern blot analysis of hybridomas and transfectomas secreting murine and chimeric mAb M-T151 and M-T310.

Weissenhorn, W., Scheuer, W., Kaluza, B., Schwirzke, M., Reiter, C., Flieger, D., ... & Weidle, U. H. (1992). Combinatorial functions of two chimeric antibodies directed to human CD4 and one directed to the α-chain of the human interleukin-2 receptor. Gene, 121(2), 271-278.