CytoStream™ Rat Anti-CDH5 Recombinant Antibody (VS-0225-XY83)
CAT#: VS-0225-XY83
Targeting CDH5, a key component in endothelial cell adhesion, this rat-derived antibody exhibits strong reactivity with mouse samples. Designed for a range of applications, it excels in flow cytometry, blocking assays, immunohistochemistry, and immunoprecipitation. The antibody's precise targeting of CDH5 enables detailed investigation of endothelial junctions and vascular dynamics. With its robust performance across multiple platforms, it supports cutting-edge research in vascular biology and endothelial function, offering exceptional sensitivity and specificity in cellular studies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear membrane & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75875/1842_A1_68_selected.jpg
Normal Tissue
Figure 2 IHC staining of human vagina
Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in superficial layers of squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28366/66242_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000179776-CDH5
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Specifications
- Immunogen
- Mouse VE-Cadherin-Ig Fusion
- Host Species
- Rat
- Type
- Rat IgG2a, kappa
- Specificity
- CDH5
- Species Reactivity
- Mouse
- Applications
- FC, Block, IHC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDH5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1657z | Mouse Anti-CDH5 Recombinant Antibody (clone 24B9) | WB, FC, ICC, IF, IHC, IP, FuncS | Mouse IgG2a |
| ZG-0169C | Mouse Anti-CDH5 Recombinant Antibody (clone 3G8) | IHC-P, IF | Mouse IgG |
| ZG-0170C | Mouse Anti-CDH5 Recombinant Antibody (clone 8E5) | IHC-P, IF | Mouse IgG |
| VS3-XY472 | Mouse Anti-CDH5 Recombinant Antibody (clone 4F12) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY473 | Mouse Anti-CDH5 Recombinant Antibody (clone 5H4) | ELISA, WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-283CQ | Anti-Mouse Cdh5 Recombinant Antibody (E4B9) | FuncS | |
| TAB-283CQ-S(P) | Anti-Mouse Cdh5 Recombinant Antibody scFv Fragment (E4B9) | ELISA, WB, IF | |
| TAB-283CQ-F(E) | Anti-Mouse Cdh5 Recombinant Antibody Fab Fragment (E4B9) | ELISA, WB, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-449CQ | Mouse Anti-CDH5 Recombinant Antibody (VMC176) | Inhib, WB, IP | Mouse IgG2a |
| NEUT-450CQ | Rat Anti-Cdh5 Recombinant Antibody (clone BV13) | FC, WB, IF, Block | Rat IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4075 | Hi-Affi™ Rabbit Anti-CDH5 Recombinant Antibody (clone SI142DS) | IHC-P | Rabbit IgG |
| MOR-4076 | Hi-Affi™ Rabbit Anti-CDH5 Recombinant Antibody (clone SI143DS) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1016LC | Mouse Anti-Cdh5 Recombinant Antibody (clone 10G4) | FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC194 | CytoStream™ Rabbit Anti-CDH5 Recombinant Antibody (VS13-YC194) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC240 | Recombinant Anti-CDH5 Vesicular Antibody, EV Displayed (VS-0425-YC240) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1298 | Anti-CDH5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1299 | Anti-Mouse CDH5 Immunohistochemistry Kit | IHC |
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