Mouse Anti-H3C1/H3C2 Recombinant Antibody (clone 0725W14)
CAT#: VS-0725-FY169
The Mouse Anti-H3C1/H3C2 Recombinant Antibody (clone 0725W14) is a recombinant antibody targeting amino acids 21-39 of human Histone H3.1. It is validated for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). As a core component of the nucleosome, histones play central roles in transcriptional regulation, DNA repair, DNA replication, and chromosomal stability. This antibody is a useful tool for studying the fundamental processes governed by histone modifications.
Specifications
- Immunogen
- A peptide comprising amino acids 21-39 of human Histone H3.1.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human H3C1/H3C2
- Species Reactivity
- Human
- Clone
- 0725W14
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Glioma Susceptibility 1, Plasma Cell Neoplasm
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunocytochemistry/Immunofluorescence: 0.4-2 µg/mL
Western Blot: 0.2-2 µg/mL
Target
- Alternative Names
- H3 histone, HIST1H3A, Histone cluster 1, H3a
- Gene ID
- 8290
- UniProt ID
- P68431
- Long Name
- Histone H3
- Cellular Localization
- Nucleus, Chromosome
- Post Translation Modifications
- Glycosylation at Ser11 (NX_P68431 [NX_P68431-1])
Ubiquitination at Lys116 (NX_P68431 [NX_P68431-1])
Modification
Glycosylation from GlyConnect H31_HUMAN (2886)
Glycosylation from GlyGen (P68431) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- NP_003520.1
- Function
- Core component of nucleosome.
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY169. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, Inhib, FC, Neut
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: IF, IP, Neut, FuncS, ELISA, FC
-2.png)
Application: WB, ELISA, FuncS
Application: ELISA, Neut, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
