Provided is a recombinant human antibody that recognizes HAVCR2 at the position between amino acid residues 62-67. This antibody has an affinity of about 25 nanomolar for the human TIM-3 ectodomain.
Figure 1 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in WB
Western blot analysis was performed by loading 5 µg (lane 1) and 2 µg (lane 2) recombinant human HAVCR2 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-HAVCR2 Antibody (HPAB-M0674-YC) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Figure 2 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in ELISA
ELISA analysis of HPAB-M0674-YC was performed by coating with recombinant human HAVCR2 Protein. Then blocked with BSA, and incubated with Anti-HAVCR2 Antibody (HPAB-M0674-YC) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-893CQ | Rat Anti-Havcr2 Recombinant Antibody (clone B8.2C12) | Block, FC | Rat IgG1 |
NEUT-894CQ | Rat Anti-Havcr2 Recombinant Antibody (clone RMT3-23) | Block, FC | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4214 | Rabbit Anti-HAVCR2 Recombinant Antibody (clone SI293DS) | ELISA | Rabbit IgG |
MOR-4215 | Rabbit Anti-HAVCR2 Recombinant Antibody (clone SI294DS) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0507-CN | Mouse Anti-HAVCR2 Recombinant Antibody (HPAB-0507-CN) | ELISA, FC | Mouse IgG |
HPAB-0508-CN | Mouse Anti-HAVCR2 Recombinant Antibody (HPAB-0508-CN) | ELISA, FC | Mouse IgG |
HPAB-0509-CN | Human Anti-HAVCR2 Recombinant Antibody (HPAB-0509-CN) | ELISA, FC | Chimeric (mouse/human) IgG1, κ |
HPAB-0510-CN | Human Anti-HAVCR2 Recombinant Antibody (HPAB-0510-CN) | ELISA, FC | Humanized IgG1, κ |
HPAB-0511-CN | Mouse Anti-HAVCR2 Recombinant Antibody (HPAB-0511-CN) | ELISA, FC, IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0506-CN-F(E) | Mouse Anti-HAVCR2 Recombinant Antibody; Fab Fragment (HPAB-0506-CN-F(E)) | ELISA, FC | Mouse Fab |
HPAB-0507-CN-F(E) | Mouse Anti-HAVCR2 Recombinant Antibody; Fab Fragment (HPAB-0507-CN-F(E)) | ELISA, FC | Mouse Fab |
HPAB-0394-YC-F(E) | Mouse Anti-HAVCR2 Recombinant Antibody; Fab Fragment (HPAB-0394-YC-F(E)) | FC, ELISA | Mouse Fab |
HPAB-0395-YC-F(E) | Human Anti-HAVCR2 Recombinant Antibody; Fab Fragment (HPAB-0395-YC-F(E)) | FC, ELISA | Humanized Fab |
HPAB-0398-YC-F(E) | Mouse Anti-HAVCR2 Recombinant Antibody; Fab Fragment (HPAB-0398-YC-F(E)) | ELISA, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0846-WJ-S(P) | Human Anti-HAVCR2 Recombinant Antibody (clone L3D); scFv Fragment | Neut, ELISA | Humanized scFv |
HPAB-0453-FY-S(P) | Human Anti-HAVCR2 Recombinant Antibody; scFv Fragment (HPAB-0453-FY-S(P)) | ELISA | Human scFv |
HPAB-0454-FY-S(P) | Human Anti-HAVCR2 Recombinant Antibody; scFv Fragment (HPAB-0454-FY-S(P)) | ELISA | Human scFv |
HPAB-0455-FY-S(P) | Mouse Anti-HAVCR2 Recombinant Antibody (clone Tim3_0022); scFv Fragment | FC | Mouse scFv |
HPAB-0681YY-S(P) | Human Anti-HAVCR2 Recombinant Antibody; scFv Fragment (HPAB-0681YY-S(P)) | ELISA, FuncS | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
VS3-WK1520 | Human Anti-HAVCR2 Recombinant Antibody (clone TSR-022) | ELISA, FC | Humanized IgG4 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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