Human Anti-HAVCR2 Recombinant Antibody (HPAB-M0674-YC) (CAT#: HPAB-M0674-YC)

Provided is a recombinant human antibody that recognizes HAVCR2 at the position between amino acid residues 62-67. This antibody has an affinity of about 25 nanomolar for the human TIM-3 ectodomain.


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WB

Figure 1 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in WB

Figure 1 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in WB

Western blot analysis was performed by loading 5 µg (lane 1) and 2 µg (lane 2) recombinant human HAVCR2 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-HAVCR2 Antibody (HPAB-M0674-YC) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

ELISA

Figure 2 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in ELISA

Figure 2 Anti-HAVCR2 Antibody (HPAB-M0674-YC) in ELISA

ELISA analysis of HPAB-M0674-YC was performed by coating with recombinant human HAVCR2 Protein. Then blocked with BSA, and incubated with Anti-HAVCR2 Antibody (HPAB-M0674-YC) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Type
  • Human lgG1
  • Specificity
  • Human HAVCR2
  • Species Reactivity
  • Human
  • Applications
  • Block, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Hepatitis A Virus Cellular Receptor 2; T-Cell Immunoglobulin And Mucin Domain-Containing Protein 3; T-Cell Immunoglobulin Mucin Family Member 3; T-Cell Immunoglobulin Mucin Receptor 3; T-Cell Membrane Protein 3; HAVcr-2; TIMD-3; Tim-3;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HAVCR2"

Blocking Antibody

Rabbit Monoclonal Antibody

Humanized Antibody

CAT Product Name Application Type
VS3-WK1520 Human Anti-HAVCR2 Recombinant Antibody (clone TSR-022) ELISA, FC Humanized IgG4

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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