Human Anti-HSPA5 Recombinant Antibody (clone huMAb159) (CAT#: HPAB-0369-YJ)
This product is a recombinant humanized antibody that recognizes human HSPA5. It cross-reacted with mouse HSPA5. It may be useful for UPR-targeted cancer therapeutic treatments.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Figure 2 Colon
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Figure 3 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Figure 7 Thyroid gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
Specifications
- Immunogen
- Human GRP78 (amino acids 1-650; without KDEL motif) and with hexahistidine tag on C-terminus (designated as GRP78-His) was constructed, transiently expressed in 293T cells, purified through nickel-NTA column and used as an immunogen.
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse
- Clone
- huMAb159
- Applications
- WB, FC, IHC, ELISA, Inhib
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Flow Cytometry, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Inhibition.
Target
- Alternative Names
- BIP; MIF2; GRP78; HEL-S-89n; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; HSP70 family protein 5; binding-immunoglobulin protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 70 family protein 5; heat shock protein family A member 5; immunoglobulin heavy chain-binding protein
- Gene ID
- 3309
- UniProt ID
- P11021
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibody Products
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ScFv Antibody Products
Fab Antibody Products
Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
| TAB-009CT-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (TAB-009CT-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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