Mouse Anti-NFKB1 Monoclonal Antibody (clone 5D10D11)
CAT#: VS7-0425-WR474
This product is a mouse IgG2a antibody that binds to human NFKB1. The antibody 5D10D11 can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27305/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_8.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_3.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109320-NFKB1
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Specifications
- Immunogen
- Recombinant human NFKB1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human NFKB1
- Species Reactivity
- Human
- Clone
- 5D10D11
- Molecule Class
- NF-κB Transcription Factor
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, common variable, 12, with autoimmunity (CVID12)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta
- Gene ID
- 4790
- UniProt ID
- P19838
- Long Name
- Nuclear Factor Kappa B Subunit 1
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Hydroxylation; Isopeptide bond; Lipoprotein; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_001158884.1
- Function
- Activator; DNA-binding
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NFKB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1566z | Mouse Anti-NFKB1 Recombinant Antibody (clone 31H12) | WB, IF, IHC | Mouse IgG1 |
| MOB-1711MZ | Recombinant Mouse Anti-Human NFKB1 Antibody | IHC-P, WB | Mouse antibody |
| MRO-2071-CN | Rabbit Anti-NFKB1 Polyclonal Antibody (MRO-2071-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ754 | Rabbit Anti-NFKB1 Recombinant Antibody (VS3-CJ754) | WB, IHC | Rabbit IgG |
| VS3-FY2819 | Mouse Anti-NFKB1 Recombinant Antibody (VS3-FY2819) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0391MZ | Chicken Anti-NFKB1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2433 | Hi-Affi™ Recombinant Rabbit Anti-NFKB1 Monoclonal Antibody (DS2433AB) | WB, IHC-P, ICC, FC | IgG |
| MOR-4511 | Hi-Affi™ Recombinant Rabbit Anti-NFKB1 Monoclonal Antibody (TH19DS) | WB, FC | IgG |
| MOR-0096-FY | Rabbit Anti-NFKB1 Recombinant Antibody (clone AFY0067) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1090-CN | Recombinant Mouse Anti-NFKB1 Monoclonal Antibody (A2-7) | WB, IF, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4817 | Anti-NFKB1 Immunohistochemistry Kit | IHC |
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