Mouse Anti-NFKB1 Monoclonal Antibody (clone 5D10D11) (CAT#: VS7-0425-WR474)
This product is a mouse IgG2a antibody that binds to human NFKB1. The antibody 5D10D11 can be used for WB, IHC, ICC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27305/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27305/if_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_8.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_3.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109320-NFKB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109320-NFKB1
Specifications
- Immunogen
- Recombinant human NFKB1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human NFKB1
- Species Reactivity
- Human
- Clone
- 5D10D11
- Molecule Class
- NF-κB Transcription Factor
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, common variable, 12, with autoimmunity (CVID12)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta
- Gene ID
- 4790
- UniProt ID
- P19838
- Long Name
- Nuclear Factor Kappa B Subunit 1
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Hydroxylation; Isopeptide bond; Lipoprotein; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_001158884.1
- Function
- Activator; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NFKB1"
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IgG Antibody Products
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Rabbit Monoclonal Antibody Products
IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1566z | Mouse Anti-NFKB1 Recombinant Antibody (clone 31H12) | WB, IF, IHC | Mouse IgG1 |
| MOB-1711MZ | Recombinant Mouse Anti-Human NFKB1 Antibody | IHC-P, WB | Mouse antibody |
| VS7-0425-WR473 | Mouse Anti-NFKB1 Monoclonal Antibody (clone 5D10) | WB, IHC, FC | Mouse IgG2a |
| VS7-0425-WR475 | Mouse Anti-NFKB1 Monoclonal Antibody (VS7-0425-WR475) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR476 | Mouse Anti-NFKB1 Monoclonal Antibody (VS7-0425-WR476) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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