Mouse Anti-NHP RAD23B Recombinant Antibody (clone 5H1) (CAT#: VS-1024-XY430)
Focusing on RAD23B, this mouse monoclonal antibody showcases reactivity with human, mouse, non-human primate, and rat tissues. The immunogen comprises purified recombinant human RAD23B protein fragments, ensuring high specificity and reliable binding. It is well-suited for various applications, including Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), making it an invaluable asset for investigating RAD23B expression and its biological roles across multiple experimental platforms, thereby enhancing research capabilities in cellular and molecular studies.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/296_F12_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/296_F12_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/if_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_7_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_5.jpg
Figure 8 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_4_6.jpg
Figure 9 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_9_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119318-RAD23B
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119318-RAD23B
Specifications
- Immunogen
- Purified recombinant human RAD23B protein fragments
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- RAD23B
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 5H1
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:50-1:200, IF: 1:50-1:200
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| MOB-0901CT | Recombinant Mouse anti-Human RAD23B Monoclonal antibody (3968E8b) | Dot, IHC-P, WB | |
| FAMAB-1236CQ | Mouse Anti-RAD23B Recombinant Antibody (clone SAIC-28B-8) | Immuno-MRM, ELISA | Mouse IgG1 |
| FAMAB-1237CQ | Mouse Anti-RAD23B Recombinant Antibody (clone SAIC-28A-29) | Immuno-MRM, WB | Mouse IgG1 |
| ZG-0429J | Mouse Anti-RAD23B Recombinant Antibody (ZG-0429J) | WB, ICC | Mouse IgG |
| VS3-FY1240 | Recombinant Mouse Anti-RAD23B Antibody (clone 6D2B3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1, κ |
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