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Mouse Anti-NHP RAD23B Recombinant Antibody (clone 5H1) (CAT#: VS-1024-XY430)

Focusing on RAD23B, this mouse monoclonal antibody showcases reactivity with human, mouse, non-human primate, and rat tissues. The immunogen comprises purified recombinant human RAD23B protein fragments, ensuring high specificity and reliable binding. It is well-suited for various applications, including Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), making it an invaluable asset for investigating RAD23B expression and its biological roles across multiple experimental platforms, thereby enhancing research capabilities in cellular and molecular studies.

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  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/296_F12_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33868/ihc_selected.jpg
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_B_8_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_3.jpg
Figure 6 Liver
Figure 6 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_7_4.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_4_6.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29718/67879_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119318-RAD23B

Specifications

  • Immunogen
  • Purified recombinant human RAD23B protein fragments
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • RAD23B
  • Species Reactivity
  • Human, Mouse, Non-human primate, Rat
  • Clone
  • 5H1
  • Applications
  • WB, IHC, IF

Product Property

  • Purification
  • Protein A purified
  • Purity
  • >95%, determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • PBS, pH 7.4, with 50% glycerol
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • WB: 1:800, IHC: 1:50-1:200, IF: 1:50-1:200

Target

  • Alternative Names
  • P58; HR23B; HHR23B

Recommended Products

Recommended Dilution Buffer

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RAD23B"

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CAT Product Name Application Type
MOB-0901CT Recombinant Mouse anti-Human RAD23B Monoclonal antibody (3968E8b) Dot, IHC-P, WB
FAMAB-1236CQ Mouse Anti-RAD23B Recombinant Antibody (clone SAIC-28B-8) Immuno-MRM, ELISA Mouse IgG1
ZG-0429J Mouse Anti-RAD23B Recombinant Antibody (ZG-0429J) WB, ICC Mouse IgG
VS3-CJ1040 Mouse Anti-RAD23B Recombinant Antibody (VS3-CJ1040) WB Mouse IgG1, κ
VS3-FY1240 Recombinant Mouse Anti-RAD23B Antibody (clone 6D2B3) ELISA, WB, IHC, ICC, FC Mouse IgG1, κ

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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