Rabbit Anti-PCSK9 Recombinant Antibody (clone 7F2) (CAT#: ZG-0502U)
Rabbit Anti-PCSK9 Antibody (clone 7F2) displayed high specificity against the PCSK9 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PCSK9 Antibody (ZG-0502U) in WB
Western Blot
Positive WB detected in: PC-3 whole cell lysate
All lanes: PCSK9 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 75, 21 kDa
Observed band size: 60, 75 kDa
IHC
Figure 2 Rabbit Anti-PCSK9 Antibody (ZG-0502U) in IHC
IHC image of ZG-0502U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PCSK9
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PCSK9
- Species Reactivity
- Human
- Clone
- 7F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hypercholesterolemia, Familial, 3 and Homozygous Familial Hypercholesterolemia.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Proprotein convertase subtilisin/kexin type 9; EC 3.4.21.-; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9; PCSK9; NARC1
- Gene ID
- 255738
- UniProt ID
- Q8NBP7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H31 | Human Anti-PCSK9 Recombinant Antibody (TAB-H31) | FC, IP, ELISA, Neut, FuncS, IF, IHC | Human IgG2, λ |
| TAB-0953CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0953CL-F(E)) | ELISA | Human Fab |
| TAB-0954CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0954CL-F(E)) | WB | Human Fab |
| TAB-0955CL-F(E) | Mouse Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0955CL-F(E)) | WB | Mouse Fab |
| TAB-0959CL-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (TAB-0959CL-F(E)) | ELISA, FC | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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