Mouse Anti-PDHA1 Recombinant Antibody (VS7-0425-WR586)
CAT#: VS7-0425-WR586
This product is a mouse IgG antibody that binds to human PDHA1. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, rat, bovine, dog, and pig.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/1645_G11_19_cr57b568f223f4d_selected.jpg
Normal Tissue
Figure 2 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_B_5_6.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47487/113196_A_9_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47864/113115_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131828-PDHA1
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Specifications
- Immunogen
- Recombinant human PDHA1 (C-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PDHA1
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog, Pig
- Molecule Class
- Pyruvate Dehydrogenase
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
- Related Disease
- Pyruvate dehydrogenase E1-alpha deficiency (PDHAD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- Pyruvate Dehydrogenase E1 Alpha 1 Subunit; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Somatic Form, Mitochondrial; Pyruvate Dehydrogenase (Lipoamide) Alpha 1; Pyruvate Dehydrogenase Alpha 1; PDHE1-A Type I; EC 1.2.4.1; PHE1A
- Gene ID
- 5160
- UniProt ID
- P08559
- Long Name
- Pyruvate Dehydrogenase E1 Alpha 1 Subunit
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000275.1
- Function
- Oxidoreductase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PDHA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2639 | Hi-Affi™ Recombinant Rabbit Anti-PDHA1 Monoclonal Antibody (DS2639AB) | WB, IHC-P, ICC, IF, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4342 | PE-A*01:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4343 | PE-A*03:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4344 | PE-A*11:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM | |
| MHC-LC4345 | PE-A*69:01/Human PDHA1 (KSDPIMLLK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-041R | Mouse Anti-PDHA1 Recombinant Antibody (ZG-041R) | WB, ICC | Mouse IgG |
| VS3-WK441 | Rabbit Anti-PDHA1 Recombinant Antibody (clone JF996-0) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1115 | Recombinant Mouse Anti-PDHA1 Antibody (clone 1G10C10) | ELISA, WB, IP, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY1116 | Recombinant Mouse Anti-PDHA1 Antibody (clone 2A2H7) | ELISA, WB, FC | Mouse IgG2b |
| VS3-FY1117 | Recombinant Mouse Anti-PDHA1 Antibody (clone 4E12D6) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY372 | Rabbit Anti-NHP PDHA1 Recombinant Antibody (VS-1024-XY372) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1601 | Anti-PDHA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5281 | Anti-Mouse PDHA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5280 | Anti-Human PDHA1 Immunohistochemistry Kit | IHC |
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