Rabbit Anti-PKM Recombinant Antibody (clone R09-7E7) (CAT#: VS3-XY3466)
It is a recombinant rabbit antibody binding to PKM (Pyruvate kinase M1/2), which catalyzes the final rate-limiting step of glycolysis. The antibody specifically reacts with human, mouse and rat PKM. This antibody has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
Figure 3 IHC staining of human fallopian tube
(Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
(Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
Figure 4 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PKM
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-7E7
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Pyruvate Kinase Deficiency Of Red Cells; Anterior Dislocation Of Lens
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
- Gene ID
- 5315
- UniProt ID
- P14618
- Long Name
- Pyruvate kinase M1/2
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- ISGylated. (P14618-KPYM_HUMAN)
Under hypoxia, hydroxylated by EGLN3. (P14618-KPYM_HUMAN)
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
- Protein Refseq
- XP_005254502.1; XP_005254500.1
- Function
- Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed:20847263, 15996096, 1854723).
The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed:20847263, 15996096, 1854723).
The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0378C | Mouse Anti-PKM Recombinant Antibody (ZG-0378C) | WB, ICC | Mouse IgG |
| ZG-0379C | Mouse Anti-PKM Recombinant Antibody (ZG-0379C) | WB, ICC | Mouse IgG |
| ZG-0247U | Mouse Anti-PKM Recombinant Antibody (clone 6C3C7) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG1 |
| ZG-0248U | Mouse Anti-PKM Recombinant Antibody (clone 4C8E12) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG1 |
| ZG-0513U | Rabbit Anti-PKM Recombinant Antibody (clone 7B2) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
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