Rabbit Anti-PTBP1 Recombinant Antibody (clone 8A1) (CAT#: ZG-0526U)
Rabbit Anti-PTBP1 Antibody (clone 8A1) displayed high specificity against the PTBP1 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-PTBP1 Antibody (ZG-0526U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: PTBP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 60, 60 kDa
Observed band size: 58 kDa
IHC
Figure 2 Rabbit Anti-PTBP1 Antibody (ZG-0526U) in IHC
IHC image of ZG-0526U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PTBP1 Antibody (ZG-0526U) in IHC
IHC image of ZG-0526U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PTBP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTBP1
- Species Reactivity
- Human
- Clone
- 8A1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Human T-Cell Leukemia Virus Type 2 and Patellar Tendinitis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Polypyrimidine tract-binding protein 1; PTB; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I; PTBP1; PTB
- Gene ID
- 5725
- UniProt ID
- P26599
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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|---|---|---|---|
| MOB-1658CT | Recombinant Mouse anti-Human PTBP1 Monoclonal antibody (EML2203) | IHC-P, WB | |
| MRO-1290-CN | Recombinant Mouse Anti-PTBP1 Monoclonal Antibody (9A1) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-1291-CN | Recombinant Mouse Anti-PTBP1 Monoclonal Antibody (5C1) | WB, IHC | Mouse IgG1 |
| MRO-1292-CN | Recombinant Rabbit Anti-PTBP1 Monoclonal Antibody (JU41-20) | WB, IHC | Rabbit IgG |
| VS3-FY1210 | Recombinant Rabbit Anti-PTBP1 Antibody (clone R01-1G2) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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