Mouse Anti-RNA pol II CTD phospho Ser2 Recombinant Antibody (clone 0725W59)
CAT#: VS-0725-FY226
This recombinant antibody, clone 0725W59, targets a peptide containing the RNA Pol II CTD sequence, specifically phosphorylated at serine 2. It is validated for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Enzyme-linked Immunosorbent Assay (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). RNA polymerase II, with its critical CTD repeats, is the key enzyme for mRNA synthesis in eukaryotes. This antibody is an important tool for studying transcriptional activity.
Specifications
- Immunogen
- A peptide containing the RNA Pol II CTD sequence, specifically phosphorylated at serine 2.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human RNA pol II CTD phospho Ser2
- Species Reactivity
- Human
- Clone
- 0725W59
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 240 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µg each
Immunocytochemistry/Immunofluorescence: 1-2 µg/mL
Western Blot: 0.5-2 µg/mL
Bead-based Enzyme-linked Immunosorbent Assay: 0.1-5 µg/mL
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA polymerase II C-terminal domain phosphorylated at Serine 2
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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