Rabbit Anti-VDAC1 Recombinant Antibody (VS3-CJ780)
CAT#: VS3-CJ780
This product is a rabbit antibody that recognizes human, mouse, and rat VDAC1.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_A_7_8.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_A_7_5.jpg
Normal Tissue
Figure 6 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30780/155841_B_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213585-VDAC1
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Specifications
- Immunogen
- Recombinant protein of human VDAC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat VDAC1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunofluorescence (1:50-1:200), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- PORIN; VDAC-1
- Gene ID
- 7416
- UniProt ID
- P21796
- Sequence Similarities
- Belongs to the eukaryotic mitochondrial porin family.
- Cellular Localization
- Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.
Ubiquitinated (PubMed:25621951, PubMed:32047033).
Undergoes monoubiquitination and polyubiquitination by PRKN; monoubiquitination at Lys-274 inhibits apoptosis, whereas polyubiquitination leads to its degradation and promotes mitophagy (PubMed:25621951, PubMed:32047033).
Deubiquitinated by USP30 (PubMed:25621951).
- Protein Refseq
- NP_003365.1
- Function
- Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959).
Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432).
In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033).
May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).
May mediate ATP export from cells (PubMed:30061676).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "VDAC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1720z | Mouse Anti-VDAC1 Recombinant Antibody (clone 37D1) | WB, ICC, IF, IHC | Mouse IgG2a |
| MOB-0210MC | Wheat Anti-VDAC1 Recombinant Antibody (clone YC177) | AP, ELISA, WB | Wheat IgG |
| MOB-0211MC | Wheat Anti-VDAC1 Recombinant Antibody (clone YC178) | AP, ELISA, WB | Wheat IgG |
| MOB-0212MC | Wheat Anti-VDAC1 Recombinant Antibody (clone YC179) | AP, ELISA, WB | Wheat IgG |
| VS3-FY1542 | Recombinant Rabbit Anti-VDAC1 Antibody (clone R05-7F2) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3833 | Rabbit Anti-VDAC1 Recombinant Antibody (clone DS3833AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC488 | AbPlus™ Anti-VDAC1 Magnetic Beads (VS-0724-YC488) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2465 | Anti-VDAC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7830 | Anti-Mouse VDAC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7829 | Anti-Human VDAC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7831 | Anti-Rat VDAC1 Immunohistochemistry Kit | IHC |
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