Rabbit Anti-VIM (pS72) Recombinant Antibody (clone R04-1C8)

Name: Rabbit Anti-VIM (pS72) Recombinant Antibody (clone R04-1C8)
SKU: VS3-WK2018
Rating: 5 (1 reviews)

CAT#: VS3-WK2018

This product is a rabbit recombinant antibody that is specific to phosphorylated VIM. The target of this antibody is phospho-VIM (Ser72). The clone R04-1C8 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of VIM (pSer72) observed in WB experiments using this antibody is 54 kDa.

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line ASC TERT1

Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%

Fibroblasts
Staining:

High

Intensity: Strong

Mucosal lymphoid cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg

Normal Tissue Figure 6 Kidney

Cells in glomeruli
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg

Normal Tissue Figure 7 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Sertoli cells
Staining:

High

Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg

Normal Tissue Figure 8 Breast

Adipocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

Myoepithelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

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Figure 1 Rabbit Anti-VIM (pSer72) Recombinant Antibody (clone R04-1C8) in WB.

Western blot analysis of Vimentin (phospho-Ser72) in rat brain lysates using anti-VIM (pSer72) antibody, Cat# VS3-WK2018.

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Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues Ser72 of human Vimentin

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Phospho-Ser72 of human and rat VIM

Species Reactivity
Human, Rat

Clone
R04-1C8

Applications
WB, IP

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IP: 1/20

Target

Alternative Names
Vimentin

Gene ID
7431

UniProt ID
P08670

Long Name
Vimentin

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK2018.pdf

MSDS

MSDS - VS3-WK2018.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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