Human Anti-ACE2 (clone 05B04) scFv-Fc Chimera (CAT#: VS-0425-FY8)

• Immunogen
• Recombinant hACE2 extracellular domain (1-740 aa).

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Specificity
• Human ACE2

• Species Reactivity
• Human

• Clone
• 05B04

• Applications
• Flow Cytometry, Neutralization, Immunofluorescence, ELISA

• Related Disease
• Covid-19 and Coronavirus Infectious Disease

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Flow Cytometry: 2 μg/mL, Neutralization: 1 μg/mL
  The optimal dilution concentration is determined by the experiment.

• Alternative Names
• Angiotensin I Converting Enzyme 2; Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2; Angiotensin-Converting Enzyme Homolog; ACE-Related Carboxypeptidase; Metalloprotease MPROT15; Peptidyl-Dipeptidase A

• Gene ID
• 59272

• UniProt ID
• Q9BYF1

• Long Name
• Angiotensin I Converting Enzyme 2

• Sequence Similarities
• Belongs to the peptidase M2 family.

• Cellular Localization
• Cell membrane; Single-pass type I membrane protein; Cytoplasm; Cell projection, cilium; Apical cell membrane

• Post Translation Modifications
• N-glycosylation on Asn-90 may limit SARS infectivity.
  Proteolytic cleavage by ADAM17 generates a secreted form.
  Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.
  Phosphorylated.
  Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains.
  Ubiquitinated.
  Ubiquitinated on Lys-788 via 'Lys-48'-linked ubiquitin.
  'Lys-48'-linked deubiquitinated by USP50 on the Lys-788; leading to its stabilization.
  Glycosylation at Asn53, Asn432, Asn90, Asn103, Asn546, Asn322, Asn690, and Thr730
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyConnect ACE2_HUMAN (1012)
  Glycosylation from GlyGen 19 sites, 304 N-linked glycans (8 sites), 35 O-linked glycans (11 sites)

• Protein Refseq
• NP_001358344.1; NP_001373188.1; NP_001373189.1; NP_001375381.1; NP_001376331.1

• Function
• Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis.
  Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II.
  Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin.

• VS-0624-FY1
• Human Anti-ACE2 Recombinant Antibody (clone 05B04)

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