Magic™ Sugar-inducible Bacillus subtilis Expression Service

The MagicTM Sugar-inducible Bacillus subtilis Expression Service of Creative Biolabs offers an incredible expression of Bacillus subtilis for numerous conventional or novel discovered functional biopharmaceuticals, such as varied antibody formats of conventional antibody, heavy chain antibody, single chain antibody, intrabody, etc., vaccines for numerous pathogens of human or other animals, and natural or designed peptides with particular bio-functions of anti-microbial, anti-virus, anti-venom, therapeutic effect, etc. Combining the D-mannose induction and high-cell-density fermentation, our platform provides the most superior sugar-inducible expression services of B. subtilis to satisfy the most demanding product in the world.

The MagicTM Sugar-inducible Bacillus subtilis Expression Service of Creative Biolabs offers an incredible expression of Bacillus subtilis for numerous conventional or novel discovered functional biopharmaceuticals, such as varied antibody formats of conventional antibody, heavy chain antibody, single chain antibody, intrabody, etc., vaccines for numerous pathogens of human or other animals, and natural or designed peptides with particular bio-functions of anti-microbial, anti-virus, anti-venom, therapeutic effect, etc. Combining the D-mannose induction and high-cell-density fermentation, our platform provides the most superior sugar-inducible expression services of B. subtilis to satisfy the most demanding product in the world.

The Introduction and Application of Sugar-inducible Bacillus subtilis Expression

Once the creative manipulation of sugar-inducible B. subtilis expression system was developed, our scientists at Creative Biolabs swiftly studied this excellent technique thoroughly in every detail. The current sugar-inducible mechanism is mainly based on the manR operon (PmanO) and mannose operon. Although the latter operon takes the major responsibility for the induction and subsequent expression (PmanO), the former gene irreplaceably codes for a putative activator of the latter. Both cassettes are capable of being induced efficiently by D-mannose. This sugar monomer is a C-2 epimer of glucose and belongs to the aldohexose series of carbohydrates, which is widely existed in numerous biomolecules including the mannan, heteromannan polysaccharides, glycoproteins, and other glycoconjugates. Function as the specific and efficient inducer of both operons, D-mannose is transported by the manP gene of the mannose operon, which is recognized as a putative PTS mannose-specific enzyme IIBCA component and mannose transporter. Further research revealed a constitutive activation of both operon once the manP gene was deleted, which laid down the mechanical foundation of the MagicTM Auto-inducible Bacillus subtilis Expression Service. Another gene manA is responsible for the subsequent metabolic reaction by coding a mannose-6-phosphate isomerase. As for the third gene of the mannose operon, the yjdF manifests a mysterious biofunction waiting for discovery.

Advantages of MagicTM Sugar-inducible Bacillus subtilis Expression Service

1. Extremely high success rate for almost a hundred of proteins and antibodies for the past decade of service.
2. Multiple excellent strains and vector systems for regular and toxic products via intracellular accumulation or secretion expression.
3. Optimal cultivation and induction at Creative Biolabs with the whole range of scales from 5 mL to 1000 L per batch.
4. One-stop service: from initial design and optimization all the way down to purification and further modification with well recorded data and organized report.

The original D-mannose-inducible expression of B. subtilis leads to a 4- to 7-fold increase of the EGFP expression with an optimal concentration of D-mannose. In addition to the manipulation of the carbon catabolite repression (CCR) and high-cell-density fermentation, the advanced expression system elevated the protein expression over 5 g/L subsequently. However, the incredible natural expression system possesses more potential beyond the past comprehension. During years’ trials and accumulations of experiences, the creative scientists at Creative Biolabs have released the hidden potential of B. subtilis greatly.

Our magic service possesses a series of superior services to achieve the most optimal yield and bioactivity based on every specific target protein. The scientists of Creative Biolabs stood on the basic induction mechanism originally proposed by several creative researchers and subsequently redeveloped the sugar-inducible B. subtilis expression system with multiple creative optimizations almost in every single step. Thus, the MagicTM Sugar-inducible Bacillus subtilis Expression Service offers researcher the first-rate B. subtilis expression services in the world. If you have any question, don’t hesitate to contact us. The genuine help on researcher’s valuable project is our best pleasure.

Reference

1. Sun, Tianqi, and Josef Altenbuchner. "Characterization of a mannose utilization system in Bacillus subtilis." Journal of bacteriology 192.8 (2010): 2128-2139.

For research use only. Not intended for any clinical use.