Recombinant Rabbit Anti-MSN Antibody (clone R08-3G5)
CAT#: VS3-FY965
This product is a recombinant rabbit antibody that recognizes MSN. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R08-3G5 is specific for human, mouse, rat MSN. The antigen is synthetic peptide of human moesin.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10898/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47336/if_selected.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Low Intensity: Moderate Quantity: <25% Alveolar cells type I I Staining: Medium Intensity: Moderate Quantity: 75%-25% Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Macrophages Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_1_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147065-MSN
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IHC-P
Figure 1. Recombinant Rabbit Anti-MSN Antibody (clone R08-3G5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Moesin antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human Moesin.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MSN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-3G5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 68 kDa; Observed MW: 68 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Moesin; Membrane-Organizing Extension Spike Protein; Epididymis Luminal Protein 70; HEL70; IMD50
- Gene ID
- 4478
- UniProt ID
- P26038
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane
- Post Translation Modifications
- Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).
Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.
S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex.
- Protein Refseq
- NP_002435.1
- Function
- Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton (PubMed:10212266).
Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement (PubMed:10212266).
These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction (PubMed:12387735, PubMed:15039356).
The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs (PubMed:9298994, PubMed:9616160).
Modulates phagolysosomal biogenesis in macrophages (By similarity).
Participates also in immunologic synapse formation (PubMed:27405666).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MSN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2005z | Mouse Anti-MSN Recombinant Antibody (clone 9C10) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MRO-1015-CN | Recombinant Rabbit Anti-MSN Monoclonal Antibody (CBACN-379) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS-0923-FY117 | Recombinant Mouse Anti-MSN Antibody (VS-0923-FY117) | ELISA, WB | Mouse IgG1 |
| VS3-FY2809 | Rabbit Anti-MSN Recombinant Antibody (VS3-FY2809) | WB, IHC, IP | Rabbit IgG |
| VS13-YC744 | Rabbit Anti-MSN Recombinant Antibody (VS13-YC744) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2304 | Hi-Affi™ Recombinant Rabbit Anti-MSN Monoclonal Antibody (DS2304AB) | IHC-FoFr, WB, IP, ICC, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC249 | AbPlus™ Anti-MSN Magnetic Beads (VS-0724-YC249) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY326 | Mouse Anti-NHP MSN Recombinant Antibody (clone 2C12) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1380 | Anti-MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4538 | Anti-Mouse MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4539 | Anti-Rat MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4537 | Anti-Human MSN Immunohistochemistry Kit | IHC |
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