Recombinant Mouse Anti-MYC Antibody (VS4-CJ47) (CAT#: VS4-CJ47)

• Immunogen
• Peptide sequence derived from C-terminal (410aa-432aa) of human c-Myc conjugated with KLH.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human MYC

• Species Reactivity
• Human

• Applications
• ELISA, WB

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 150mM NaCl, PBS, pH 7.4.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000).

• Alternative Names
• MYC proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

• Gene ID
• 4609

• UniProt ID
• P01106

• Long Name
• MYC proto-oncogene, bHLH transcription factor

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated by PRKDC (PubMed:1597196).
  Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity).
  Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526).
  Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58 (PubMed:22307329).
  Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367).
  Dephosphorylation at Ser-62 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25803737, PubMed:25438055).
  Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:25775507).
  In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation (PubMed:17873522, PubMed:17558397).
  In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus (PubMed:17558397, PubMed:25775507).
  Also polyubiquitinated by the DCX(TRPC4AP) complex (PubMed:20551172, PubMed:29779948).
  Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

• Protein Refseq
• NP_002458.2

• Function
• Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029).
  Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029).
  Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029).
  Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity).
  Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).
  Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS