Recombinant Mouse Anti-TOP1 Antibody (clone 3D4B1) (CAT#: VS3-FY1470)

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Clone
• 3D4B1

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry.

• Alternative Names
• DNA Topoisomerase I; Topoisomerase (DNA) I; EC 5.99.1.2; Type I DNA Topoisomerase; DNA Topoisomerase 1; TOPI

• Gene ID
• 7150

• UniProt ID
• P11387

• Sequence Similarities
• Belongs to the type IB topoisomerase family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.

• Protein Refseq
• NP_003277.1

• Function
• Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

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