Recombinant Mouse Anti-TOP1 Antibody (clone 3D4B1)
CAT#: VS3-FY1470
This product is a recombinant mouse antibody that recognizes TOP1. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry. The clone 3D4B1 is specific for TOP1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/154_H7_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows general nuclear positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_3_2_selected.jpg
Subcellular Location
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9058/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/198_H7_1_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/152_H7_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_8_5.jpg
Normal Tissue
Figure 9 Urinary bladder
Urothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_5_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19039/43952_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198900-TOP1
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- 3D4B1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- DNA Topoisomerase I; Topoisomerase (DNA) I; EC 5.99.1.2; Type I DNA Topoisomerase; DNA Topoisomerase 1; TOPI
- Gene ID
- 7150
- UniProt ID
- P11387
- Sequence Similarities
- Belongs to the type IB topoisomerase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
- Protein Refseq
- NP_003277.1
- Function
- Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TOP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1216z | Mouse Anti-TOP1 Recombinant Antibody (clone 38C7) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1811MZ | Recombinant Mouse Anti-Human TOP1 Antibody (clone 23B11) | IHC-P, WB | Mouse antibody |
| VS3-QX1142 | Mouse Anti-TOP1 Recombinant Antibody (clone 1291CT875.142.166) | WB, FC | Mouse IgG1, κ |
| VS3-CJ591 | Mouse Anti-TOP1 Recombinant Antibody (VS3-CJ591) | WB, FC | Mouse IgG1, κ |
| VS3-CJ592 | Rabbit Anti-TOP1 Recombinant Antibody (VS3-CJ592) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3638 | Rabbit Anti-TOP1 Recombinant Antibody (clone DS3638AB) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0004JF-F(E) | Chicken Anti-TOP1 Recombinant Antibody (clone 5C2); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0004JF-S(P) | Chicken Anti-TOP1 Recombinant Antibody (clone 5C2); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7424 | Anti-TOP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7425 | Anti-Mouse TOP1 Immunohistochemistry Kit | IHC |
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