Recombinant Human Anti-CD20 Antibody (Ofatumumab) (CAT#: PABX-028)

Anti-CD20 antibody is a Human antibody of IgG1 κ class that binds to an CD20.

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Apop

Figure 1 Ofatumumab and rituximab exhibit modest direct antitumor activity against MCL cell lines.

MCL cell lines were utilized in alamarBlue reduction assays to investigate the direct antilymphoma activity of each antibody. Each antibody exhibited a time-dependent decrease in MCL cell viability following exposure to 10 μg/mL of each respective antibody in the presence of a cross-linking IgG antibody. With the exception of the Mino cell line at the 72-hour time point, all cell lines demonstrated similar degrees of direct antitumor activity with ofatumumab and rituximab at the dose tested (*, P < 0.05).

Barth, M. J., Mavis, C., Czuczman, M. S., & Hernandez-Ilizaliturri, F. J. (2015). Ofatumumab exhibits enhanced in vitro and in vivo activity compared to rituximab in preclinical models of mantle cell lymphoma. Clinical Cancer Research, 21(19), 4391-4397.

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Figure 2 Ofatumumab induces significantly higher levels of CDC-associated cell lysis than rituximab.

MCL tumor cell lines were tested for CDC cell lysis using a 51Cr release assay with human serum from normal healthy donors as a source of complement. In all MCL cell lines tested, with the exception of Granta, ofatumumab was more effective than rituximab at inducing CDC lysis at a dose of 10 μg/mL. (*, P < 0.05).

Barth, M. J., Mavis, C., Czuczman, M. S., & Hernandez-Ilizaliturri, F. J. (2015). Ofatumumab exhibits enhanced in vitro and in vivo activity compared to rituximab in preclinical models of mantle cell lymphoma. Clinical Cancer Research, 21(19), 4391-4397.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human CD20
  • Species Reactivity
  • Human
  • Clone
  • Ofatumumab
  • Applications
  • WB, ELISA, IF, FuncS, Apop, BA

Applications

  • Application Notes
  • The MS4A1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Functional Assay, Apoptosis, Bioassay.

Target

  • Alternative Names
  • MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "MS4A1"

See other products for "Clone Ofatumumab"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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