Recombinant Human Anti-CD20 Antibody (Ofatumumab) (CAT#: PABX-028)

Anti-CD20 antibody is a Human antibody of IgG1 κ class that binds to an CD20.

Host: Human

Isotype: IgG

Applications: WB, ELISA, IF, FuncS, Apop, BA


Specific Inquiry
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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
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  • COA
Apop

Figure 1 Ofatumumab and rituximab exhibit modest direct antitumor activity against MCL cell lines.

MCL cell lines were utilized in alamarBlue reduction assays to investigate the direct antilymphoma activity of each antibody. Each antibody exhibited a time-dependent decrease in MCL cell viability following exposure to 10 μg/mL of each respective antibody in the presence of a cross-linking IgG antibody. With the exception of the Mino cell line at the 72-hour time point, all cell lines demonstrated similar degrees of direct antitumor activity with ofatumumab and rituximab at the dose tested (*, P < 0.05).

Barth, M. J., Mavis, C., Czuczman, M. S., & Hernandez-Ilizaliturri, F. J. (2015). Ofatumumab exhibits enhanced in vitro and in vivo activity compared to rituximab in preclinical models of mantle cell lymphoma. Clinical Cancer Research, 21(19), 4391-4397.

BA

Figure 2 Ofatumumab induces significantly higher levels of CDC-associated cell lysis than rituximab.

MCL tumor cell lines were tested for CDC cell lysis using a 51Cr release assay with human serum from normal healthy donors as a source of complement. In all MCL cell lines tested, with the exception of Granta, ofatumumab was more effective than rituximab at inducing CDC lysis at a dose of 10 μg/mL. (*, P < 0.05).

Barth, M. J., Mavis, C., Czuczman, M. S., & Hernandez-Ilizaliturri, F. J. (2015). Ofatumumab exhibits enhanced in vitro and in vivo activity compared to rituximab in preclinical models of mantle cell lymphoma. Clinical Cancer Research, 21(19), 4391-4397.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human CD20
  • Species Reactivity
  • Human
  • Clone
  • Ofatumumab
  • Applications
  • WB, ELISA, IF, FuncS, Apop, BA

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The MS4A1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Functional Assay, Apoptosis, Bioassay.

Target

  • Alternative Names
  • MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-913-sdAb Recombinant Anti-human MS4A1 VHH Single Domain Antibody IP, FC, ChiP, Neut Llama VHH
HPAB-M0704-YC Camelid Anti-MS4A1 Recombinant Antibody (clone 2HCD25) ELISA, FuncS Camelid VHH
HPAB-M0705-YC Camelid Anti-MS4A1 Recombinant Antibody (clone 2HCD16) ELISA Camelid VHH
HPAB-M0706-YC Camelid Anti-MS4A1 Recombinant Antibody (clone R3CD7) ELISA Camelid VHH
HPAB-M0707-YC Camelid Anti-MS4A1 Recombinant Antibody (clone 2HCD78) ELISA Camelid VHH

Chimeric Antibody

CAT Product Name Application Type
TAB-1665CL Human Anti-MS4A1 Recombinant Antibody (TAB-1665CL) CDC Chimeric (mouse/human) IgG
TAB-1633CL-S(P) Anti-Human MS4A1 Recombinant Antibody scFv Fragment (ch1F5) FC Chimeric antibody (mouse/human)
TAB-1634CL-S(P) Anti-Human MS4A1 Recombinant Antibody scFv Fragment (ch1F5-DXL) FC, Apop, CDC, ADCC, Inhib Chimeric antibody (mouse/human)
TAB-1656CL-S(P) Anti-Human MS4A1 Recombinant Antibody scFv Fragment (ch2B8) FC, CMCD, ADCC, Activ, Inhib, Depletion Chimeric antibody (mouse/human)
PABX-026 Recombinant Human Anti-CD20 Antibody (C2H7) FuncS IgG

Humanized Antibody

CAT Product Name Application Type
TAB-1677CL Anti-Human MS4A1 Recombinant Antibody (IBI-301) PK Humanized antibody
TAB-1694CL Human Anti-MS4A1 Recombinant Antibody (TAB-1694CL) CDC Humanized IgG
TAB-1695CL Human Anti-MS4A1 Recombinant Antibody (TAB-1695CL) Cyt, ELISA, FuncS Humanized IgG
TAB-1696CL Human Anti-MS4A1 Recombinant Antibody (TAB-1696CL) Cyt, ELISA, FuncS Humanized IgG
TAB-1697CL Human Anti-MS4A1 Recombinant Antibody (TAB-1697CL) Cyt, ELISA, FuncS Humanized IgG

Human Antibody

CAT Product Name Application Type
TAB-1703CL Human Anti-MS4A1 Recombinant Antibody (TAB-1703CL) FC, CDC, IP Human IgG1, κ
TAB-1648CL-F(E) Anti-MS4A1 Recombinant Antibody Fab Fragment (MEDI-552) Inhib
TAB-1654CL-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1654CL-F(E)) FC, Apop Human Fab
TAB-1655CL-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1655CL-F(E)) ELISA Human Fab
TAB-1676CL-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1676CL-F(E)) ELISA Human Fab

Mouse Antibody

CAT Product Name Application Type
TAB-1659CL-F(E) Anti-Human MS4A1 Recombinant Antibody Fab Fragment (1K0924) Apop, FuncS
TAB-1660CL-F(E) Anti-Human MS4A1 Recombinant Antibody Fab Fragment (1K1712) Apop, FuncS
PABX-026-S (P) Recombinant Mouse Anti-CD20 Antibody scFv Fragment (C2H7) WB, ELISA, FuncS scFv
PABX-028-S (P) Recombinant Human Anti-CD20 Antibody scFv Fragment (Ofatumumab) WB, ELISA, IF, FuncS scFv
TAB-007ML Anti-Canine MS4A1 Recombinant Antibody (Blontuvetmab) ELISA, IHC, FC, IP, IF, FuncS IgG2, κ, λ

Fc Glycosylation

CAT Product Name Application Type
Gly-059LC Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)
Gly-060LC Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) ELISA Humanized antibody
Gly-062LC Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)
Gly-063LC Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Chimeric antibody (mouse/human)
Gly-067LC Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation) ELISA, FC Chimeric antibody (mouse/human)

Low- or Non-fucosylated Oligosaccharide

CAT Product Name Application Type
Gly-134LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Humanized antibody
Gly-135LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Humanized antibody
Gly-136LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Humanized antibody
Gly-137LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Humanized antibody
Gly-138LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) ELISA Humanized antibody

Hyper-galactosylated

CAT Product Name Application Type
Gly-142LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Hyper-galactosylated) ELISA Human antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-155LC-1 Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Humanized antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-171LC Recombinant Anti-Human MS4A1 Antibody (Non-glycosylated) ELISA Chimeric antibody (mouse/human)

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC066 A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer FCM
MHC-LC067 A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer FCM
MHC-LC068 A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer FCM
MHC-LC4347 PE-B*07:02/Human MS4A1 (RPKSNIVLL) MHC Tetramer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1751CQ Mouse Anti-MS4A1 Recombinant Antibody (clone CBLS-211) Neut, FuncS Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2295 Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone DS2295AB) IHC-Fr, IHC-P Rabbit IgG
MOR-4024 Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone SI88DS) FC Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
HPAB-0018-FY Human Anti-MS4A1 Recombinant Antibody (HPAB-0018-FY) FC Humanized IgG1
HPAB-0021-FY Human Anti-MS4A1 Recombinant Antibody (clone hu3D9) FC Humanized IgG
HPAB-0683-CN Human Anti-MS4A1 Recombinant Antibody (clone Rmut8) ELISA, FC Chimeric (mouse/human) IgG
HPAB-0684-CN Human Anti-MS4A1 Recombinant Antibody (clone Rmut5) ELISA, FC Chimeric (mouse/human) IgG
MRO-0288-CN Rabbit Anti-MS4A1 Recombinant Antibody (clone CBACN-109) WB, IHC, IP Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0683-CN-F(E) Human Anti-MS4A1 Recombinant Antibody (clone Rmut8); Fab Fragment ELISA, FC Chimeric (mouse/human) Fab
HPAB-0684-CN-F(E) Human Anti-MS4A1 Recombinant Antibody (clone Rmut5); Fab Fragment ELISA, FC Chimeric (mouse/human) Fab
HPAB-0742-CN-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-0742-CN-F(E)) ELISA, FC Human Fab
HPAB-0743-CN-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-0743-CN-F(E)) ELISA, FC Human Fab
HPAB-0247-FY-F(E) Human Anti-MS4A1 Recombinant Antibody (clone EM201); Fab Fragment Inhib, ELISA Humanized Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-772 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocrelizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-016 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Rituximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-028 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ofatumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-207 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ublituximab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-771 Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocaratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1336-FY-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1336-FY-F(E)) FC, ELISA Human Fab
HPAB-1337-FY-F(E) Mouse Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1337-FY-F(E)) ELISA Mouse Fab
HPAB-1775-FY-F(E) Mouse Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1775-FY-F(E)) ELISA, Activ Mouse Fab
HPAB-1885-FY-F(E) Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1885-FY-F(E)) ELISA, FC Human Fab
HPAB-1954-FY-S(P) Human Anti-MS4A1 Recombinant Antibody (clone BD31-7); scFv Fragment Cyt Humanized scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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