anti-CD22 immunotoxin RFB4 (scFv)-DT (CAT#: AGTO-L048D)

This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Diphtheriatoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells. Once bind, the toxin enters cells by receptor-mediated endocytosis, then Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme; -II binding and elongation factor into the eukaryotic cell; the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs; thereby preventing protein synthesis and causes cell death. It was designed for treatment of leukemia.

Host: Mouse

Applications: This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.


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Normal Tissue

RNA Expression


Specifications

  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • RFB4
  • Molecule Class
  • scFv-DT
  • Toxin Description
  • Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
  • Applications
  • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
  • Related Disease
  • Leukemia

Target

  • Alternative Names
  • CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB389

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Humanized Antibody

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TAB-176 Anti-Human CD22 Recombinant Antibody (Epratuzumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1
TAB-899 Humanized Anti-CD22 Recombinant Antibody (clone Pinatuzumab) FC, IP, ELISA, Neut, FuncS, IF, ICC Humanized (from mouse) IgG1, κ
TAB-1725CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) Block, Cyt, ELISA, FuncS Humanized Fab
TAB-1729CL-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) IF, FC Humanized Fab
TAB-002LC Anti-Human CD22 Recombinant Antibody (VM1000) FC, ELISA, SPR, FuncS Humanized antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G077E Anti-CD22 immunotoxin RFB4 (IgG)-PE Cytotoxicity assay, Function study
AGTO-L048E anti-CD22 immunotoxin RFB4 (scFv)-PE Cytotoxicity assay, Functional assay
AGTO-L048G anti-CD22 immunotoxin RFB4 (scFv)-Gel Cytotoxicity assay, Functional assay
AGTO-L048R anti-CD22 immunotoxin RFB4 (scFv)-RTA Cytotoxicity assay, Functional assay
AGTO-L048S anti-CD22 immunotoxin RFB4 (scFv)-Sap Cytotoxicity assay, Functional assay

Chimeric Antibody

CAT Product Name Application Type
TAB-1724CL Human Anti-CD22 Recombinant Antibody (TAB-1724CL) Inhib Chimeric (mouse/human) IgG1, κ
TAB-1724CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) Inhib Mouse scFv
TAB-001LC Human Anti-CD22 Recombinant Antibody (TAB-001LC) ELISA Chimeric antibody (mouse/human)
TAB-001LC-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) ELISA Mouse scFv
TAB-001LC-F(E) Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) ELISA Chimeric antibody (mouse/human)

Mouse Antibody

CAT Product Name Application Type
TAB-1714CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P)) ELISA, WB Mouse scFv
TAB-1723CL-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1723CL-S(P)) Inhib Mouse scFv
TAB-1728CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (RFB4) IF, FC
TAB-1714CL-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1714CL-F(E)) ELISA, WB Mouse Fab
TAB-1723CL-F(E) Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1723CL-F(E)) Inhib Mouse Fab

Human Antibody

CAT Product Name Application Type
TAB-1721CL-S(P) Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) ELISA, FC, FuncS Human antibody
TAB-0196CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2) ELISA, In vivo
TAB-0197CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2-Cys) ELISA, In vivo
TAB-1721CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.1) ELISA, FC, FuncS Human antibody
TAB-1722CL-F(E) Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.2) ELISA, FC, FuncS Human antibody

Fab Glycosylation

CAT Product Name Application Type
Gly-001LC Recombinant Anti-Human CD22 Antibody (Fab glycosylation) ELISA, FC Mouse antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-111LC Recombinant Anti-Human CD22 Antibody (Fc glycosylation) ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-315CQ Rat Anti-CD22 Recombinant Antibody (clone 2D6) Block Rat IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0545 Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) IP, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-176 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-198 Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

Recombinant Antibody

CAT Product Name Application Type
FN-070CQ Mouse Anti-CD22 Recombinant Antibody (clone IS7) FC Mouse IgG1
FN-088CQ Mouse Anti-CD22 Recombinant Antibody (clone Cy34.1) Depletion, FC, IP Mouse IgG1
HPAB-0370-FY Human Anti-CD22 Recombinant Antibody (HPAB-0370-FY) FC, FuncS, ELISA Humanized IgG
HPAB-0371-FY Human Anti-CD22 Recombinant Antibody (HPAB-0371-FY) Inhib Humanized IgG
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scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1899LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1899LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1900LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1900LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1901LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1901LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1902LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1902LY-S(P)) ELISA, Inhib Mouse scFv
HPAB-1903LY-S(P) Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1903LY-S(P)) ELISA, Inhib Mouse scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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