anti-CD22 immunotoxin RFB4 (scFv)-DT (CAT#: AGTO-L048D)
This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Diphtheriatoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells. Once bind, the toxin enters cells by receptor-mediated endocytosis, then Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme; -II binding and elongation factor into the eukaryotic cell; the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs; thereby preventing protein synthesis and causes cell death. It was designed for treatment of leukemia.
Host: Mouse
Applications: This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
Specific Inquiry
Normal Tissue
Figure 1 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
Figure 2 Spleen
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- RFB4
- Molecule Class
- scFv-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leukemia
Target
- Alternative Names
- CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB389
- Gene ID
- 12483
- UniProt ID
- P35329
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| MOB-1248z-F(E) | Recombinant Anti-Human CD22 Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| HPAB-0194-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) | ELISA | Mouse Fab |
| HPAB-0195-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0196-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) | ELISA | Mouse Fab |
| HPAB-0197-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) | ELISA | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
| TAB-176 | Anti-Human CD22 Recombinant Antibody (Epratuzumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 |
| TAB-899 | Humanized Anti-CD22 Recombinant Antibody (clone Pinatuzumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | Humanized (from mouse) IgG1, κ |
| TAB-1725CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) | Block, Cyt, ELISA, FuncS | Humanized Fab |
| TAB-1729CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) | IF, FC | Humanized Fab |
| TAB-002LC | Anti-Human CD22 Recombinant Antibody (VM1000) | FC, ELISA, SPR, FuncS | Humanized antibody |
Immunotoxin
| CAT | Product Name | Application | Type |
| AGTO-G077E | Anti-CD22 immunotoxin RFB4 (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-L048E | anti-CD22 immunotoxin RFB4 (scFv)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L048G | anti-CD22 immunotoxin RFB4 (scFv)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L048R | anti-CD22 immunotoxin RFB4 (scFv)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L048S | anti-CD22 immunotoxin RFB4 (scFv)-Sap | Cytotoxicity assay, Functional assay |
Chimeric Antibody
| CAT | Product Name | Application | Type |
| TAB-1724CL | Human Anti-CD22 Recombinant Antibody (TAB-1724CL) | Inhib | Chimeric (mouse/human) IgG1, κ |
| TAB-1724CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) | Inhib | Mouse scFv |
| TAB-001LC | Human Anti-CD22 Recombinant Antibody (TAB-001LC) | ELISA | Chimeric antibody (mouse/human) |
| TAB-001LC-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) | ELISA | Mouse scFv |
| TAB-001LC-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) | ELISA | Chimeric antibody (mouse/human) |
Mouse Antibody
| CAT | Product Name | Application | Type |
| TAB-1714CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P)) | ELISA, WB | Mouse scFv |
| TAB-1723CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1723CL-S(P)) | Inhib | Mouse scFv |
| TAB-1728CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (RFB4) | IF, FC | |
| TAB-1714CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1714CL-F(E)) | ELISA, WB | Mouse Fab |
| TAB-1723CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1723CL-F(E)) | Inhib | Mouse Fab |
Human Antibody
| CAT | Product Name | Application | Type |
| TAB-1721CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
| TAB-0196CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2) | ELISA, In vivo | |
| TAB-0197CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (hLL2-Cys) | ELISA, In vivo | |
| TAB-1721CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
| TAB-1722CL-F(E) | Anti-Human CD22 Recombinant Antibody Fab Fragment (CD22.2) | ELISA, FC, FuncS | Human antibody |
Fab Glycosylation
| CAT | Product Name | Application | Type |
| Gly-001LC | Recombinant Anti-Human CD22 Antibody (Fab glycosylation) | ELISA, FC | Mouse antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
| Gly-111LC | Recombinant Anti-Human CD22 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
Blocking Antibody
| CAT | Product Name | Application | Type |
| NEUT-315CQ | Rat Anti-CD22 Recombinant Antibody (clone 2D6) | Block | Rat IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-0545 | Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) | IP, WB | Rabbit IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
| AFC-TAB-176 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-198 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
| FN-070CQ | Mouse Anti-CD22 Recombinant Antibody (clone IS7) | FC | Mouse IgG1 |
| FN-088CQ | Mouse Anti-CD22 Recombinant Antibody (clone Cy34.1) | Depletion, FC, IP | Mouse IgG1 |
| HPAB-0370-FY | Human Anti-CD22 Recombinant Antibody (HPAB-0370-FY) | FC, FuncS, ELISA | Humanized IgG |
| HPAB-0371-FY | Human Anti-CD22 Recombinant Antibody (HPAB-0371-FY) | Inhib | Humanized IgG |
| ZG-0989F | Mouse Anti-CD22 Recombinant Antibody (clone 3H4) | FC, IHC-Fr, ICC, IHC-Fr | Mouse IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-1899LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1899LY-S(P)) | ELISA, Inhib | Mouse scFv |
| HPAB-1900LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1900LY-S(P)) | ELISA, Inhib | Mouse scFv |
| HPAB-1901LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1901LY-S(P)) | ELISA, Inhib | Mouse scFv |
| HPAB-1902LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1902LY-S(P)) | ELISA, Inhib | Mouse scFv |
| HPAB-1903LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1903LY-S(P)) | ELISA, Inhib | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
AgonistAgonist
BABioassay
InhibInhibition
PGProteogenomics
StimStimulation
WBWestern Blot
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