anti-CD22 immunotoxin RFB4 (scFv)-DT
CAT#: AGTO-L048D
This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Diphtheriatoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells. Once bind, the toxin enters cells by receptor-mediated endocytosis, then Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme; -II binding and elongation factor into the eukaryotic cell; the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs; thereby preventing protein synthesis and causes cell death. It was designed for treatment of leukemia.



Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- RFB4
- Molecule Class
- scFv-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leukemia
Target
- Alternative Names
- CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB389
- Gene ID
- 12483
- UniProt ID
- P35329
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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See other products for "Clone RFB4"
- CAT
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CAT | Product Name | Application | Type |
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AGTO-G077E | Anti-CD22 immunotoxin RFB4 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-L048E | anti-CD22 immunotoxin RFB4 (scFv)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L048G | anti-CD22 immunotoxin RFB4 (scFv)-Gel | Cytotoxicity assay, Functional assay | |
AGTO-L048R | anti-CD22 immunotoxin RFB4 (scFv)-RTA | Cytotoxicity assay, Functional assay | |
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TAB-0196CL | Anti-Human CD22 Recombinant Antibody (hLL2) | Inhib, Cyt, FC, WB | |
TAB-1721CL-S(P) | Human Anti-CD22 Recombinant Antibody scFv Fragment (TAB-1721CL-S(P)) | ELISA, FC, FuncS | Human antibody |
TAB-1722CL-S(P) | Human Anti-CD22 Recombinant Antibody scFv Fragment (TAB-1722CL-S(P)) | ELISA, FC, FuncS | Human antibody |
TAB-1721CL-F(E) | Human Anti-CD22 Recombinant Antibody Fab Fragment (TAB-1721CL-F(E)) | ELISA, FC, FuncS | Human antibody |
TAB-1722CL-F(E) | Human Anti-CD22 Recombinant Antibody Fab Fragment (TAB-1722CL-F(E)) | ELISA, FC, FuncS | Human antibody |
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TAB-1724CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) | Inhib | Mouse scFv |
TAB-1724CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1724CL-F(E)) | Inhib | Chimeric (mouse/human) Fab |
TAB-001LC | Human Anti-CD22 Recombinant Antibody (TAB-001LC) | ELISA | Chimeric antibody (mouse/human) |
TAB-001LC-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
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TAB-1714CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P)) | ELISA, WB | Mouse scFv |
TAB-1723CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1723CL-S(P)) | Inhib | Mouse scFv |
TAB-1728CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (RFB4) | IF, FC | |
TAB-1714CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1714CL-F(E)) | ELISA, WB | Mouse Fab |
TAB-1723CL-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1723CL-F(E)) | Inhib | Mouse Fab |
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TAB-1725CL-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1725CL-S(P)) | Block, Cyt, ELISA, FuncS | Humanized scFv |
TAB-1729CL-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1729CL-S(P)) | IF, FC | Humanized scFv |
TAB-1725CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) | Block, Cyt, ELISA, FuncS | Humanized Fab |
TAB-1729CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) | IF, FC | Humanized Fab |
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CAT | Product Name | Application | Type |
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CAT | Product Name | Application | Type |
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HPAB-N0324-YC-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-N0324-YC-F(E)) | FuncS | Mouse Fab |
HPAB-N0325-YC-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-N0325-YC-F(E)) | FuncS | Mouse Fab |
HPAB-0034-YJ-F(E) | Mouse Anti-CD22 Recombinant Antibody (clone HB22-23); Fab Fragment | Block | Mouse Fab |
HPAB-0370-FY-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0370-FY-F(E)) | ELISA | Humanized Fab |
HPAB-0371-FY-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0371-FY-F(E)) | Inhib | Humanized Fab |
CAT | Product Name | Application | Type |
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HPAB-0249-FY-S(P) | Recombinant Human Anti-CD22 Antibody scFv Fragment | F | Human scFv |
HPAB-1899LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1899LY-S(P)) | ELISA, Inhib | Mouse scFv |
HPAB-1900LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1900LY-S(P)) | ELISA, Inhib | Mouse scFv |
HPAB-1901LY-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-1901LY-S(P)) | ELISA, Inhib | Mouse scFv |
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AFC-TAB-176 | Afuco™ Anti-CD22 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-176) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-198 | Afuco™ Anti-CD22 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-198) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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HPAB-1899LY | Mouse Anti-CD22 Recombinant Antibody (HPAB-1899LY) | ELISA, Inhib | Mouse IgG |
HPAB-1900LY | Mouse Anti-CD22 Recombinant Antibody (HPAB-1900LY) | ELISA, Inhib | Mouse IgG |
HPAB-1901LY | Mouse Anti-CD22 Recombinant Antibody (HPAB-1901LY) | ELISA, Inhib | Mouse IgG |
HPAB-1902LY | Mouse Anti-CD22 Recombinant Antibody (HPAB-1902LY) | ELISA, Inhib | Mouse IgG |
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