anti-CD22 immunotoxin RFB4 (scFv)-RTA (CAT#: AGTO-L048R)
This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to ricin A chain. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of leukemia.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Normal Tissue
RNA Expression
Figure 1 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
Figure 2 Spleen
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- RFB4
- Molecule Class
- scFv-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leukemia
Target
- Alternative Names
- CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA
- Gene ID
- 12483
- UniProt ID
- P35329
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone RFB4"
See other products for "CD22"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
Mouse Antibody Products
Immunotoxin Products
Chimeric Antibody Products
Humanized Antibody Products
Human Antibody Products
Fab Glycosylation Antibody Products
Fc Glycosylation Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
ADCC Enhanced Antibody Products
Antibody Magnetic Beads
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1248z-F(E) | Recombinant Anti-Human CD22 Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| HPAB-N0324-YC-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-N0324-YC-F(E)) | FuncS | Mouse Fab |
| HPAB-0034-YJ-F(E) | Mouse Anti-CD22 Recombinant Antibody (clone HB22-23); Fab Fragment | Block | Mouse Fab |
| HPAB-0709-FY-S(P) | Mouse Anti-CD22 Recombinant Antibody (clone AB008); Fab Fragment | ELISA | Mouse Fab |
| HPAB-2052-FY-F(E) | Human Anti-CD22 Recombinant Antibody (clone B28); Fab Fragment | FC | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for AGTO-L048R. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, WB, BLI, SPR
Application: Block, Cyt, FuncS, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, BL
Application: ELISA, Inhib, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, FC, Inhib
Application: ELISA, FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
