anti-CD22 immunotoxin RFB4 (scFv)-PE (CAT#: AGTO-L048E)

This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.

Specific Inquiry
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  • Gene Expression
  • Datasheet
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Normal Tissue
RNA Expression

Specifications

  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • RFB4
  • Molecule Class
  • scFv-PE
  • Toxin Description
  • Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
  • Applications
  • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
  • Related Disease
  • Leukemia

Target

  • Alternative Names
  • CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Customer Reviews and Q&As

Customer Review Q&As

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Effective Targeting Tool
We used Anti-CD22 Immunotoxin RFB4 in our B-cell lymphomas and observed substantial cytotoxicity. The specificity for CD22 was remarkable, and off-target effects were minimal, leading to clearer results in our xenograft models.

High Potency
The potency of the immunotoxin was evident in our in vitro experiments. It effectively induced apoptosis in CD22-expressing cells, proving to be a valuable tool for our B-cell malignancy studies.

Minimal Off-Target Effects
Off-target effects were a concern, but this immunotoxin showed minimal adverse effects on non-CD22 cells. It reflects well on its design and utility for specific targeting.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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