anti-CD22 immunotoxin RFB4 (scFv)-PE
CAT#: AGTO-L048E
This immunotoxin was achieved by conjugating/fusing the Anti-CD22 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of leukemia.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
Normal Tissue
Figure 2 Spleen
Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
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Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- RFB4
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leukemia
Target
- Alternative Names
- CD22; CD22 antigen; Lyb8; Lyb-8; A530093D23; B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2; Leukemia; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35
- Gene ID
- 12483
- UniProt ID
- P35329
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Effective Targeting Tool
We used Anti-CD22 Immunotoxin RFB4 in our B-cell lymphomas and observed substantial cytotoxicity. The specificity for CD22 was remarkable, and off-target effects were minimal, leading to clearer results in our xenograft models.
High Potency
The potency of the immunotoxin was evident in our in vitro experiments. It effectively induced apoptosis in CD22-expressing cells, proving to be a valuable tool for our B-cell malignancy studies.
Minimal Off-Target Effects
Off-target effects were a concern, but this immunotoxin showed minimal adverse effects on non-CD22 cells. It reflects well on its design and utility for specific targeting.
Q&As
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How does the scFv fragment contribute to the efficacy of this immunotoxin?
A: The scFv (single-chain variable fragment) allows for strong and specific binding to the CD22 antigen, enhancing the targeted toxicity and improving the specificity of the immunotoxin against B-cell tumors.
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What type of experimental controls should be used with Anti-CD22 Immunotoxin RFB4 (scFv)-PE?
A: Negative controls like irrelevant scFv or non-binding immunotoxin should be used alongside untreated samples to validate the target-specific cytotoxic effects.
-
How does the immunotoxin's specificity impact off-target effects?
A: The high specificity due to the scFv reduces off-target effects, minimizing damage to non-B cells and thus lowering potential systemic toxicity.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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