Anti-CD28 Antibody-Cytokine Fusion Protein, TNF-IgG (15E8) (CAT#: ACFP-SH131)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD28 IgG to TNF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD28 as well as the biological activity of TNF. TNF-FuP provided a weak stimulus for lymphocyte proliferation, but it had no effect on LAK cells. However, it supported activation of Mfs and, to a minor extent, of CTLs. This immunocytokine was designed for treating tumor.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Figure 2 Tonsil
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
Specifications
- Target
- CD28
- Molecule Class
- TNF-IgG2a
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- 15E8
- Related Disease
- Tumor
- Cytokine 1
- TNF
- Cytokines 1 Description
- Tumor necrosis factor (TNF), formerly known as TNFα or TNF alpha, is the best-known member of this class. TNF is a monocyte-derived cytotoxin that has been implicated in tumor regression, septic shock, and cachexia. The protein is synthesized as a prohormone with an unusually long and atypical signal sequence, which is absent from the mature secreted cytokine. A short hydrophobic stretch of amino acids serves to anchor the prohormone in lipid bilayers. Both the mature protein and a partially processed form of the hormone can be secreted after cleavage of the propeptide.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
- Gene ID
- 940
- UniProt ID
- P31041
Related Resources
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
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See other products for "Clone 15E8"
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0013-CN-S(P) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); scFv Fragment | FC | Mouse scFv |
| HPAB-0132CQ-S(P) | Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment | ELISA, IHC, Block | Human scFv |
| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody (clone 2E12); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-064LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (TGN1412) | FC | Humanized antibody |
| TAB-065LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-065LC-S(P)) | ELISA | Human scFv |
| TAB-066LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-066LC-S(P)) | ELISA | Human scFv |
| TAB-069LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (HuTN228) | FC, ELISA | Humanized antibody |
| TAB-064LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (TGN1412) | FC | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031 | Recombinant Mouse Anti-CD28 Antibody (5.11A1) | WB, ELISA, FuncS, Activ | IgG |
| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (5.11A1) | WB, ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (5.11A1) | WB, ELISA, FuncS | scFv |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
| NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
| NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
| NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
| MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 1) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (re-CD28 clone 2) | ELISA | Humanized VH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0132CQ | Human Anti-CD28 Recombinant Antibody (clone FR255734) | ELISA, IHC, Block, FuncS | Human IgG2M3, κ |
| FN-227CQ | Mouse Anti-CD28 Recombinant Antibody (clone JJ319) | FC, FuncS | Mouse IgG1 |
| HPAB-0078-YJ | Mouse Anti-Cd28 Recombinant Antibody (clone JJ-316) | Costim, Agonist, FC, IF, IHC, FuncS | Mouse IgG1, κ |
| HPAB-0079-YJ | Mouse Anti-Cd28 Recombinant Antibody (clone JJ-319) | IP, FC | Mouse IgG1, κ |
| HPAB-1489-FY | Human Anti-CD28 Recombinant Antibody (clone 2E12) | WB, ELISA | Human IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody (clone 2E12); Fab Fragment | WB, ELISA | Human Fab |
| HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody (Lumretuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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