Anti-CD4 Antibody-Cytokine Fusion Protein, TNF-IgG (OKT4) (CAT#: ACFP-SH123)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD4 IgG to TNF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD4 as well as the biological activity of TNF. TNF-FuP significantly accumulated in the tumor, which led to retardation of tumor growth. The fusion protein stimulated cytokine production and cytotoxic activity of monocytes and, to a low degree, of T cells. The therapeutic effect was improved by intratumoral application. Importantly, the efficacy of TNF-FuP depended on the induction of an immune response in vivo. This immunocytokine was designed for treating tumor.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11/if_selected.jpg
Figure 2 RNA cell line category: Group enriched (HEL, HL-60, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4
Figure 2 RNA cell line category: Group enriched (HEL, HL-60, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4
Specifications
- Target
- CD4
- Molecule Class
- TNF-IgG2b
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- OKT4
- Related Disease
- Tumor
- Cytokine 1
- TNF
- Cytokines 1 Description
- Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). While still controversial, studies of depression and IBD are currently being linked to TNF levels. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- CD4; CD4 Molecule; T-Cell Surface Antigen T4/Leu-3; T-Cell Surface Glycoprotein CD4; CD4 Antigen; CD4mut; CD4 Receptor; CD4 Antigen (P55)
- Gene ID
- 920
- UniProt ID
- B4DT49
Related Resources
Primary Immunodeficiency
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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