Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab)

Tested Data
Gene Expression
Datasheet
MSDS
COA

SDS-PAGE

Figure 1 Recombinant Human Anti-EGFR Antibody scFv Fragment (PABX-052-S(P)) in SDS-PAGE

The purity of PABX-052-S(P) was greater than 95% as determined by SDS-PAGE.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue

Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
scFv

Specificity
Tested positive against native Hepatitis C virus Hepatitis C virus

Species Reactivity
Human

Clone
Nimotuzumab

Applications
Can be useful in applications such as: Western blot; Enzyme-linked Immunosorbent Assay; Functional Study

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

Alternative Names
EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; mAb806; LICR 806; ABT-806; ch806

Gene ID
1956

UniProt ID
P00533

Related Resources

Product Notes

Downloads

See other products for "Clone Nimotuzumab"

See other products for "EGFR"

Recombinant Antibody

CAT	Product Name	Application	Type
MOB-1078z	Mouse Anti-EGFR Recombinant Antibody (clone 42C11)	WB, ELISA, FC, IF, IHC, FuncS	Mouse IgG1, κ
PABL-080	Human Anti-EGFR Recombinant Antibody (PABL-080)	ELISA, WB, FuncS	Human IgG
PABZ-039	Mouse Anti-EGFR Recombinant Antibody (clone mAb528)	FC	Mouse IgG
PABW-039	Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225))	FuncS	Chimeric (mouse/human) IgG
PABC-040	Human Anti-EGFR Recombinant Antibody (clone MR1)	Block	Human IgG

Single-domain Antibody

CAT	Product Name	Application	Type
NABG-056	Recombinant Anti-Mouse Egfr VHH Single Domain Antibody	ELISA, IHC, FC, FuncS	Llama VHH
PNBL-016	Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12)	WB, ELISA	Llama VHH
PNBL-017	Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8)	FuncS, ELISA, IF	Llama VHH
PNBL-018	Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1)	FuncS, SPR	Llama VHH
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122)	ELISA, FC, IP, FuncS	Llama VHH

Chimeric Antibody

CAT	Product Name	Application	Type
TAB-H35	Anti-Human EGFR Recombinant Antibody (Futuximab)	IF, WB, Inhib	IgG1 - kappa
TAB-0225CL-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0225CL-F(E))	Block, Inhib, FuncS, Apop, In vivo	Chimeric (Mouse/Human) Fab
TAB-272MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E))	FuncS	Chimeric (mouse/human) Fab
TAB-302MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E))	ELISA	Chimeric (mouse/human) Fab
TAB-308MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-308MZ-F(E))	ELISA, FC	Chimeric antibody (mouse/human)

Human Antibody

CAT	Product Name	Application	Type
TAB-020	Anti-Human EGFR Recombinant Antibody (Panitumumab)	ELISA, IP, FC, FuncS, Neut, IF, ICC	IgG2 - kappa
TAB-299MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-299MZ-F(E))	Internalization Assay	Human Fab
TAB-313MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11)	ELISA, FC	Human antibody
TAB-314MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-26-11)	ELISA, FC	Human antibody
PABX-053-F (E)	Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11)	WB, ELISA, FuncS	Fab

Humanized Antibody

CAT	Product Name	Application	Type
TAB-165	Anti-Human EGFR Recombinant Antibody (Matuzumab)	Neut, ELISA, IF, IP, FuncS, FC, ICC	IgG1
TAB-710	Anti-EGFR Recombinant Antibody (Nimotuzumab)	ELISA, IP, FC, FuncS, Neut, IF, IHC	IgG1 - kappa
TAB-274MZ	Human Anti-EGFR Recombinant Antibody (TAB-274MZ)	FC	Humanized IgG
TAB-274MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P))	FC	Humanized scFv
TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab

Mouse Antibody

CAT	Product Name	Application	Type
TAB-0565CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P))	ELISA	Mouse scFv
TAB-271MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-271MZ)	FuncS	Mouse IgG2b
TAB-273MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-273MZ)	FC, FuncS, IHC, IP, Inhib	Mouse IgG
TAB-275MZ	Mouse Anti-EGFR Recombinant Antibody (TAB-275MZ)	Cyt, FuncS, Inhib	Mouse IgG
TAB-276MZ	Anti-Human EGFR Recombinant Antibody (LA1)	Cyt, FuncS

Fc Glycosylation

CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

High-mannose Glycoform

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

Chicken IgY Antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody
BRD-0668MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

MHC Tetramer for Cancer

CAT	Product Name	Application	Type
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM
MHC-YF409	A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer	FCM

Neutralizing Antibody

CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J)	IF, FC, WB, IP, Neut	Rabbit IgG

Rabbit Monoclonal Antibody

CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
MOR-4520	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS)	IF, ICC, FC	Rabbit IgG
MOR-4570	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS)	ELISA	Rabbit IgG
MOR-4571	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG

Fab Fragment Antibody

CAT	Product Name	Application	Type
HPAB-0010-YC-F(E)	Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment	ELISA, FC	Mouse Fab
HPAB-0011-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment	FC	Human Fab
HPAB-0012-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment	FC	Human Fab
HPAB-0013-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment	FC	Human Fab
HPAB-0014-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment	FC	Human Fab

scFv Fragment Antibody

CAT	Product Name	Application	Type
FAMAB-0094-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone F4); scFv Fragment	ELISA, WB, FC	Human scFv
HPAB-0137-YJ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0137-YJ-S(P))	ELISA, FC, Inhib, FuncS	Human scFv
HPAB-S0023-YC-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone 14E1); scFv Fragment	IB, FuncS	Mouse scFv
HPAB-2059-FY-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment	FC, ELISA	Mouse scFv
HPAB-2060-FY-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment	FC, ELISA	Mouse scFv

ADCC Enhanced Antibody

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

Customer Reviews and Q&As

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

3D IHC3D Immunohistochemistry

ActivActivation

AgonistAgonist

ApopApoptosis

BABioassay

BIBioimaging

BlockBlocking

Cell ScreeningCell Screening

SeparationCell Separation

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CostimCostimulation