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    Mouse Anti-NHP EGFR Recombinant Antibody (clone 225) (CAT#: VS-1024-XY177)

    This monoclonal antibody of mouse origin is meticulously designed to target EGFR, exhibiting strong reactivity with human and non-human primate samples. Derived from purified EGFR from A431 cells, the immunogen ensures exceptional specificity. Ideal for use in immunofluorescence (IF) and immunoprecipitation (IP) applications, this antibody offers a powerful tool for studying EGFR expression and function, providing consistent performance across various research contexts requiring high precision and sensitivity.

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    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
    Normal Tissue
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Bowman's capsule
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Collecting ducts
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Distal tubules
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Proximal tubules (cell body)
    Staining:Medium
    Intensity: Strong
    Quantity: <25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
    Figure 7 Placenta
    Figure 7 Placenta
    (Cytotrophoblasts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Decidual cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Hofbauer cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - cell body
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:High
    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Non-germinal center cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

    Specifications

    • Immunogen
    • Purified EGFR from A431 cells
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • EGFR
    • Species Reactivity
    • Human, Non-human primate
    • Clone
    • 225
    • Applications
    • IF, IP

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4, with 0.2% BSA
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • IF: 5 µg/mL, IP: 2 µg/mL

    Target

    • Alternative Names
    • ERBB; ERRP; HER1; mENA; ERBB1; NNCIS; PIG61; NISBD2

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "EGFR"

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    CAT Product Name Application Type
    TAB-750 Anti-EGFR/HER1 Recombinant Antibody (TAB-750) Neut, ELISA, IF, IP, FuncS, FC, WB IgG1 - kappa
    TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
    TAB-0225CL Human Anti-EGFR Recombinant Antibody (TAB-0225CL) Block, Inhib, FuncS, Apop, In vivo Chimeric (Mouse/Human) IgG1
    PABX-051-F (E) Recombinant Mouse Anti-EGFR Antibody Fab Fragment (Matuzumab) WB, Neut, FuncS Fab
    TAB-464CQ Anti-Human EGFR Recombinant Antibody (TAB-464CQ) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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