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  • Mouse Anti-EGFR Recombinant Antibody (clone 42C11)

Mouse Anti-EGFR Recombinant Antibody (clone 42C11) (CAT#: MOB-1078z)

This product is a mouse antibody that recognizes human EGFR. It can be used in the following applications: WB, ELISA, FCM, IF, IHC, FuncS.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Formats:
  • Isotype Switching:
  • Fc Engineering:
  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Specifications

  • Immunogen
  • Human Epidermal Growth Factor Receptor
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • 42C11
  • Applications
  • WB, ELISA, FC, IF, IHC, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Functional Study.

Target

  • Alternative Names
  • EGFR; Erb-B2 Receptor Tyrosine Kinase 1; Epidermal Growth Factor Receptor; MENA; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Receptor Tyrosine-Protein Kinase ErbB-1; ERBB; ERBB1; PIG61; HER1; Erythrob

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT Product Name Application Type
TAB-750 Anti-EGFR/HER1 Recombinant Antibody (TAB-750) Neut, ELISA, IF, IP, FuncS, FC, WB IgG1 - kappa
TAB-003 Anti-Human EGFR Recombinant Antibody (Cetuximab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
TAB-H49 Anti-Human EGFR Recombinant Antibody (Modotuximab) FuncS, IF, Neut, ELISA, FC, IP, IHC IgG1 - kappa
PABX-051 Recombinant Human Anti-EGFR Antibody (Matuzumab) WB, Neut, FuncS, FC, ELISA, IF, Inhib IgG
TAB-464CQ Anti-Human EGFR Recombinant Antibody (TAB-464CQ) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

Customer Reviews and Q&As

Customer Review Q&As

Submit a review or a question


Entire Processes
The comprehensive instructions given made using the antibody simple. The testing was successful, and the suggestions were quite beneficial.

Good Signal to Noise Ratio
This antibody provides an excellent signal-to-noise ratio. It ensures that even low-abundance proteins can be detected without background noise.

Fast Shipping
The antibody was delivered efficiently and punctually. The device functioned as planned, allowing me to start using it for study right away.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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