Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) (CAT#: MOR-4520)
Recombinant Rabbit anti-EGFR monoclonal antibody for IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Host: Rabbit
Isotype: Rabbit IgG
Applications: IF, ICC, FC
Specific Inquiry
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
Specifications
- Immunogen
- Peptide corresponding to Human EGFR (aa 1193-1201)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- TH28DS
- Applications
- IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.2
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry (2-4 µg per 10^6 cells), Immunocytochemistry (2 µg/ml), Immunofluorescence (2 µg/ml).
Target
- Alternative Names
- Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EGFR"
Single-domain Antibody
| CAT | Product Name | Application | Type |
| NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) | WB, ELISA | Llama VHH |
| PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) | FuncS, ELISA, IF | Llama VHH |
| PNBL-018 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) | FuncS, SPR | Llama VHH |
| TAB-015MZ-VHH | Anti-Human EGFR Recombinant Antibody (PMP7A5) | sELISA | Single domain antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
| TAB-H35 | Anti-Human EGFR Recombinant Antibody (Futuximab) | IF, WB, Inhib | IgG1 - kappa |
| TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
| TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
| TAB-308MZ | Human Anti-EGFR Recombinant Antibody (TAB-308MZ) | FuncS | Chimeric antibody (mouse/human) |
| TAB-308MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) | ELISA, FC | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
| TAB-228CL | Anti-Human EGFR Recombinant Antibody (ABT-806) | WB, IHC | Antibody |
| TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
| TAB-297MZ | Anti-Human EGFR Recombinant Antibody (H225) | ELISA, WB | Humanized antibody |
| TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
| TAB-274MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) | FC | Humanized Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
| MOB-0242MC | Rabbit Anti-Human EGFR (phospho Y1092) Antibody | IHC, WB | |
| MOB-0243MC | Rabbit Anti-Human EGFR (phospho Y1068) Antibody | IHC, WB | |
| PABL-080 | Human Anti-EGFR Recombinant Antibody (PABL-080) | ELISA, WB, FuncS | Human IgG |
| PSBW-088 | Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment | ELISA, WB | Mouse scFv |
| TAB-477CQ | Human Anti-EGFR Recombinant Antibody (clone Laprituximab) | ELISA, IHC, FC, IP, IF, WB | Chimeric (mouse/human) IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| PFBZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528); Fab Fragment | FC | Mouse Fab |
| PFBW-039 | Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)); Fab Fragment | FuncS | Chimeric (mouse/human) Fab |
| PFBC-040 | Human Anti-EGFR Recombinant Antibody (clone MR1); Fab Fragment | Block | Human Fab |
| PFBL-459 | Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment | FC | Human Fab |
| PFBL-460 | Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment | ELISA, WB, FuncS | Humanized Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
| TAB-271MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-271MZ-F(E)) | FuncS | Mouse Fab |
| TAB-310MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-310MZ-F(E)) | ELISA | Mouse Fab |
| TAB-311MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-311MZ-F(E)) | ELISA | Mouse Fab |
| TAB-312MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-312MZ-F(E)) | ELISA | Mouse Fab |
| TAB-316MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (07D06) | WB, MTT assay |
Human Antibody
| CAT | Product Name | Application | Type |
| TAB-313MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11) | ELISA, FC | Human antibody |
| TAB-314MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-26-11) | ELISA, FC | Human antibody |
| TAB-315MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (528) | WB, FC | Human antibody |
| PABX-052-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) | WB, ELISA, FuncS | scFv |
| PABX-053-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) | WB, ELISA, FuncS | scFv |
Fc Glycosylation
| CAT | Product Name | Application | Type |
| Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
| Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
| Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
| BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
| BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
| MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
| MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
| MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
| NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
| MOR-4571 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0033-FY | Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) | ICC, IHC, WB | Rabbit IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
| AFC-TAB-710 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-753 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-H35 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Futuximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-H49 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-020 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-0120-WJ-S(P) | Human Anti-EGFR Recombinant Antibody (clone BA03); scFv Fragment | ELISA, WB | Humanized scFv |
| HPAB-0679-WJ-S(P) | Human Anti-EGFR Recombinant Antibody (clone huEGFR-7R); scFv Fragment | WB | Humanized scFv |
| HPAB-1158WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-S0022-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment | ELISA, Inhib, FuncS | Human scFv |
| HPAB-1530WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) | ELISA, WB | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot
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