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Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) (CAT#: MOR-4571)

Recombinant Rabbit anti-EGFR monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.


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Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • TH83DS
  • Applications
  • WB, IF, ICC, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Purified with Absolute Mag™ Protein A Agarose
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.09% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Flow Cytometry (1:100-1:500), Immunocytochemistry (1:100-1:1000), Immunofluorescence (1:100-1:1000) and Western Blot (1:500-1:5000).

Target

  • Alternative Names
  • Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EGFR"

Chimeric Antibody

CAT Product Name Application Type
TAB-750 Anti-EGFR/HER1 Recombinant Antibody (Futuximab/Zatuximab) Neut, ELISA, IF, IP, FuncS, FC, WB IgG1 - kappa
TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
TAB-H49 Anti-Human EGFR Recombinant Antibody (Modotuximab) FuncS, IF, Neut, ELISA, FC, IP, IHC IgG1 - kappa
TAB-0225CL-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) Block, Inhib, FuncS, Apop, In vivo Human scFv
TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
HPAB-AP881-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) ELISA, FC, IP, FuncS Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-165 Anti-Human EGFR Recombinant Antibody (Matuzumab) Neut, ELISA, IF, IP, FuncS, FC, ICC IgG1
TAB-710 Anti-EGFR Recombinant Antibody (Nimotuzumab) ELISA, IP, FC, FuncS, Neut, IF, IHC IgG1 - kappa
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-300MZ Human Anti-EGFR Recombinant Antibody (TAB-300MZ) ELISA Humanized IgG
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv

Recombinant Antibody

CAT Product Name Application Type
MOB-0242MC Rabbit Anti-Human EGFR (phospho Y1092) Antibody IHC, WB
MOB-0243MC Rabbit Anti-Human EGFR (phospho Y1068) Antibody IHC, WB
PABZ-039 Mouse Anti-EGFR Recombinant Antibody (clone mAb528) FC Mouse IgG
PABW-039 Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)) FuncS Chimeric (mouse/human) IgG
PABC-040 Human Anti-EGFR Recombinant Antibody (clone MR1) Block Human IgG

Fab Fragment Antibody

CAT Product Name Application Type
PSBW-171 Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (PSBW-171) WB Mouse scFv
HPAB-0010-YC-F(E) Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment ELISA, FC Mouse Fab
HPAB-0011-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment FC Human Fab
HPAB-0012-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment FC Human Fab
FAMAB-0202-CN-F(E) Human Anti-EGFR Recombinant Antibody (clone E10); Fab Fragment ELISA, WB Human Fab

Human Antibody

CAT Product Name Application Type
TAB-270MZ Human Anti-EGFR Recombinant Antibody (TAB-270MZ) ELISA Human antibody
TAB-278MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-278MZ-F(E)) ELISA, WB Human Fab
PABX-052-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) WB, ELISA, FuncS scFv
PABX-053-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) WB, ELISA, FuncS scFv
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Mouse Antibody

CAT Product Name Application Type
TAB-310MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-310MZ-S(P)) ELISA Mouse scFv
TAB-273MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-273MZ-F(E)) FC Mouse Fab
TAB-275MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-275MZ-F(E)) FC Mouse Fab
TAB-276MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (LA1) MTT assay
TAB-277MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (LA22) MTT assay

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4675 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-2059-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment FC, ELISA Mouse scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv
HPAB-1530WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) ELISA, WB Mouse scFv
HPAB-1531WJ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone hu340) ELISA, WB Human scFv
HPAB-1563WJ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone chMint5) ELISA, WB Human scFv

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