Anti-EGFR Recombinant Antibody (Nimotuzumab) (CAT#: TAB-710)
Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. Nimotuzumab is a humanized monoclonal antibody that as of 2014 had orphan status in the US and EU for glioma, and marketing approval in India, China, and other countries for squamous cell carcinomas of the head and neck.
Specific Inquiry
Figure 1 EGFR signal transduction and effects of EGFR inhibition on downstream targets.
Scheme of EGFR signal transduction. Nimotuzumab and PD153035 are inhibitors of EGFR: Activation of EGFR results in activation of Akt signaling which relieves a TSC1/TSC2 as well as PRAS40 (via phosphorylation of Thr246)-mediated inhibition of mTORC1. RPS6 phosphorylation at Ser235/236 and Ser 240/244 is regulated by mTORC1.
Ronellenfitsch, M. W., Zeiner, P. S., Mittelbronn, M., Urban, H., Pietsch, T., Reuter, D., ... & Harter, P. N. (2018). Akt and mTORC1 signaling as predictive biomarkers for the EGFR antibody nimotuzumab in glioblastoma. Acta neuropathologica communications, 6(1), 81.
Figure 2 EGFR signal transduction and effects of EGFR inhibition on downstream targets.
LNT-229 cells were incubated in serum-free DMEM for 90 min with vehicle (DMSO control), PD153035 (dissolved in DMSO), control solution for nimotuzumab (placebo solution of the trial) or 1 ?M nimotuzumab as indicated. Cellular lysates were analyzed by immunoblot with antibodies as indicated.
Ronellenfitsch, M. W., Zeiner, P. S., Mittelbronn, M., Urban, H., Pietsch, T., Reuter, D., ... & Harter, P. N. (2018). Akt and mTORC1 signaling as predictive biomarkers for the EGFR antibody nimotuzumab in glioblastoma. Acta neuropathologica communications, 6(1), 81.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Species Reactivity
- Human
- Applications
- ELISA, IP, FC, FuncS, Neut, IF, IHC
- Trade name
- TheraCIM
- CAS
- 780758-10-3
- Generic Name
- nimotuzumab
- Biological Half-Life
- 62–304 hours
- ATC Code
- L01XC
- MW
- 147,659.45 g/mol
- Related Disease
- Glioma cancers
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Applications
- Application Notes
- The EGFR antibody has been reported in applications of Activ, WB.
Target
- Alternative Names
- nimotuzumab;TheraCIM;780758-10-3;hR3;h-R3;TheraCIM;BIOMAb EGFR;TheraCIM;Theraloc;CIMAher;EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic le
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Please refer to Nimotuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Nimotuzumab.
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Nimotuzumab"
Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC EnhancedThis product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. Nimotuzumab is a humanized monoclonal antibody that as of 2014 had orphan status in the US and EU for glioma, and marketing approval in India, China, and other countries for squamous cell carcinomas of the head and neck.
See other products for "EGFR"
Select a product category from the dropdown menu below to view related products.
Please select product type
Chimeric Antibody
Humanized Antibody
Recombinant Antibody
scFv Fragment Antibody
Single-domain Antibody
Human Antibody
Mouse Antibody
Fc Glycosylation
High-mannose Glycoform
Deglycosylated Antibody (Non-glycosylated IgGs)
Chicken IgY Antibody
MHC Tetramer for Cancer
Neutralizing Antibody
Rabbit Monoclonal Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H35 | Anti-Human EGFR Recombinant Antibody (Futuximab) | IF, WB, Inhib | IgG1 - kappa |
| TAB-0225CL | Human Anti-EGFR Recombinant Antibody (TAB-0225CL) | Block, Inhib, FuncS, Apop, In vivo | Chimeric (Mouse/Human) IgG1 |
| TAB-308MZ | Human Anti-EGFR Recombinant Antibody (TAB-308MZ) | FuncS | Chimeric antibody (mouse/human) |
| TAB-272MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E)) | FuncS | Chimeric (mouse/human) Fab |
| TAB-302MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
Customer Reviews and Q&As
Customer Review
Q&As
-
Reliable and Sturdy
The antibody has a long shelf life and is stable for months. The lot I've been using for various experiments is still operating just as it did when it was initially used. -
Easy to Use
This antibody comes with a detailed how-to instruction and is easy to use. Every sample I tested had assays run on it, and the outcomes are repeatable and highly consistent. -
Affordable and Useful
This antibody is really inexpensive when you take into account its high quality and performance. It exceeded all of my expectations in a variety of research applications. Very strongly suggested!
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, Inhib, ICC, WB
-1-1.png)
Application: ELISA, IHC, FC, WB, IF, IA
Application: ELISA, FC, IF, WB
Application: ELISA
Application: Neut, IHC, Activ, FuncS, IF, ELISA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
