Anti-EGFR Recombinant Antibody (Nimotuzumab) (CAT#: TAB-710)

Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. Nimotuzumab is a humanized monoclonal antibody that as of 2014 had orphan status in the US and EU for glioma, and marketing approval in India, China, and other countries for squamous cell carcinomas of the head and neck.

Host: Human

Isotype: IgG1 - kappa

Applications: ELISA, IP, FC, FuncS, Neut, IF, IHC


Specific Inquiry
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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
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  • COA

Figure 1 EGFR signal transduction and effects of EGFR inhibition on downstream targets.

Scheme of EGFR signal transduction. Nimotuzumab and PD153035 are inhibitors of EGFR: Activation of EGFR results in activation of Akt signaling which relieves a TSC1/TSC2 as well as PRAS40 (via phosphorylation of Thr246)-mediated inhibition of mTORC1. RPS6 phosphorylation at Ser235/236 and Ser 240/244 is regulated by mTORC1.

Ronellenfitsch, M. W., Zeiner, P. S., Mittelbronn, M., Urban, H., Pietsch, T., Reuter, D., ... & Harter, P. N. (2018). Akt and mTORC1 signaling as predictive biomarkers for the EGFR antibody nimotuzumab in glioblastoma. Acta neuropathologica communications, 6(1), 81.

Figure 2 EGFR signal transduction and effects of EGFR inhibition on downstream targets.

LNT-229 cells were incubated in serum-free DMEM for 90 min with vehicle (DMSO control), PD153035 (dissolved in DMSO), control solution for nimotuzumab (placebo solution of the trial) or 1 ?M nimotuzumab as indicated. Cellular lysates were analyzed by immunoblot with antibodies as indicated.

Ronellenfitsch, M. W., Zeiner, P. S., Mittelbronn, M., Urban, H., Pietsch, T., Reuter, D., ... & Harter, P. N. (2018). Akt and mTORC1 signaling as predictive biomarkers for the EGFR antibody nimotuzumab in glioblastoma. Acta neuropathologica communications, 6(1), 81.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IP, FC, FuncS, Neut, IF, IHC
  • Trade name
  • TheraCIM
  • CAS
  • 780758-10-3
  • Generic Name
  • nimotuzumab
  • Biological Half-Life
  • 62–304 hours
  • ATC Code
  • L01XC
  • MW
  • 147,659.45 g/mol
  • Related Disease
  • Glioma cancers

Product Property

  • Purity
  • >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store at -20°C. Avoid multiple freeze/thaw cycles.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of Activ, WB.

Target

  • Alternative Names
  • nimotuzumab;TheraCIM;780758-10-3;hR3;h-R3;TheraCIM;BIOMAb EGFR;TheraCIM;Theraloc;CIMAher;EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic le

Related Resources

  • Biosimilar Overview
  • Related Diseases
  • Related Signaling Pathways
Please refer to Nimotuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Nimotuzumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Nimotuzumab"

Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. Nimotuzumab is a humanized monoclonal antibody that as of 2014 had orphan status in the US and EU for glioma, and marketing approval in India, China, and other countries for squamous cell carcinomas of the head and neck.

See other products for "EGFR"

Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
HPAB-AP881-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) ELISA, FC, IP, FuncS Llama VHH

Human Antibody

CAT Product Name Application Type
TAB-040 Anti-Human EGFR Recombinant Antibody (Zalutumumab) ELISA, FC, IP, FuncS, IF, Neut, ICC IgG1 - kappa
TAB-313MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11) ELISA, FC Human antibody
TAB-314MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-26-11) ELISA, FC Human antibody
TAB-315MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (528) WB, FC Human antibody
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Recombinant Antibody

CAT Product Name Application Type
MOB-0242MC Rabbit Anti-Human EGFR (phospho Y1092) Antibody IHC, WB
MOB-0243MC Rabbit Anti-Human EGFR (phospho Y1068) Antibody IHC, WB
PSBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment ELISA, WB Mouse scFv
HPAB-0044-FY Human Anti-EGFR Recombinant Antibody (clone TGM10-1) FC Humanized IgG
HPAB-0089-FY Human Anti-EGFR Recombinant Antibody (clone MIL27-3) FC Human IgG

Fab Fragment Antibody

CAT Product Name Application Type
PFBZ-039 Mouse Anti-EGFR Recombinant Antibody (clone mAb528); Fab Fragment FC Mouse Fab
PFBW-039 Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)); Fab Fragment FuncS Chimeric (mouse/human) Fab
PFBC-040 Human Anti-EGFR Recombinant Antibody (clone MR1); Fab Fragment Block Human Fab
PFBL-459 Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment FC Human Fab
PFBL-460 Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment ELISA, WB, FuncS Humanized Fab

Chimeric Antibody

CAT Product Name Application Type
TAB-0225CL Human Anti-EGFR Recombinant Antibody (TAB-0225CL) Block, Inhib, FuncS, Apop, In vivo Chimeric (Mouse/Human) IgG1
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
TAB-302MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) ELISA Chimeric (mouse/human) Fab

Humanized Antibody

CAT Product Name Application Type
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-297MZ Anti-Human EGFR Recombinant Antibody (H225) ELISA, WB Humanized antibody
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab
TAB-297MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (H225) ELISA, WB Humanized antibody
TAB-298MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (Hu225) ELISA, WB Humanized antibody

Mouse Antibody

CAT Product Name Application Type
TAB-281MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-281MZ-S(P)) WB, IF, IHC Mouse scFv
TAB-285MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-285MZ-S(P)) FC Mouse scFv
TAB-286MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-286MZ-S(P)) FC Mouse scFv
TAB-301MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-301MZ-S(P)) ELISA Mouse scFv
TAB-311MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-311MZ-F(E)) ELISA Mouse Fab

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4675 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) WB, IF, ICC, FC Rabbit IgG
MOR-4676 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0202-CN-S(P) Human Anti-EGFR Recombinant Antibody (clone E10); scFv Fragment ELISA, WB Human scFv
HPAB-S0022-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment ELISA, Inhib, FuncS Human scFv
HPAB-2059-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment FC, ELISA Mouse scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv
HPAB-2098-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment ICC, Inhib Mouse scFv

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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