Anti-Human EGFR Recombinant Antibody (Matuzumab) (CAT#: TAB-165)

Recombinant monoclonal antibody to EGFR. Matuzumab (formerly EMD 72000) is a humanized monoclonal antibody for the treatment of cancer. It binds to the epidermal growth factor receptor (EGFR) with high affinity. The mouse monoclonal antibody (mAb425).

Isotype: IgG1

Applications: Neut, ELISA, IF, IP, FuncS, FC, ICC


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Figure 1 Characterization of the EGFR-Binding and Ligand Competition Properties of Matuzumab.

(A) Surface Plasmon Resonance (SPR) analysis of the binding of sEGFR and sEGFRd3 to immobilized Fab72000 (the antigen-binding domain of matuzumab). A series of samples of sEGFR or sEGFRd3, at the indicated concentrations, was passed over a biosensor surface to which Fab72000 had been amine coupled. Data points show the equilibrium SPR response value for a representative set of samples of sEGFR (black squares) and of sEGFRd3 (open triangles), expressed as a percentage of the maximal SPR-binding response. The curves represent the fit of these data to a simple one-site Langmuir binding equation. KD values, based on at least three independent binding experiments, are 113 ± 25 nM for sEGFR and 43 ± 13 nM for sEGFRd3.
(B) Competition of EGF (green diamonds), matuzumab (red triangles), or cetuximab (black triangles) for the binding of 125I-labeled EGF to A431 cells. Cells were incubated with media containing 3 nM 125I-labeled EGF plus the indicated concentration of cold matuzumab, cetuximab, or EGF for 6 hr at 4°C. Following washing to remove unbound material, cells were lysed and liquid scintillation counting was used to determine the amount of bound 125I-labeled EGF. The counts per minute (CPM) for each sample are shown, expressed as a percentage of the CPM value obtained for no added competitor. Error bars indicate the standard deviation on three independent experiments. The line indicates the fit to a sigmoidal dose-response model. IC50 values from this analysis are 2.0 nM for matuzumab and cetuximab and 7.3 nM for EGF.

Schmiedel, J., Blaukat, A., Li, S., Knöchel, T., & Ferguson, K. M. (2008). Matuzumab binding to EGFR prevents the conformational rearrangement required for dimerization. Cancer cell, 13(4), 365-373.

Figure 2 Implications for the Mechanism of Inhibition of EGFR by Matuzumab.

(A) Cartoon of the extended sEGFR with Fab72000, in surface representation, docked onto its domain III epitope. The orientation of the receptor is the same as for the right-hand protomer in the sEGFR dimer shown in Figure 1 (with domains colored as for the left-hand protomer; EGF is omitted for clarity). The Fab72000 is colored as in Figure 3. The N-terminal region of domain I clashes with the VL domain (indicated with an arrow). Additional clashes occur along the C-terminal half of domain II (see [B]). The C-terminal loop on domain II (D278, H280) that makes critical contacts across the dimer interface is marked with an asterisk.
(B) In this view, an approximate 50° rotation about the vertical axis relative to (A), domain II is shown in sphere representation in dark green. A cartoon of domain II of the other molecule in the dimer is shown (light green) for reference. Domain I has been omitted for clarity. The VL domain of the Fab clashes with domain II in the critical C-terminal region that forms the binding pocket for the dimerization arm and makes important contacts with domain III (from N274 and E293 in domain II, colored orange). These interactions are known to be crucial for stabilizing the dimerization competent conformation of domain II. The Fab72000 epitope loop on domain III is colored in red.

Schmiedel, J., Blaukat, A., Li, S., Knöchel, T., & Ferguson, K. M. (2008). Matuzumab binding to EGFR prevents the conformational rearrangement required for dimerization. Cancer cell, 13(4), 365-373.

Figure 3 FACS analysis of REAL-Select enrichment of matuzumab-displaying cells.

Functionalized yeast cells displaying (A) matuzumab or (B) trastuzumab were mixed 1∶1,000,000 (C) and labeled using EGFR-PE and goat-anti-Fc F(ab′)2 AlexaFluor647. (C,D) FACS analysis of the cell population of sorting round 1 (C) and 4 (D) of FACS enrichment of matuzumab displaying cells.

Rhiel, L., Krah, S., Günther, R., Becker, S., Kolmar, H., & Hock, B. (2014). REAL-Select: full-length antibody display and library screening by surface capture on yeast cells. PloS one, 9(12), e114887.

Figure 4 Binding of mAbs cetuximab and matuzumab to peptide arrays.

Left: Triplicate samples of EGFR-expressing Renca-lacZ/EGFR renal carcinoma cells were treated with scFv(225)-ETA antibody-Pseudomonas exotoxin A fusion protein after preincubation of antibody-toxin with a 100-fold molar excess of synthetic peptides VLPKTLCGGGS (KTL motif) or ACKYPLGHQCGGGS (YPLG motif) as described in Supplementary Methods. Control cells were treated without antibody-toxin, with toxin without competitor (PBS) or with the irrelevant peptide SFLQDIQEV (control) as competitor. Right: Specificity was confirmed in similar experiments with ErbB2-specific scFv(FRP5)-ETA antibody-toxin and ErbB2-expressing Renca-lacZ/ErbB2 cells. The relative number of viable cells was determined after 48 h in MTT cell viability assays. Representative data from one of two independent experiments are shown. Statistically significant differences are indicated. ***P <0.001; NS, P>0.05. Data are represented as mean±s.d. Binding of mAbs cetuximab and matuzumab to synthetic peptides VLPKTLCGGGS (left) and ACKYPLGHQCGGGS (right) was measured by ELISA by adding serial dilutions of cetuximab, matuzumab or anti-ErbB2 mAb trastuzumab as control to triplicate samples of immobilized peptides.

Hartmann, C., Müller, N., Blaukat, A., Koch, J., Benhar, I., & Wels, W. S. (2010). Peptide mimotopes recognized by antibodies cetuximab and matuzumab induce a functionally equivalent anti-EGFR immune response. Oncogene, 29(32), 4517.

Figure 5 Anti-peptide antibodies bind to the surface of EGFR-expressing cells.

Control cells were incubated with matuzumab, pre-immune rabbit serum or anti-peptide antibodies pretreated with a 40-fold molar excess of specific peptide as indicated. Bound antibodies were detected with FITC-conjugated anti-rabbit IgG (anti-peptide antibodies) or anti-human IgG (matuzumab). Cell bodies were visualized by staining with an excess of propidium iodide (PI). For colocalization studies, Renca-lacZ/EGFR cells were simultaneously incubated with affinity-purified rabbit anti-KTL or anti-YPLG peptide antibodies (the latter generated by immunization with peptide ACKYPLGHQCGGGS) or pre-immune rabbit serum as control, and murine anti-EGFR mAb R1.

Hartmann, C., Müller, N., Blaukat, A., Koch, J., Benhar, I., & Wels, W. S. (2010). Peptide mimotopes recognized by antibodies cetuximab and matuzumab induce a functionally equivalent anti-EGFR immune response. Oncogene, 29(32), 4517.

Figure 6 Anti-peptide antibodies bind to the surface of EGFR-expressing cells.

Triplicate samples of A431 and MDA-MB468 cells were incubated with 50 μg/ml of anti-KTL or anti-YPLG peptide antibodies, or matuzumab, trastuzumab or pre-immune serum as indicated. The relative number of viable cells in comparison to PBS-treated controls was determined after 70 h in MTT cell viability assays. Representative data from one of two independent experiments are shown. Statistically significant differences between PBS-treated group and groups treated with antibodies are indicated. ***P<0.001; **P<0.01; NS, P>0.05. Data are represented as mean±s.d.

Hartmann, C., Müller, N., Blaukat, A., Koch, J., Benhar, I., & Wels, W. S. (2010). Peptide mimotopes recognized by antibodies cetuximab and matuzumab induce a functionally equivalent anti-EGFR immune response. Oncogene, 29(32), 4517.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Derivation
  • Humanized
  • Type
  • IgG1
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • Neut, ELISA, IF, IP, FuncS, FC, ICC
  • CAS
  • 339186-68-4
  • Generic Name
  • Matuzumab
  • Related Disease
  • Gastric cancers

Product Property

  • Purity
  • Purity >95% by SDS-PAGE.
  • Storage
  • Store at -20°C. Avoid multiple freeze/thaw cycles.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of SPR, Inhib, FC, ELISA, IF, MTT.
    ELISA: Human A431 squamous cell and MDA-MB468 breast carcinoma cells were seeded in 96-well plates at a density of 2 × 104 cells per well in the presence of 50 μg/ml of anti-peptide antibodies, or matuzumab, trastuzumab or pre-immune serum as controls.

Target

  • Alternative Names
  • Matuzumab;339186-68-4;EMD72000;MAb 425;EMD 72000;EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic leukemia viral (v erb b) oncogene homolog

Related Resources

  • Biosimilar Overview
  • Related Diseases
  • Related Signaling Pathways
Please click here for more details about the introduction to mechanism of action, clinical projects and approved drugs of Matuzumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Matuzumab"

Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to EGFR. Matuzumab (formerly EMD 72000) is a humanized monoclonal antibody for the treatment of cancer. It binds to the epidermal growth factor receptor (EGFR) with high affinity. The mouse monoclonal antibody (mAb425).

See other products for "EGFR"

Humanized Antibody

CAT Product Name Application Type
TAB-228CL Anti-Human EGFR Recombinant Antibody (ABT-806) WB, IHC Antibody
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-297MZ Anti-Human EGFR Recombinant Antibody (H225) ELISA, WB Humanized antibody
TAB-298MZ Anti-Human EGFR Recombinant Antibody (Hu225) ELISA, WB Humanized antibody
TAB-300MZ Human Anti-EGFR Recombinant Antibody (TAB-300MZ) ELISA Humanized IgG

Single-domain Antibody

CAT Product Name Application Type
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
HPAB-AP881-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) ELISA, FC, IP, FuncS Llama VHH
HPAB-AP882-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205) ELISA Llama VHH

Recombinant Antibody

CAT Product Name Application Type
PABW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7) ELISA, WB Mouse IgG
PABL-462 Human Anti-EGFR Recombinant Antibody (clone C225) FC Human IgG
PABL-463 Human Anti-EGFR Recombinant Antibody (clone h-R3) ELISA, WB, FuncS Humanized IgG
PFBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); Fab Fragment ELISA, WB Mouse Fab
PSBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment ELISA, WB Mouse scFv

Chimeric Antibody

CAT Product Name Application Type
TAB-0225CL-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) Block, Inhib, FuncS, Apop, In vivo Human scFv
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

Mouse Antibody

CAT Product Name Application Type
TAB-0564CL-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0564CL-F(E)) ELISA Mouse Fab
TAB-0565CL-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0565CL-F(E)) ELISA Mouse Fab
TAB-306MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-306MZ-F(E)) ELISA Mouse Fab
TAB-307MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-307MZ-F(E)) ELISA, FC Mouse Fab
TAB-326MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-326MZ-F(E)) FC Mouse Fab

Human Antibody

CAT Product Name Application Type
TAB-321MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-4) SPR Human antibody
TAB-322MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-6) SPR Human antibody
TAB-323MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-10) SPR Human antibody
PABX-053 Recombinant Human Anti-EGFR Antibody (DL11) WB, ELISA, FuncS IgG
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-4675 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) WB, IF, ICC, FC Rabbit IgG
MOR-4676 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) WB, IF, ICC, FC Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0060-WJ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0060-WJ-F(E)) ELISA, RIA, FC Human Fab
HPAB-2194LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E)) ELISA, WB Mouse Fab
HPAB-2195LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E)) ELISA Mouse Fab
HPAB-2196LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E)) ELISA Mouse Fab
HPAB-2197LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2197LY-F(E)) ELISA Mouse Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1519-FY-F(E) Human Anti-EGFR Recombinant Antibody (clone 15H8); scFv Fragment ELISA Human scFv
HPAB-1158WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) ELISA Mouse scFv
HPAB-S0022-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment ELISA, Inhib, FuncS Human scFv
HPAB-2059-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment FC, ELISA Mouse scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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