Mouse Anti-EGFR Recombinant Antibody (clone mAb528) (CAT#: PABZ-039)

Recombinant Mouse Antibody (mAb528) is capable of binding to EGFR, expressed in Chinese Hamster Ovary cells (CHO). The parental murine antibody, 528 IgG, inhibited the binding of m528Fv and h528Fv to EGFR expressed on cancer cells in a flow cytometric assay.

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Figure 1 Cell-surface EGFRs were measured by FACS with flow cytometry.

The cells were collected for FACS analysis of the distribution of FITC-positive cells. The data are plotted for cell numbers versus the intensity of fluorescence. Total numbers of cells counted in each cell line (stained respectively with a control IgG and the anti-EGFR mAb 528) are shown at the top.

Lu, Y., Li, X., Liang, K., Luwor, R., Siddik, Z. H., Mills, G. B., ... & Fan, Z. (2007). Epidermal growth factor receptor (EGFR) ubiquitination as a mechanism of acquired resistance escaping treatment by the anti-EGFR monoclonal antibody cetuximab. Cancer research, 67(17), 8240-8247.

Figure 2 Inhibition of EGF receptor (EGFR) activation by the ipsilateral immunoblocking with monoclonal antibody MAb528.

Monolayers were treated apically (A) and basolaterally (B) with either vehicle, 1 nM EGF alone, and 50 nM EGF alone, or with 20 nM (1:25 dilution) MAb528 (added 30 min before EGF). TER was monitored with an EVOM at indicated times after EGF treatment. Data are means ± SE of 3 replicate wells and are representative of experiments from 4 separate cell preparations.

Chen, M. C., Goliger, J., Bunnett, N., & Soll, A. H. (2001). Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability. American Journal of Physiology-Gastrointestinal and Liver Physiology, 280(2), G264-G272.


Specifications

  • Immunogen
  • Human epidermal growth factor receptor
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • mAb528
  • Applications
  • FC

Applications

  • Application Notes
  • The antibody mAb528 has been reported in applications of Flow Cytometry, Immunoprecipitation, Blocking. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
    IP: The reported concentration of mAb 528 for immunoprecipitation was 1 μg/ml.

Target

  • Alternative Names
  • EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Resources

  • Related Diseases
  • Related Signaling Pathways

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Recombinant Antibody

CAT Product Name Application Type
MOB-1078z Mouse Anti-EGFR Recombinant Antibody (clone 42C11) WB, ELISA, FC, IF, IHC, FuncS Mouse IgG1, κ
PFBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); Fab Fragment ELISA, WB Mouse Fab
HPAB-0010-YC Mouse Anti-EGFR Recombinant Antibody (clone L211C) ELISA, FC Mouse IgG
HPAB-0011-YC Human Anti-EGFR Recombinant Antibody (clone Pep 1) FC Human IgG
HPAB-0012-YC Human Anti-EGFR Recombinant Antibody (clone Pep 2) FC Human IgG

Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
TAB-015MZ-VHH Anti-Human EGFR Recombinant Antibody (PMP7A5) sELISA Single domain antibody

Humanized Antibody

CAT Product Name Application Type
TAB-228CL Anti-Human EGFR Recombinant Antibody (ABT-806) WB, IHC Antibody
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-297MZ Anti-Human EGFR Recombinant Antibody (H225) ELISA, WB Humanized antibody
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab

Chimeric Antibody

CAT Product Name Application Type
TAB-0225CL-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0225CL-F(E)) Block, Inhib, FuncS, Apop, In vivo Chimeric (Mouse/Human) Fab
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-308MZ Human Anti-EGFR Recombinant Antibody (TAB-308MZ) FuncS Chimeric antibody (mouse/human)
TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

Mouse Antibody

CAT Product Name Application Type
TAB-276MZ-S(P) Anti-Human EGFR Recombinant Antibody scFv Fragment (LA1) MTT assay
TAB-277MZ-S(P) Anti-Human EGFR Recombinant Antibody scFv Fragment (LA22) MTT assay
TAB-281MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-281MZ-S(P)) WB, IF, IHC Mouse scFv
TAB-285MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-285MZ-S(P)) FC Mouse scFv
TAB-286MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-286MZ-S(P)) FC Mouse scFv

Human Antibody

CAT Product Name Application Type
TAB-315MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (528) WB, FC Human antibody
TAB-321MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-4) SPR Human antibody
TAB-322MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-6) SPR Human antibody
PABX-053 Recombinant Human Anti-EGFR Antibody (DL11) WB, ELISA, FuncS IgG
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0727-CN-F(E) Mouse Anti-Egfr Recombinant Antibody (clone EGFR); Fab Fragment ELISA, FC, IHC, Neut Mouse Fab
HPAB-0661LY-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0661LY-F(E)) ELISA Human Fab
HPAB-0136-YJ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0136-YJ-F(E)) ELISA, FC, Inhib, FuncS Human Fab
HPAB-0137-YJ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0137-YJ-F(E)) ELISA, FC, Inhib, FuncS Human Fab
HPAB-2208LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2208LY-F(E)) ELISA, FC Mouse Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-710 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-753 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-H35 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Futuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-H49 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-020 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-AP640-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone EGL5); scFv Fragment ELISA, FC, IF, Inhib Human scFv
HPAB-S0021-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E6.3); scFv Fragment ELISA, Inhib Human scFv
HPAB-1530WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) ELISA, WB Mouse scFv
HPAB-1531WJ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone hu340) ELISA, WB Human scFv
HPAB-1563WJ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone chMint5) ELISA, WB Human scFv

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For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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