Mouse Anti-EGFR Recombinant Antibody (clone mAb528) (CAT#: PABZ-039)
Recombinant Mouse Antibody (mAb528) is capable of binding to EGFR, expressed in Chinese Hamster Ovary cells (CHO). The parental murine antibody, 528 IgG, inhibited the binding of m528Fv and h528Fv to EGFR expressed on cancer cells in a flow cytometric assay.
Specific Inquiry
Figure 1 Cell-surface EGFRs were measured by FACS with flow cytometry.
The cells were collected for FACS analysis of the distribution of FITC-positive cells. The data are plotted for cell numbers versus the intensity of fluorescence. Total numbers of cells counted in each cell line (stained respectively with a control IgG and the anti-EGFR mAb 528) are shown at the top.
Lu, Y., Li, X., Liang, K., Luwor, R., Siddik, Z. H., Mills, G. B., ... & Fan, Z. (2007). Epidermal growth factor receptor (EGFR) ubiquitination as a mechanism of acquired resistance escaping treatment by the anti-EGFR monoclonal antibody cetuximab. Cancer research, 67(17), 8240-8247.
Figure 2 Inhibition of EGF receptor (EGFR) activation by the ipsilateral immunoblocking with monoclonal antibody MAb528.
Monolayers were treated apically (A) and basolaterally (B) with either vehicle, 1 nM EGF alone, and 50 nM EGF alone, or with 20 nM (1:25 dilution) MAb528 (added 30 min before EGF). TER was monitored with an EVOM at indicated times after EGF treatment. Data are means ± SE of 3 replicate wells and are representative of experiments from 4 separate cell preparations.
Chen, M. C., Goliger, J., Bunnett, N., & Soll, A. H. (2001). Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability. American Journal of Physiology-Gastrointestinal and Liver Physiology, 280(2), G264-G272.
Specifications
- Immunogen
- Human epidermal growth factor receptor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- mAb528
- Applications
- FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody mAb528 has been reported in applications of Flow Cytometry, Immunoprecipitation, Blocking. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
IP: The reported concentration of mAb 528 for immunoprecipitation was 1 μg/ml.
Target
- Alternative Names
- EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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