Anti-Human EGFR Recombinant Antibody (Panitumumab) (CAT#: TAB-020)

Recombinant monoclonal antibody to Human EGFR. Panitumumab (INN), formerly ABX-EGF, is a fully human monoclonal antibody specific to the epidermal growth factor receptor (also known as EGF receptor, EGFR, ErbB-1 and HER1 in humans).

Host: Human

Isotype: IgG2 - kappa

Applications: ELISA, IP, FC, FuncS, Neut, IF, ICC


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 EGFR G465R mutant reveals almost complete abrogation of panitumumab and cetuximab binding.

EGFR mutation G465R abrogates binding of panitumumab and cetuximab at the protein level. Wile-type and mutant EGFR-Fc proteins were expressed and the binding of therapeutic antibodies to immobilized proteins was assessed by ELISA. Data are means from 3 experiments +/- SEM. * p<0.05, ** p<0.01, n.s. = not significant (student's T-test comparing binding of respective antibody to mutant versus wt EGFR-Fc).

Braig, F., März, M., Schieferdecker, A., Schulte, A., Voigt, M., Stein, A., ... & Engel, E. (2015). Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer. Oncotarget, 6(14), 12035.

Figure 2 EGFR G465R mutant reveals almost complete abrogation of panitumumab and cetuximab binding.

Panitumumab and cetuximab binding is abrogated in CHO cells transfected with EGFR G465R . EGFR negative CHO cells were transfected with wild type EGFR or mutants thereof. Binding of panitumumab, cetuximab or a control polyclonal EGFR antibody was assessed by FACS analysis 48h after transfection. FSC = forward scatter.

Braig, F., März, M., Schieferdecker, A., Schulte, A., Voigt, M., Stein, A., ... & Engel, E. (2015). Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer. Oncotarget, 6(14), 12035.

Figure 3 EGFR G465R mutation induces cross-resistance to panitumumab and cetuximab in an EGF-dependent Ba/ F3 cellular model.

A: EGFR signaling in EGF-dependent Ba/F3 model. Wt and S492R or G465R mutant EGFR-expressing Ba/F3 cells were cultured in the presence or absence of EGF and with addition of cetuximab, panitumumab, rituximab or erlotinib. After 2 hours, cells were harvested and EGFR/pEGFR expression analyzed by western blot analysis. B: Sensitivity of EGFR wt or EGFR G465R mutant- transfected Ba/F3 cells to treatment with EGFR-targeted antibodies. Ba/F3 cells were transformed to IL-3 independence with EGFR wt or mutant constructs and subsequently cultured in the presence or absence of EGF or with EGF in combination with panitumumab, cetuximab or control antibody rituximab. The number of viable cells was determined by trypan blue exclusion every 12 hours beginning 24 hours after seeding and plotted. Data are means from triplicate experiments +/-SEM.

Braig, F., März, M., Schieferdecker, A., Schulte, A., Voigt, M., Stein, A., ... & Engel, E. (2015). Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer. Oncotarget, 6(14), 12035.

Figure 4 In vitro EGFR targeting of panitumumab.

Immunofluorescence staining of UM-SCC-22B tumor cells and tissues for EGFR using panitumumab as the primary antibody.

Liu, Z., Liu, Y., Jia, B., Zhao, H., Jin, X., Li, F., ... & Wang, F. (2010). Epidermal growth factor receptor–targeted radioimmunotherapy of human head and neck cancer xenografts using 90Y-labeled fully human antibody panitumumab. Molecular cancer therapeutics, 1535-7163.

Figure 5 In vitro EGFR targeting of panitumumab.

In vitro inhibition of 125I-panitumumab binding to EGFR on UM-SCC-22B human head and neck cancer cells by DOTA-panitumumab and panitumumab. Points, mean (n = 3); bars, SD.

Liu, Z., Liu, Y., Jia, B., Zhao, H., Jin, X., Li, F., ... & Wang, F. (2010). Epidermal growth factor receptor–targeted radioimmunotherapy of human head and neck cancer xenografts using 90Y-labeled fully human antibody panitumumab. Molecular cancer therapeutics, 1535-7163.


Specifications

  • Immunogen
  • EGFR expressed on the surface of cells (A431 cells)
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG2 - kappa
  • Specificity
  • Tested positive against native human antigen
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IP, FC, FuncS, Neut, IF, ICC
  • Trade name
  • vectibix
  • CAS
  • 339177-26-3
  • Generic Name
  • Panitumumab
  • Biological Half-Life
  • 9.4 days (range: 4-11 days)
  • ATC Code
  • L01XC08
  • DrugBank
  • DB01269
  • UNII
  • 6A901E312A
  • ChEMBL
  • CHEMBL1201827
  • MW
  • 144,324.12 g/mol
  • Related Disease
  • Colorectal cancers

Product Property

  • Purity
  • >95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of ELISA, FC, WB, IF, Inhib.
    IF: UM-SCC-22B cell staining study was done as previously described with some modifications (17). Briefly, 70% to 85% confluent tumor cells grown in 35-mm MatTek glass-bottomed culture dishes were fixed using 4% paraformaldehyde for 10 minutes. After blocking with 10% FBS in PBS for 30 minutes, the cells were incubated with panitumumab (5 μg/mL) for 1 hour. The cells were then washed with PBS and stained with FITC-coupled mouse anti–human IgG (hIgG) for 30 minutes. After the final wash with PBS, the cells were visualized using a Leica TCS-NT confocal microscope (Wetzler).

Target

  • Alternative Names
  • Panitumumab;vectibix;339177-26-3;ABX-EGF;ABX-EGF;Vectibix;DB01269EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic leukemia viral (v erb b) o

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Panitumumab"

Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human EGFR. Panitumumab (INN), formerly ABX-EGF, is a fully human monoclonal antibody specific to the epidermal growth factor receptor (also known as EGF receptor, EGFR, ErbB-1 and HER1 in humans).

See other products for "EGFR"

Human Antibody

CAT Product Name Application Type
TAB-040 Anti-Human EGFR Recombinant Antibody (Zalutumumab) ELISA, FC, IP, FuncS, IF, Neut, ICC IgG1 - kappa
TAB-270MZ Human Anti-EGFR Recombinant Antibody (TAB-270MZ) ELISA Human antibody
TAB-299MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-299MZ-F(E)) Internalization Assay Human Fab
PABX-052-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) WB, ELISA, FuncS scFv
PABX-053-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) WB, ELISA, FuncS scFv

Recombinant Antibody

CAT Product Name Application Type
MOB-0242MC Rabbit Anti-Human EGFR (phospho Y1092) Antibody IHC, WB
MOB-0243MC Rabbit Anti-Human EGFR (phospho Y1068) Antibody IHC, WB
PABZ-039 Mouse Anti-EGFR Recombinant Antibody (clone mAb528) FC Mouse IgG
PABW-039 Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)) FuncS Chimeric (mouse/human) IgG
PABC-040 Human Anti-EGFR Recombinant Antibody (clone MR1) Block Human IgG

Single-domain Antibody

CAT Product Name Application Type
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
TAB-015MZ-VHH Anti-Human EGFR Recombinant Antibody (PMP7A5) sELISA Single domain antibody
TAB-016MZ-VHH Anti-Human EGFR Recombinant Antibody (PMP7D12) sELISA Single domain antibody

Fab Fragment Antibody

CAT Product Name Application Type
PSBW-171 Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (PSBW-171) WB Mouse scFv
HPAB-0010-YC-F(E) Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment ELISA, FC Mouse Fab
HPAB-0011-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment FC Human Fab
HPAB-0012-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment FC Human Fab
HPAB-0013-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment FC Human Fab

Mouse Antibody

CAT Product Name Application Type
TAB-0565CL-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P)) ELISA Mouse scFv
TAB-307MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-307MZ-S(P)) ELISA, FC Mouse scFv
TAB-310MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-310MZ-S(P)) ELISA Mouse scFv
TAB-311MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-311MZ-S(P)) ELISA Mouse scFv
TAB-312MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-312MZ-S(P)) ELISA Mouse scFv

Chimeric Antibody

CAT Product Name Application Type
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-308MZ Human Anti-EGFR Recombinant Antibody (TAB-308MZ) FuncS Chimeric antibody (mouse/human)
TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
TAB-272MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E)) FuncS Chimeric (mouse/human) Fab

Humanized Antibody

CAT Product Name Application Type
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-297MZ Anti-Human EGFR Recombinant Antibody (H225) ELISA, WB Humanized antibody
TAB-298MZ Anti-Human EGFR Recombinant Antibody (Hu225) ELISA, WB Humanized antibody
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-4675 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) WB, IF, ICC, FC Rabbit IgG
MOR-4676 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) WB, IF, ICC, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-S0021-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E6.3); scFv Fragment ELISA, Inhib Human scFv
HPAB-S0022-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment ELISA, Inhib, FuncS Human scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv
HPAB-2098-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment ICC, Inhib Mouse scFv
HPAB-1530WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) ELISA, WB Mouse scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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