What is the nature of this EGFR antibody?

This is a Recombinant Chimeric (human/mouse) Antibody known as Futuximab. It specifically binds to Human EGFR (HER1) and acts as an immunomodulator. It is an IgG1, kappa antibody produced in CHO cells.

Is this antibody suitable for inhibition studies?

Yes, this antibody has been reported for use in Inhibition (Inhib) assays, with a reported concentration of 50 mg/kg for certain applications. It is also validated for Immunofluorescence (IF) and Western Blot (WB).

Anti-Human EGFR Recombinant Antibody (Futuximab)

CAT#: TAB-H35

Recombinant Chimeric (human/mouse) antibody to Human EGFR. Futuximab (INN) is a chimeric monoclonal antibody designed for the treatment of cancer. It acts as an immunomodulator and also binds to HER1.

Published Data Gene Expression

Figure 1 Antibodies Internalizes and Degrades EGFRvIII.

Quantification of EGFRvIII detected in spots after antibody treatment for the indicated periods.

Keir, S. T., Chandramohan, V., Hemphill, C. D., Grandal, M. M., Melander, M. C., Pedersen, M. W., ... & Bigner, D. D. (2018). Sym004-induced EGFR elimination is associated with profound anti-tumor activity in EGFRvIII patient-derived glioblastoma models. Journal of neuro-oncology, 138(3), 489-498.

Figure 2 Antibodies Internalizes and Degrades EGFRvIII.

The cell line NR6M was treated with negative control mAb (Neg Ctrl mAb), futuximab, modotuximab, or Sym004 for 24 or 48 h, followed by harvest of cell lysates and detection of EGFRvIII by Western blot.

Inhib

Figure 3 Effect of Sym004, and Its Components Futuximab and Modotuximab, in Subcutaneous Glioblastoma Xenograft Tumor Models.

EGFRwt (D08-0308MG) patient-derived GBM xenografts passaged in athymic mice were injected s.c. in the right flank of Balb/c nu/nu mice. Sym004, futuximab, and modotuximab were administered at a dose of 50 mg/kg i.p. twice weekly for 5 weeks (10 treatments in total).

Inhib

Figure 4 Sym004 shows potent inhibition in Subcutaneous Glioblastoma Xenograft Tumor Models.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Host Species
Mouse

Derivation
Chimeric (human/mouse)

Type
IgG1 - kappa

Specificity
EGFR (epidermal growth factor receptor, receptor tyrosine-protein kinase erbB-1, ERBB1, HER1, HER-1, ERBB) [Homo sapiens]

Species Reactivity
Human

Applications
IF, WB, Inhib

CAS
1310460-85-5

Generic Name
futuximab

Related Disease
Colorectal cancers

Product Property

Purity
Purity >95% by SDS-PAGE.

Applications

Application Notes
The Human EGFR antibody has been reported in applications of Immunofluorescence, Western Blot, Inhibition.
Inhib: 50 mg/kg.

Target

Alternative Names
futuximab;1310460-85-5;992 DS;Sym004;EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic leukemia viral (v erb b) oncogene homolog (avian);prot

Gene ID
1956

UniProt ID
P00533

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Breast cancer biomarkers at key points during disease progression

Potent EGFR Inhibition

We used this Anti-Human EGFR Recombinant Antibody (Futuximab) for inhibition assays in cancer cell lines. It acted as a strong immunomodulator and effectively bound HER1. The chimeric human/mouse format was ideal for our research needs, providing reliable data on receptor blockade and downstream signaling effects.

Clear Immunofluorescence Staining

This antibody worked beautifully for Immunofluorescence (IF) staining of cell surface EGFR. The specificity to the extracellular domain was clear, with minimal background. We appreciated the high purity (>95%) of this recombinant antibody, which is essential for high-resolution imaging studies in receptor biology.

Q&As

What is the nature of this EGFR antibody?

A: This is a Recombinant Chimeric (human/mouse) Antibody known as Futuximab. It specifically binds to Human EGFR (HER1) and acts as an immunomodulator. It is an IgG1, kappa antibody produced in CHO cells.
Is this antibody suitable for inhibition studies?

A: Yes, this antibody has been reported for use in Inhibition (Inhib) assays, with a reported concentration of 50 mg/kg for certain applications. It is also validated for Immunofluorescence (IF) and Western Blot (WB).

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-H35.pdf

MSDS

MSDS - TAB-H35.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "futuximab"

Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H35)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant Chimeric (human/mouse) antibody to Human EGFR. It is a chimeric monoclonal antibody designed for the treatment of cancer. It acts as an immunomodulator and also binds to HER1.

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TAB-0564CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0564CL-F(E))	ELISA	Mouse Fab

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TAB-270MZ	Human Anti-EGFR Recombinant Antibody (TAB-270MZ)	ELISA	Human antibody

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VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

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VS-0225-XY102	CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)	FC	Mouse IgG1, kappa

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VS-0525-XY2183	Anti-Mouse EGFR Immunohistochemistry Kit	IHC

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VS-0525-YC66	Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1)	FC	Tandem scFv

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