Anti-EGFR Antibody-Cytokine Fusion Protein, IgG (Ch225)-IL2
CAT#: ACFP-SH079
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EGFR IgG to IL2. It was expressed in CHO and purified with affinity chromatography. Ch225-IL2 fusion protein bound to human EGFR with the high affinity of the parental antibody, and was as active as the equivalent amount of rhIL2. The effect of ch225-IL2 on activated T cells was most likely mediated through IL2R. This immunocytokine was designed for treating any type of solid or non-solid cancer or malignant lymphoma.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Normal Tissue
Figure 7 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
❮
❯
Specifications
- Target
- EGFR
- Molecule Class
- IgG-IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- Ch225
- Related Disease
- Any type of solid or non-solid cancer or malignant lymphoma
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
- Gene ID
- 1956
- UniProt ID
- P00533
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Related Signaling Pathways
JAK-STAT Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
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| VS-1024-XY177 | Mouse Anti-NHP EGFR Recombinant Antibody (clone 225) | IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0125-FY28 | Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera | FC, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0225-XY102 | CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY735 | Anti-EGFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY2183 | Anti-Mouse EGFR Immunohistochemistry Kit | IHC | |
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| VS-0525-YC68 | Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5) | FC | Tandem scFv |
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