Anti-Human EGFR VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Figure 1 Functional characterization of purified EgA1 ATTACK.
Biolayer interferometry (BLI)-derived sensorgrams (in black) for the interaction between immobilized EGFR-Fc and EgA1-based antibodies.
Harwood, S. L., Alvarez-Cienfuegos, A., Nuñez-Prado, N., Compte, M., Hernández-Pérez, S., Merino, N., ... & Mikkelsen, K. (2018). ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology, 7(1), e1377874.
Figure 2 Functional characterization of purified EgA1 ATTACK.
The binding to EGFR on the cell surface of HeLa cells by cetuximab- and EgA1-based antibodies at 0.1, 0.32, 1, and 10 nM, measured by FACS and normalized to the binding at 10 nM.
Harwood, S. L., Alvarez-Cienfuegos, A., Nuñez-Prado, N., Compte, M., Hernández-Pérez, S., Merino, N., ... & Mikkelsen, K. (2018). ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology, 7(1), e1377874.
Figure 3 Functional characterization of purified EgA1 ATTACK.
The binding to CD3 on the cell surface of Jurkat cells by OKT3- and EgA1-based antibodies at 0.1, 1, 3.2, and 10 nM, measured by FACS and normalized to the binding at 10 nM.
Harwood, S. L., Alvarez-Cienfuegos, A., Nuñez-Prado, N., Compte, M., Hernández-Pérez, S., Merino, N., ... & Mikkelsen, K. (2018). ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology, 7(1), e1377874.
Figure 4 EGa1-L cause a massive sequestration of cell surface EGFR.
Immediately after a 4 h incubation, cells were collected and stained for cell surface EGFR.
Oliveira, S., Schiffelers, R. M., van der Veeken, J., van der Meel, R., Vongpromek, R., en Henegouwen, P. M. V. B., ... & Roovers, R. C. (2010). Downregulation of EGFR by a novel multivalent sdAb-liposome platform. Journal of Controlled Release, 145(2), 165-175.
Figure 5 EGa1-L induce downregulation of EGFR. 14C cells were incubated for 4 h at 37 °C with liposomes containing two different ratios of sdAb to TL (0.4 and 0.8 nmol/ μmol TL) at 0.5 mM TL concentration.
Ratio's of the intensity of EGFR bands normalized to β-actin bands (non-treated set to 100%), from three independent experiments, are shown for lysates prepared after 2 days of chase following the 4 h pulse incubation.
Oliveira, S., Schiffelers, R. M., van der Veeken, J., van der Meel, R., Vongpromek, R., en Henegouwen, P. M. V. B., ... & Roovers, R. C. (2010). Downregulation of EGFR by a novel multivalent sdAb-liposome platform. Journal of Controlled Release, 145(2), 165-175.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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CAT | Product Name | Application | Type |
NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) | WB, ELISA | Llama VHH |
PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) | FuncS, ELISA, IF | Llama VHH |
HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) | ELISA, FC, IP, FuncS | Llama VHH |
HPAB-AP882-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
TAB-020 | Anti-Human EGFR Recombinant Antibody (Panitumumab) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG2 - kappa |
PABX-053 | Recombinant Human Anti-EGFR Antibody (DL11) | WB, ELISA, FuncS | IgG |
PABX-052-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) | WB, ELISA, FuncS | scFv |
PABX-053-S (P) | Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) | WB, ELISA, FuncS | scFv |
PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
TAB-165 | Anti-Human EGFR Recombinant Antibody (Matuzumab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 |
TAB-710 | Anti-EGFR Recombinant Antibody (Nimotuzumab) | ELISA, IP, FC, FuncS, Neut, IF, IHC | IgG1 - kappa |
TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
TAB-274MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) | FC | Humanized Fab |
CAT | Product Name | Application | Type |
PSBL-080 | Human Anti-EGFR Recombinant Antibody; scFv Fragment (PSBL-080) | ELISA, WB, FuncS | Human scFv |
HPAB-N0079-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone LR-DM1); scFv Fragment | IF, FuncS | Human scFv |
HPAB-N0080-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone NOV004YC); scFv Fragment | ELISA, DB, FC, Inhib | Human scFv |
HPAB-0894-WJ-S(P) | Human Anti-EGFR Recombinant Antibody (clone YZ-EGFR V1); scFv Fragment | ELISA, WB | Human scFv |
HPAB-1158WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
PABW-171 | Mouse Anti-EGFR Recombinant Antibody (PABW-171) | WB | Mouse IgG |
FAMAB-0202-CN | Human Anti-EGFR Recombinant Antibody (clone E10) | ELISA, WB | Human IgG |
HPAB-0060-WJ | Human Anti-EGFR Recombinant Antibody (HPAB-0060-WJ) | ELISA, RIA, FC | Human IgG1, κ |
HPAB-0661LY | Human Anti-EGFR Recombinant Antibody (HPAB-0661LY) | ELISA | Human IgG |
HPAB-1443LY | Human Anti-EGFR Recombinant Antibody (clone P1X) | ELISA, WB | Human IgG |
CAT | Product Name | Application | Type |
PFBL-459 | Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment | FC | Human Fab |
PFBL-460 | Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment | ELISA, WB, FuncS | Humanized Fab |
HPAB-0010-YC-F(E) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment | ELISA, FC | Mouse Fab |
HPAB-0011-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment | FC | Human Fab |
HPAB-0012-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment | FC | Human Fab |
CAT | Product Name | Application | Type |
TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
TAB-272MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) | FuncS | Mouse scFv |
TAB-302MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) | ELISA | Mouse scFv |
TAB-272MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E)) | FuncS | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
TAB-307MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-307MZ-S(P)) | ELISA, FC | Mouse scFv |
TAB-303MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-303MZ-F(E)) | ELISA | Mouse Fab |
TAB-304MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-304MZ-F(E)) | ELISA | Mouse Fab |
TAB-305MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-305MZ-F(E)) | ELISA | Mouse Fab |
TAB-306MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-306MZ-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
CAT | Product Name | Application | Type |
MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-0033-FY | Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) | ICC, IHC, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-040 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.