Human Anti-Human CD27 Recombinant Antibody (TAB-H74)
CAT#: TAB-H74
Recombinant human antibody to Human CD27. This product is designed to enhance the body's natural immune response by directly activating T cells that can specifically recognize and kill cancer cells.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Human CD27 Recombinant Antibody (TAB-H74) in SDS-PAGE
SDS-PAGE analysis of TAB-H74 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
SEC-HPLC
Figure 2 Human CD27 Recombinant Antibody (TAB-H74) in HPLC
The purity of TAB-H74 was greater than 95% as determined by SEC-HPLC.
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Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
Normal Tissue
Figure 3 High expression in lymph node.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_7_8_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139193-CD27
RNA Expression
Figure 3 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139193-CD27
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Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG1 - kappa
- Specificity
- CD27 (TNFRSF7, tumor necrosis factor receptor superfamily member 7) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- ELISA, IP, FC, FuncS, Neut, IF, ICC
- MW
- 146.0 kDa
- Related Disease
- Solid tumors
Product Property
- Purity
- >95%, by SDS-PAGE with silver staining, under reducing conditions.
Applications
- Application Notes
- The CD27 antibody has been reported in applications of ELISA, FC, S-ELISA, Stim, CFSE, Inhib, Activ.
ELISA: Purified T cells (1 × 105 T cells/well) were added to wells of a 96 well plate or 1.5 × 106 cells/well of a 24 well plate) and stimulated with Abs for different durations from 24 to 72 h at 37 °C in a humidified CO2 incubator. Cells were cultured in RPMI 1640 basal medium supplemented with 10 % heat-inactivated fetal bovine serum, L-Glutamine, and penicillin/streptomycin. Cell-free culture supernatants were obtained by centrifugation and frozen at −20 °C for cytokine and biomarker analysis.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H74, RRID: AB_3112045)
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Biosimilar Overview
Please refer to Varlilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Varlilumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-022LC | Human Anti-CD27 Recombinant Antibody (TAB-022LC) | ELISA | Humanized antibody |
| TAB-022LC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-022LC-F(E)) | ELISA | Humanized Fab |
| TAB-023LC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-023LC-F(E)) | ELISA | Humanized Fab |
| TAB-024LC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-024LC-F(E)) | ELISA | Humanized Fab |
| TAB-025LC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-025LC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-013LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-013LC-F(E)) | ELISA | Rat Fab |
| TAB-014LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-014LC-F(E)) | ELISA | Rat Fab |
| TAB-015LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-015LC-F(E)) | ELISA | Rat Fab |
| TAB-016LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-016LC-F(E)) | ELISA | Rat Fab |
| TAB-017LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-017LC-F(E)) | ELISA | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-018LC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-018LC-F(E)) | ELISA | Chimeric antibody (rat/human) |
| TAB-042LC-F(E) | Chimeric Anti-Human CD27 Recombinant Antibody Fab Fragment (5F32) | SPR, FC | Chimeric antibody (mouse/human) |
| TAB-043LC-F(E) | Chimeric Anti-Human CD27 Recombinant Antibody Fab Fragment (10F13) | SPR, FC | Chimeric antibody (mouse/human) |
| TAB-044LC-F(E) | Chimeric Anti-Human CD27 Recombinant Antibody Fab Fragment (10F31) | SPR, FC | Chimeric antibody (mouse/human) |
| TAB-045LC-F(E) | Chimeric Anti-Human CD27 Recombinant Antibody Fab Fragment (11F26) | SPR, FC | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| FN-229CQ | Hamster Anti-CD27 Recombinant Antibody (clone LG.3A10) | FC, FuncS, IHC-Fr | Armenian hamster IgG |
| HPAB-N0032-YC | Human Anti-CD27 Recombinant Antibody (HPAB-N0032-YC) | ELISA, FC | Human IgG1 |
| ZG-0801F | Mouse Anti-CD27 Recombinant Antibody (clone DEN-3) | FC | Mouse IgG1 |
| VS7-HM493 | Mouse Anti-CD27 Recombinant Antibody (clone 3B6D3) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-320CQ | Hamster Anti-CD27 Recombinant Antibody (clone LG.7F9) | FC, FuncS, Neut | Hamster IgG |
| NEUT-321CQ | Mouse Anti-CD27 Recombinant Antibody (NEUT-321CQ) | WB, Neut, FC | Mouse IgG1 |
| NEUT-322CQ | Mouse Anti-CD27 Recombinant Antibody (clone CBL803) | WB, FC, CyTOF, Neut | Mouse IgG1 |
| NEUT-323CQ | Mouse Anti-CD27 Recombinant Antibody (clone CBL185) | FC, Neut, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0547 | Hi-Affi™ Rabbit Anti-CD27 Recombinant Antibody (clone DS547AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-096LC | Human Anti-CD27 Recombinant Antibody (MOB-096LC) | ELISA, FuncS, WB | Human IgG1, κ |
| VS-0724-XY8 | Human Anti-CD27 Agonistic Antibody (VS-0724-XY8) | ELISA, Agonistic assays | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0299-CN-S(P) | Human Anti-CD27 Recombinant Antibody; scFv Fragment (HPAB-0299-CN-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-0300-CN-S(P) | Human Anti-CD27 Recombinant Antibody; scFv Fragment (HPAB-0300-CN-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-N0032-YC-S(P) | Human Anti-CD27 Recombinant Antibody; scFv Fragment (HPAB-N0032-YC-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0299-CN-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (HPAB-0299-CN-F(E)) | ELISA, FC, Block | Human Fab |
| HPAB-0300-CN-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (HPAB-0300-CN-F(E)) | ELISA, FC, Block | Human Fab |
| HPAB-N0032-YC-F(E) | Human Anti-CD27 Recombinant Antibody; Fab Fragment (HPAB-N0032-YC-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H74 | Afuco™ Anti-CD27 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H74) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY51 | AbPlus™ Anti-CD27 Magnetic Beads (2F2) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC108 | Rat Anti-CD27 Recombinant Antibody Hexamer (VS-0924-YC108), CDC Enhanced | ELISA, FC, CDC, ADCC | Antibody hexamer |
| VS-0924-YC109 | Human Anti-CD27 Recombinant Antibody Hexamer (VS-0924-YC109), CDC Enhanced | ELISA, CDC, SPR | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY30 | CytoStream™ Mouse Anti-CD27 Recombinant Antibody (VS-0225-XY30) | FC, IHC | Mouse IgG1, kappa |
| VS-0225-XY31 | CytoStream™ Mouse Anti-CD27 Recombinant Antibody (VS-0225-XY31) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY16 | Mouse Anti-CD27 scFv-Fc Chimera (VS-0425-FY16) | Inhib, ELISA, FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC688 | Recombinant Anti-CD27 Vesicular Antibody, EV Displayed (VS-0425-YC688) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1151 | Anti-Rat CD27 Immunohistochemistry Kit | IHC |
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