Human Anti-TNFSF11 Recombinant Antibody (TAB-H18) (CAT#: TAB-H18)

This product is a recombinant human antibody that recognizes TNFSF11. The antibody was purified by affinity chromatography.


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SDS-PAGE

Figure 1 Anti-Human TNFSF11 Antibody (TAB-H18) in SDS-PAGE

Figure 1 Anti-Human TNFSF11 Antibody (TAB-H18) in SDS-PAGE

SDS-PAGE analysis of TAB-H18 in non-reduced (lane 2) and reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

HPLC

Figure 2 Anti-Human TNFSF11 Antibody (TAB-H18) in HPLC

Figure 2 Anti-Human TNFSF11 Antibody (TAB-H18) in HPLC

The purity of TAB-H18 was greater than 95% as determined by SEC-HPLC.

WB

Figure 3 Anti-Human TNFSF11 Antibody (TAB-H18) in WB

Figure 3 Anti-Human TNFSF11 Antibody (TAB-H18) in WB

Western blot analysis of TAB-H18 was performed by loading Human TNFSF11 Protein (His Tag).

TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Anti-Human TNFSF11 Antibody (TAB-H18) in ELISA

Figure 4 Anti-Human TNFSF11 Antibody (TAB-H18) in ELISA

ELISA analysis of TAB-H18 was performed by coating with Human TNFSF11 Protein (His Tag). Then blocked with BSA and incubated with TAB-H18. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

DB

Figure 5 Anti-Human TNFSF11 Antibody (TAB-H18) in DB

Figure 5 Anti-Human TNFSF11 Antibody (TAB-H18) in DB

Dot Blot analysis of TAB-H18 was performed by coating with Recombinant Human TNFSF11 Protein (his Tag).

TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)


Specifications

  • Host Species
  • Human
  • Derivation
  • human
  • Type
  • Human IgG2
  • Species Reactivity
  • Human
  • Applications
  • Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, ICC and most other immunological methods.
  • MW
  • 144.7 kDa
  • Related Disease
  • Postmenopausal women osteoporosis

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TNFSF11"

Blocking Antibody

CAT Product Name Application Type
NEUT-2178CQ Rat Anti-Tnfsf11 Recombinant Antibody (clone IK22/5) Block, FC, IP, WB Rat IgG2a, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3619 Rabbit Anti-TNFSF11 Recombinant Antibody (clone DS3619AB) WB, ICC, IP Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H18 Afuco™ Anti-TNFSF11 Recombinant Antibody (AFC-TAB-H18), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC Human IgG1

Single-domain Antibody

CAT Product Name Application Type
HPAB-J0313-YC Human Anti-TNFSF11 Recombinant Single Domain Antibody (HPAB-J0313-YC) ELISA, Block Human VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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