Anti-Human VEGFA Recombinant Antibody (TAB-H73) (CAT#: TAB-H73)
Recombinant Humanized antibody to Human VEGFA
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SDS-PAGE
Figure 1 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in SDS-PAGE
SDS-PAGE analysis of TAB-H73 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 48-63 kDa and 25 kDa, respectively.
WB
Figure 2 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in WB
Western blot analysis of TAB-H73 was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H73 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
WB
Figure 3 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in WB
Western blot analysis of TAB-H73 was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H73 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
DB
Figure 4 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in DB
Dot Blot analysis of TAB-H73 was performed by coating with Human VEGF165 Protein (His Tag).
TAB-H73 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
DB
Figure 5 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in DB
Dot Blot analysis of TAB-H73 was performed by coating with Human VEGF121 Protein (His Tag).
TAB-H73 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 6 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in ELISA
ELISA analysis of TAB-H73 was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with TAB-H73. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 7 Anti-Human VEGFA Recombinant Antibody (TAB-H73) in ELISA
ELISA analysis of TAB-H73 was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with TAB-H73. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- undefined
- Derivation
- Humanized
- Type
- VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa
- Specificity
- VEGFA (vascular endothelial growth factor A, VEGF-A, VEGF) [Homo sapiens] ;
- Species Reactivity
- Human
- Applications
- WB, FC, IP, ELISA, Neut, FuncS, IF
- Related Disease
- Solid tumors
Product Property
- Purity
- >95.0% as determined by analysis by RP-HPLC.
Applications
- Application Notes
- The VEGFA antibody has been reported in applications of MRI, IHC.
MRI: Colo205 colon cancer xenografts were imaged before and 5 days after treatment with a single 10 mg kg−1 dose of either vanucizumab, bevacizumab (anti-human VEGF-A), LC06 (anti-murine/human Ang-2) or omalizumab (anti-human IgE control). Volumetric response was assessed using T2-weighted MRI, and diffusion-weighted, dynamic contrast-enhanced (DCE) and susceptibility contrast MRI used to quantify tumour water diffusivity (apparent diffusion coefficient (ADC), × 106 mm2 s−1), vascular perfusion/permeability (Ktrans, min−1) and fractional blood volume (fBV, %) respectively. Pathological correlates were sought, and preliminary gene expression profiling performed.
Target
- Alternative Names
- vanucizumab;1448221-05-3;RG7221 ;VEGFA;vascular endothelial growth factor A;vascular endothelial growth factor , VEGF;VEGF A;VPF;vascular permeability factor;VEGF;MVCD1;MGC70609;
- Gene ID
- 7422
- UniProt ID
- P15692
Related Resources
Please refer to Vanucizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Vanucizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-011 | Anti-Human VEGF Recombinant Antibody (Bevacizumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | IgG1 - kappa |
| TAB-012-F(E) | Anti-Human VEGF Recombinant Antibody Fab Fragment (TAB-012-F(E)) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Fab - G1 - kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
| TAB-009ML | Anti-Human VEGFA Recombinant Antibody scFv Fragment (TAB-009ML) | ELISA, IHC, FC, IP, IF, Inhib | scFv, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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