I am looking for an antibody against CD105. What is the host, isotype, and species reactivity of TAB-013ML?

Thank you for your question. This product, TAB-013ML, targets ENG, which is indeed also known as CD105 or Endoglin. According to our data, the Host Animal is Human, and it is a chimeric antibody. The Type is listed as IgG1, κ. The specified species reactivity and Specificity for this recombinant antibody is Human.

What applications is the Anti-Human ENG antibody (TAB-013ML) listed for, and how is it produced?

This antibody is listed for a wide variety of research applications. The data file includes ELISA, IHC, FC, IP, IF, Inhib, FuncS, WB, Block, PK, Activ, and PET. This provides broad utility for many research protocols. In terms of production, the product is a recombinant antibody and the listed expression host is CHO cells.

Anti-Human ENG Recombinant Antibody (TAB-013ML)

CAT#: TAB-013ML

Recombinant monoclonal antibody to ENG. It is a human monoclonal antibody that can be potentially used in the treatment of Hepatocellular Carcinoma, Gestational Trophoblastic Neoplasia, Choriocarcinoma, Placental Site Trophoblastic Tumor, Epithelioid Trophoblastic Tumor, Adult Solid Tumor, Advanced Soft Tissue Sarcoma, Metastatic Breast Cancer, Solid Tumors, Lung Cancer, Hepatoma, Liver Neoplasms, AdenomaLiver Cell, Carcinoma Hepatocellular, Liver Neoplasms Experimental, Adult Anaplastic Astrocytoma, Adult Anaplastic Oligodendroglioma, Adult Giant Cell Glioblastoma, Adult Gliosarcoma, Adult Mixed Glioma, Recurrent Adult Brain Neoplasm, Choriocarcinoma.

Tested Data Gene Expression

Figure 1 Anti-Human ENG Antibody (TAB-013ML) in WB

Western blot analysis of TAB-013ML was performed with Recombinant Human ENG Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-013ML and HRP mouse Anti-His tag as a secondary antibody. Chemiluminescent detection was performed.

ELISA

Figure 2 Anti-Human ENG Antibody (TAB-013ML) in ELISA

ELISA analysis of TAB-013ML was performed by coating with Recombinant Human ENG Protein. Then blocked with BSA, and incubated with Anti-Human ENG Antibody (TAB-013ML). The HRP-conjugated mouse anti-His tag as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-Human ENG Antibody (TAB-013ML) in DB

Antigen: Recombinant human ENG protein
Antibody incubation concentration: 2ng/μL.
The secondary antibody: HRP-conjugated mouse anti-His tag

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1823_F9_38_selected.jpg

Subcellular Location Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11862/158146_A_2_7_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1777_G1_13_cr59632eeaa8e82_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line SiHa

Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1759_C2_13_cr594b6f97edc10_blue_red_green.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/156475_A_9_5.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, HUVEC TERT2, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000106991-ENG

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Chimeric

Type
IgG1, κ

Specificity
Human ENG

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, Inhib, FuncS, WB, Block, PK, Activ, PET

Conjugate
Unconjugated

Related Disease
Hepatocellular Carcinoma, Gestational Trophoblastic Neoplasia, Choriocarcinoma, Placental Site Trophoblastic Tumor, Epithelioid Trophoblastic Tumor, Adult Solid Tumor, Advanced Soft Tissue Sarcoma, Metastatic Breast Cancer, Solid Tumors, Lung Cancer, Hepatoma, Liver Neoplasms, AdenomaLiver Cell, Carcinoma Hepatocellular, Liver Neoplasms Experimental, Adult Anaplastic Astrocytoma, Adult Anaplastic Oligodendroglioma, Adult Giant Cell Glioblastoma, Adult Gliosarcoma, Adult Mixed Glioma, Recurrent Adult Brain Neoplasm, Choriocarcinoma

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The ENG antibody has been reported in applications of ELISA, IHC, FC, IP, IF, Inhib, FuncS, WB, Block, PK, Activ, PET.

Target

Alternative Names
Endoglin; ORW; ORW1; Osler-Rendu-Weber syndrome 1; CD105; END; HHT1

Gene ID
2022

UniProt ID
P17813

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Breast cancer biomarkers at key points during disease progression

Excellent ENG Antibody

This is a high-quality recombinant antibody for ENG research. The product is specified as an Anti-Human ENG Recombinant Antibody and targets ENG, which is also known as CD105. We found the format, a chimeric IgG1, κ, to be suitable for our experiments. The specified purity of> 95% as determined by SDS-PAGE gave us confidence in the product's quality.

Wide Application Range

We selected this antibody for its broad utility. The product file lists an extensive range of applications, including ELISA, Flow Cytometry (FC), Immunofluorescence (IF), and Functional Studies (FuncS), which covers multiple assays in our lab. It is a chimeric antibody expressed in CHO cells and is specified to react with Human ENG. The storage instructions are clear.

Q&As

I am looking for an antibody against CD105. What is the host, isotype, and species reactivity of TAB-013ML?

A: Thank you for your question. This product, TAB-013ML, targets ENG, which is indeed also known as CD105 or Endoglin. According to our data, the Host Animal is Human, and it is a chimeric antibody. The Type is listed as IgG1, κ. The specified species reactivity and Specificity for this recombinant antibody is Human.
What applications is the Anti-Human ENG antibody (TAB-013ML) listed for, and how is it produced?

A: This antibody is listed for a wide variety of research applications. The data file includes ELISA, IHC, FC, IP, IF, Inhib, FuncS, WB, Block, PK, Activ, and PET. This provides broad utility for many research protocols. In terms of production, the product is a recombinant antibody and the listed expression host is CHO cells.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-013ML, RRID: AB_3111745)

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Biosimilar Overview

Please refer to Carotuximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Carotuximab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-013ML.pdf

MSDS

MSDS - TAB-013ML.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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