Anti-CD22 (clone RFB4)-MC-vc-PAB-MMAE ADC (CAT#: ADC-227CL)
This ADC product is composed of an anti-CD22 antibody (clone RFB4) conjugated via MC-vc-PAB linker to MMAE(anti-CD22(RFB4)-MCvcPAB-MMAE). It has demonstrated a response in treatment by a MOA (Mechanism of Action) as microtubule inhibitor.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_A_7_8.jpg
Figure 2 Spleen
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2418/6101_B_7_4.jpg
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Figure 3 RNA cell line category: Cell line enhanced (Daudi, REH, U-698, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000012124-CD22
Specifications
- Antibody Overview
- Anti-CD22 Antibody RFB4
- Clone
- RFB4
- Linker
- MC-VC-PABC (maleimidocaproyl-valine-citrulline-p-aminobenzoyloxycarbonyl)
- Linker Class/Description
- Class: Enzymatically cleavable linkers-Peptide linkers
Description: peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.
- Drug
- MMAE (Monomethyl auristatin E)
- Drug Class/Description
- Class: Auristatin
Description: Auristatins are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.
Target
- Introduction
- CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases.
CD22 is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. The presence of Ig domains makes CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
- Alternative Names
- CD22; CD22 molecule; SIGLEC2; SIGLEC-2; B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2;
- Gene ID
- 933
- UniProt ID
- P20273
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone RFB4"
See other products for "CD22"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1248z | Mouse IgG1, kappa Anti-CD22 Recombinant Antibody (MOB-1248z) | IF, FC | Mouse IgG1, κ |
| HPAB-0195-LSX | Human Anti-CD22 Recombinant Antibody (HPAB-0195-LSX) | Apop, Cyt, FC, WB, Inhib, FuncS, IF, ELISA | Human IgG |
| HPAB-0196-LSX | Mouse Anti-CD22 Recombinant Antibody (HPAB-0196-LSX) | ELISA, FC, IP, RIA | Mouse IgG |
| HPAB-0197-LSX | Human Anti-CD22 Recombinant Antibody (HPAB-0197-LSX) | ELISA, FC, FuncS | Human IgG |
| HPAB-0198-LSX | Mouse Anti-CD22 Recombinant Antibody (HPAB-0198-LSX) | ELISA, WB, IF, FC, SPR | Mouse IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1248z-S(P) | Recombinant Anti-Human CD22 Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| HPAB-0194-LSX-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0194-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0195-LSX-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0195-LSX-S(P)) | ELISA | Human scFv |
| HPAB-0370-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0370-FY-S(P)) | ELISA | Humanized scFv |
| HPAB-0371-FY-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (HPAB-0371-FY-S(P)) | Inhib | Humanized scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-183-F(E) | Anti-Human CD22 Recombinant Antibody (Moxetumomab Pasudotox) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fv - disulfide stabilized |
| TAB-1714CL | Mouse Anti-CD22 Recombinant Antibody (TAB-1714CL) | ELISA, WB | Mouse IgG |
| TAB-1728CL | Anti-Human CD22 Recombinant Antibody (RFB4) | IF, FC | |
| TAB-1714CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1714CL-S(P)) | ELISA, WB | Mouse scFv |
| TAB-1723CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1723CL-S(P)) | Inhib | Mouse scFv |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L048E | anti-CD22 immunotoxin RFB4 (scFv)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L048D | anti-CD22 immunotoxin RFB4 (scFv)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L048G | anti-CD22 immunotoxin RFB4 (scFv)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L048R | anti-CD22 immunotoxin RFB4 (scFv)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L048S | anti-CD22 immunotoxin RFB4 (scFv)-Sap | Cytotoxicity assay, Functional assay |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0196CL | Anti-Human CD22 Recombinant Antibody (hLL2) | Inhib, Cyt, FC, WB | |
| TAB-0196CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (hLL2) | ELISA, In vivo | |
| TAB-0197CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (hLL2-Cys) | ELISA, In vivo | |
| TAB-1721CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.1) | ELISA, FC, FuncS | Human antibody |
| TAB-1722CL-S(P) | Anti-Human CD22 Recombinant Antibody scFv Fragment (CD22.2) | ELISA, FC, FuncS | Human antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1724CL-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1724CL-S(P)) | Inhib | Mouse scFv |
| TAB-1724CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1724CL-F(E)) | Inhib | Chimeric (mouse/human) Fab |
| TAB-001LC | Human Anti-CD22 Recombinant Antibody (TAB-001LC) | ELISA | Chimeric antibody (mouse/human) |
| TAB-001LC-S(P) | Mouse Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-001LC-S(P)) | ELISA | Mouse scFv |
| TAB-001LC-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-001LC-F(E)) | ELISA | Chimeric antibody (mouse/human) |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1725CL-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1725CL-S(P)) | Block, Cyt, ELISA, FuncS | Humanized scFv |
| TAB-1729CL-S(P) | Human Anti-CD22 Recombinant Antibody; scFv Fragment (TAB-1729CL-S(P)) | IF, FC | Humanized scFv |
| TAB-1725CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1725CL-F(E)) | Block, Cyt, ELISA, FuncS | Humanized Fab |
| TAB-1729CL-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (TAB-1729CL-F(E)) | IF, FC | Humanized Fab |
| TAB-002LC | Anti-Human CD22 Recombinant Antibody (VM1000) | FC, ELISA, SPR, FuncS | Humanized antibody |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-001LC | Recombinant Anti-Human CD22 Antibody (Fab glycosylation) | ELISA, FC | Mouse antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-111LC | Recombinant Anti-Human CD22 Antibody (Fc glycosylation) | ELISA | Chimeric antibody (mouse/human) |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-315CQ | Rat Anti-CD22 Recombinant Antibody (clone 2D6) | Block | Rat IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0545 | Hi-Affi™ Rabbit Anti-CD22 Recombinant Antibody (clone DS545AB) | IP, WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0194-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0194-LSX-F(E)) | ELISA | Mouse Fab |
| HPAB-0195-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0195-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0196-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0196-LSX-F(E)) | ELISA | Mouse Fab |
| HPAB-0197-LSX-F(E) | Human Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0197-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0198-LSX-F(E) | Mouse Anti-CD22 Recombinant Antibody; Fab Fragment (HPAB-0198-LSX-F(E)) | ELISA, FC | Mouse Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-176 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Epratuzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-198 | Afuco™ Anti-CD22 ADCC Recombinant Antibody (Inotuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ADC-227CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
© 2023 Creative Biolabs. All Rights Reserved.
