Rabbit Anti-CD4 Recombinant Antibody (clone 2B7)

Name: Rabbit Anti-CD4 Recombinant Antibody (clone 2B7)
SKU: ZG-0589J
Rating: 5 (1 reviews)

CAT#: ZG-0589J

This product is a rabbit antibody that recognizes CD4.

Tested Data Gene Expression

Figure 1 Rabbit Anti-CD4 Antibody (ZG-0589J) in WB

Western Blot
Positive WB detected in Jurkat whole cell lysate
All lanes CD4 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa

IHC

Figure 2 Rabbit Anti-CD4 Antibody (ZG-0589J) in IHC

IHC image of ZG-0589J diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC

Figure 3 Rabbit Anti-CD4 Antibody (ZG-0589J) in IHC

IHC image of ZG-0589J diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11/if_selected.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HEL, HL-60, NB-4, THP-1, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010610-CD4

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Specifications

Immunogen
A synthesized peptide

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human CD4

Species Reactivity
Human

Clone
2B7

Applications
ELISA, WB, IHC

Conjugate
Uconjugated

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:500

Target

Alternative Names
IMD79; OKT4D; CD4mut; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3

Gene ID
920

UniProt ID
P01730

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0589J.pdf

MSDS

MSDS - ZG-0589J.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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