Mouse Anti-FN1 Recombinant Antibody (VS3-CJ306) (CAT#: VS3-CJ306)

• Immunogen
• Synthetic peptide

• Host Species
• Mouse

• Specificity
• Human FN1

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50% Glycerol, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot.

• Alternative Names
• FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF

• Gene ID
• 2335

• UniProt ID
• P02751

• Cellular Localization
• Extracellular matrix, Secreted

• Post Translation Modifications
• It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated.
  Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
  Phosphorylated by FAM20C in the extracellular medium.
  Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.
  Serotonylated on Gln residues by TGM2 in response to hypoxia.

• Protein Refseq
• NP_001293058.1; NP_001293059.1; NP_001293060.1

• Function
• Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369).
  Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369).
  Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity).
  Participates in the regulation of type I collagen deposition by osteoblasts (By similarity).
  Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
  Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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